Co-segregation analysis and mapping of the anthracnose Co-10 and angular leaf spot Phg-ON disease-resistance genes in the common bean cultivar Ouro Negro

Abstract

Anthracnose (ANT) and angular leaf spot (ALS) are devastating diseases of common bean (Phaseolus vulgaris L.). Ouro Negro is a highly productive common bean cultivar, which contains the Co-10 and Phg-ON genes for resistance to ANT and ALS, respectively. In this study, we performed a genetic co-segregation analysis of resistance to ANT and ALS using an F2 population from the Rudá × Ouro Negro cross and the F2:3 families from the AND 277 × Ouro Negro cross. Ouro Negro is resistant to races 7 and 73 of the ANT and race 63-39 of the ALS pathogens. Conversely, cultivars AND 277 and Rudá are susceptible to races 7 and 73 of ANT, respectively. Both cultivars are susceptible to race 63-39 of ALS. Co-segregation analysis revealed that Co-10 and Phg-ON were inherited together, conferring resistance to races 7 and 73 of ANT and race 63-39 of ALS. The Co-10 and Phg-ON genes co-segregated and were tightly linked at a distance of 0.0 cM on chromosome Pv04. The molecular marker g2303 was linked to Co-10 and Phg-ON at a distance of 0.0 cM. Because of their physical linkage in a cis configuration, the Co-10 and Phg-ON resistance alleles are inherited together and can be monitored with great efficiency using g2303. The close linkage between the Co-10 and Phg-ON genes and prior evidence are consistent with the existence of a resistance gene cluster at one end of chromosome Pv04, which also contains the Co-3 locus and ANT resistance quantitative trait loci. These results will be very useful for breeding programs aimed at developing bean cultivars with ANT and ALS resistance using marker-assisted selection.

Genetic analysis of the response to eleven Colletotrichum lindemuthianum races in a RIL population of common bean (Phaseolus vulgaris L.)

“…On this position were mapped the well-known resistance genes Co-3 and Co-9 [14]. In this LG, but in a distal position from Co-9 , was mapped Co-10 from cultivar Ouro Negro as a single gene conferring resistance against races 23, 64, 73, 81 and 89 [22,24]. To date, independence between Co-10 and resistance genes included in the Co-3 cluster has not been clearly established.…”

Common bean reaction to angular leaf spot comprises transcriptional modulation of genes in the ALS10.1 QTL

“…For instance, one of the most important sources of resistance to ALS, the AND 277 genotype, carries the Phg -1 a , Phg-2 2 , Phg-3 2 , and Phg-4 2 loci (Mahuku et al 2004; Caixeta et al, 2005; Gonçalves-Vidigal et al, 2011). Dominant monogenic inheritance for resistance to ALS has also been reported for other resistance sources (Namayanja et al, 2006; Amaro et al, 2007; Silva et al, 2008; Gonçalves-Vidigal et al, 2013). Gene characterization or functional analyses, however, have never been conducted to understand the function of the genes present in these regions.…”

“…For instance, an R gene cluster was located on chromosome Pv04 (David et al, 2009; Geffroy et al, 2009), with the discovery of surrounding genes that are differentially expressed under biotic and abiotic stresses (David et al, 2010). This bean chromosome also harbors resistance genes against several strains of Colletotrichum lindemuthianum and Pseudocercospora griseola (López et al, 2003; Oblessuc et al, 2012, 2014; Ferreira et al, 2013; Gonçalves-Vidigal et al, 2013). …”

Alternative markers linked to the Phg-2 angular leaf spot resistance locus in common bean using the Phaseolus genes marker database

“…Mesoamerican resistance locus, Phg-2, mapped qualitatively in Mexico 54 is located on chromosome 8 at a distance of 5.9 and 11.8 cM from markers SN02 and OPE04, respectively (Sartorato et al, 2000). The Mesoamerican ALS resistance locus, Phg-3, mapped in accession Ouro Negro, is linked to marker g2303 at a distance of 0 cM (Gonçalves-Vidigal et al, 2013). Two dominant, monogenic, resistance loci were also identified in G10909: Phg G10909A and Phg G10909B (Mahuku et al, 2011).…”