Co-production of high-gravity bioethanol and succinic acid from potassium peroxymonosulfate and deacetylation sequentially pretreated sugarcane bagasse by simultaneous saccharification and co-fermentation

Bu, Jie; Yan, Xing; Wang, Yutao; Zhu, Siming; Zhu, Ming‐Jun

doi:10.1016/j.enconman.2019.02.038

Cited by 56 publications

(7 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, a general phenomenon was observed that a random selection of high solid loading for SSF was performed to boost ethanol concentration without statistical reasons. 6 , 15 , 22 , 23 Therefore, avoiding the selection of the solid loading for enzymatic hydrolysis and fermentation groundlessly is required.…”

Section: Introductionmentioning

confidence: 99%

“…Several strategies, including Tween 80 addition, specific bioreactor, size reduction, and fed-batch fermentation, have been investigated to block the negative interaction between lignin with cellulase and enhance enzymatic saccharification and microbial digestion at high solid loading. However, a general phenomenon was observed that a random selection of high solid loading for SSF was performed to boost ethanol concentration without statistical reasons. ,,, Therefore, avoiding the selection of the solid loading for enzymatic hydrolysis and fermentation groundlessly is required.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

High Ethanol Concentration (77 g/L) of Industrial Hemp Biomass Achieved Through Optimizing the Relationship between Ethanol Yield/Concentration and Solid Loading

Zhao

Wang

et al. 2020

ACS Omega

View full text Add to dashboard Cite

In this study, the relationships between ethanol yield/concentration and solid loading (6–21%) were investigated to enhance ethanol titer and avoid a random choice of solid loading for simultaneous saccharification and fermentation (SSF). Alkali-pretreated hemp biomass was used for SSF in four scenarios including Case I: 30 filter paper unit (FPU)-cellulase and 140 fungal xylanase unit (FXU)-hemicellulase/g-solid; Case II: 40 FPU-cellulase and 140 FXU-hemicellulase/g-solid; Case III: 30 FPU-cellulase and 140 FXU-hemicellulase/g-solid with 1% Tween80; and Case IV: 30 FPU-cellulase and 140 FXU-hemicellulase/g-solid with particle size reduction (<0.2 mm). Results showed that bioethanol yield and concentration had a negative linear ( R 2 = 0.76–0.93) and quadratic ( R 2 = 0.96–0.99) correlation with solid loading (6–21%), respectively. As compared to Case I and previous studies, an enhancement in ethanol yield and concentration through increasing cellulase dose (Case II) and adding Tween 80 (Case III) was overestimated, whereas particle size reduction (Case IV) extended the “solid effect”, evidenced by the highest ethanol concentration (77 g/L) achieved from SSF at the focus point of a quadratic model. An interpretation of the relationship between ethanol yield/concentration and solid loading not only avoids a blind selection of solid loading for SSF but also reduces extra enzymes and water consumption.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

High Ethanol Concentration (77 g/L) of Industrial Hemp Biomass Achieved Through Optimizing the Relationship between Ethanol Yield/Concentration and Solid Loading

Zhao

Wang

et al. 2020

ACS Omega

View full text Add to dashboard Cite

show abstract

“…The SHCF using Saccharomyces cerevisiae (MTCC-36) and Pichia stipitis (NCIM-3498) produced 14.25 g/L (0.1425 g/g) bioethanol at 30°C, 24 h, and pH 5.0 (under static conditions). Bu et al in a study using sugarcane bagasse achieved 0.36-g/g ethanol yield from its holocellulose [57]. The efficiency of bioethanol production process using DS as LB source was found to be 95.68% based on the TRS conversion efficiency.…”

Section: Mass/materials Balance During Bioethanol Productionmentioning

confidence: 97%

“…Sanchez et al obtained a bioethanol yield of 17.1 g/L using the SHF and 15.0 g/L using the SSF with 1 × 10 7 cell/mL of Saccharomyces cerevisiae and an enzyme loading of 25 FPU/g using pine (Pinus pseudostrobus L.) sawdust as substrate [56]. In another study, a very high-ethanol titer of 120.77 g/L and a fermentation efficiency of 79.01% were achieved at the enzyme loading of 15 FPU/g substrate using sugarcane bagasse by the SHCF [57]. Once the yeast cells have achieved sufficient biomass, their metabolic functions are diverted towards bioethanol production in the presence of fermentable sugars in the medium (29.20 g/L).…”

Section: Separate Hydrolysis and Co-fermentation For Bioethanol Productionmentioning

confidence: 97%

Response surface methodology (RSM) for optimization of thermochemical pretreatment method and enzymatic hydrolysis of deodar sawdust (DS) for bioethanol production using separate hydrolysis and co-fermentation (SHCF)

Raina

Slathia

Sharma

2020

Biomass Conv. Bioref.

View full text Add to dashboard Cite

In the present work, Cedrus deodara (deodar) sawdust abbreviated as DS was subjected to thermochemical pretreatment followed by enzymatic hydrolysis and fermentation for bioethanol production. Response surface methodology (RSM) based on central composite design (CCD) tool was employed to optimize dilute acid pretreatment method (at 121°C temperature and 1-bar pressure) and enzymatic hydrolysis processes. Factorial design of experiments used chemical concentration, incubation time, and biomass loading to optimize thermochemical pretreatment method using dilute acid concentration. Maximum total reducing sugar (TRS) concentration (13.62 g/L) was obtained using optimized conditions (1.5% HCl concentration, 10% biomass loading and 30-min incubation time). These significant studies were subjected to optimize enzymatic hydrolysis process using the CCD tool of the RSM where cellulase loading, xylanase loading, pH, and temperature were taken into consideration. TRS concentration obtained was 29.20 g/L after 48 h with enzyme loading of 9 U/g biomass, each for cellulase and xylanase enzyme at pH 5.0 and 30°C temperature. Fermentation conditions for enzymatically hydrolyzed DS revealed that at 10% (v/v) yeast inoculum concentrations (Saccharomyces cerevisiae (MTCC-36) and Pichia stipitis (NCIM-3498)) after 24-h fermentation time and pH 5.0, bioethanol concentration was 14.25 g/L with 95.68% conversion efficiency. Characterization studies for native and pretreated DS by Fourier transform infrared spectroscopy (FTIR), powder X-ray diffraction (PXRD), and scanning electron microscopy (SEM) analyses revealed delignification rate of 30.93% of DS biomass under optimized thermochemical pretreatment conditions. Statistical studies for optimization of pretreatment and enzymatic hydrolysis for bioethanol production stated DS sawdust (a furniture industry waste) as a potential substrate for biofuel production.

show abstract

“…This culturing method is indispensable when, for example, a substrate used in high concentrations has an inhibiting effect on the cultured microorganism and the productivity of the process (the ratio of the amount of product formed to the amount of substrate consumed) does not allow a desirable concentration of succinic acid to be produced from the initially added substrate [14][15][16]. A new approach involves the co-production of succinic acid and ethanol by simultaneous saccharification and co-fermentation [17]. However, in this process, alcohol accounted for the large majority of the product, 120 g/L, while the succinic acid concentration was 34.84 g/L.…”

Section: Introductionmentioning

confidence: 99%

A New Strategy for Effective Succinic Acid Production by Enterobacter sp. LU1 Using a Medium Based on Crude Glycerol and Whey Permeate

et al. 2019

View full text Add to dashboard Cite

The newly-isolated strain Enterobacter sp. LU1, which has previously been shown to be an effective producer of succinic acid on glycerol with the addition of lactose, was used for further intensive works aimed at improving the production parameters of the said process. The introduction of an initial stage of gentle culture aeration allowed almost 47 g/L of succinic acid to be obtained after 168 h of incubation, which is almost two times faster than the time previously taken to obtain this amount. Furthermore, the replacement of glycerol with crude glycerin and the replacement of lactose with whey permeate allowed the final concentration of succinic acid to be increased to 54 g/L. Considering the high content of yeast extract (YE) in the culture medium, tests were also performed with a reduced YE content via its partial substitution with urea. Although this substitution led to a deterioration of the kinetic parameters of the production process, using the fed-batch strategy, it allowed a succinic acid concentration of 69 g/L to be obtained in the culture medium, the highest concentration ever achieved using this process. Furthermore, the use of microaerophilic conditions meant that the addition of lactose to the medium was not required, with 37 g/L of succinic acid being produced on crude glycerol alone.

show abstract

Co-production of high-gravity bioethanol and succinic acid from potassium peroxymonosulfate and deacetylation sequentially pretreated sugarcane bagasse by simultaneous saccharification and co-fermentation

Cited by 56 publications

References 50 publications

High Ethanol Concentration (77 g/L) of Industrial Hemp Biomass Achieved Through Optimizing the Relationship between Ethanol Yield/Concentration and Solid Loading

High Ethanol Concentration (77 g/L) of Industrial Hemp Biomass Achieved Through Optimizing the Relationship between Ethanol Yield/Concentration and Solid Loading

Response surface methodology (RSM) for optimization of thermochemical pretreatment method and enzymatic hydrolysis of deodar sawdust (DS) for bioethanol production using separate hydrolysis and co-fermentation (SHCF)

A New Strategy for Effective Succinic Acid Production by Enterobacter sp. LU1 Using a Medium Based on Crude Glycerol and Whey Permeate

Contact Info

Product

Resources

About