Co-selective Pressure of Cadmium and Doxycycline on the Antibiotic and Heavy Metal Resistance Genes in Ditch Wetlands

Yu, Meng‐Fei; Shu, Bizhi; Li, Zhixuan; Liu, Guihua; Liu, Wenzhi; Yang, Yuyi; Ma, Lin

doi:10.3389/fmicb.2022.820920

Cited by 5 publications

(4 citation statements)

References 37 publications

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“…carrying bla TEM , copA, copB, pcoD , and zntA . Experiments using microcosms of wetland sediments demonstrated through metagenomic qPCR that doxycycline (50 mg l −1 ) and cadmium (0.5–5 mg l −1 ) exposure for 3 months increased MRG and ARG relative abundance more than either agent on their own (Yu et al 2022 ). In this study, both agents were positively correlated with individual resistance genes and bacterial genera, with Acinetobacter being one notable predicted host for MRGs, ARGs, and integrons.…”

Section: Evidence For Co-resistancementioning

confidence: 99%

Unravelling the mechanisms of antibiotic and heavy metal resistance co-selection in environmental bacteria

Gillieatt,

Coleman

2024

FEMS Microbiology Reviews

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The co-selective pressure of heavy metals is a contributor to the dissemination and persistence of antibiotic resistance genes in environmental reservoirs. The overlapping range of antibiotic and metal contamination and similarities in their resistance mechanisms point to an intertwined evolutionary history. Metal resistance genes are known to be genetically linked to antibiotic resistance genes, with plasmids, transposons, and integrons involved in the assembly and horizontal transfer of the resistance elements. Models of co-selection between metals and antibiotic have been proposed, however the molecular aspects of these phenomena are in many cases not defined or quantified and the importance of specific metals, environments, bacterial taxa, mobile genetic elements, and other abiotic or biotic conditions are not clear. Co-resistance is often suggested as a dominant mechanism, but interpretations are beset with correlational bias. Proof of principle examples of cross-resistance and co-regulation have been described but more in-depth characterisations are needed, using methodologies that confirm functional expression of resistance genes and that connect genes with specific bacterial hosts. Here, we comprehensively evaluate the recent evidence for different models of co-selection from pure culture and metagenomic studies in environmental contexts and we highlight outstanding questions.

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Section: Evidence For Co-resistancementioning

confidence: 99%

Unravelling the mechanisms of antibiotic and heavy metal resistance co-selection in environmental bacteria

Gillieatt,

Coleman

2024

FEMS Microbiology Reviews

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“…Metal resistance determinants may be found on the chromosome or in clusters on bacterial plasmids. Sometimes these heavy metal resistance genes are found on the same plasmids as multiple antimicrobial resistance (AMR) determinants [102,103]. Efflux pumps work in conjunction with other resistance mechanisms such as genes coding for metal oxidases and often these genes are located on the same operon and are subject to the similar levels of regulation.…”

Section: Heavy Metal Resistancementioning

confidence: 99%

Efflux-mediated tolerance to cationic biocides, a cause for concern?

Wand¹,

Sutton²

2022

Microbiology

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With an increase in the number of isolates resistant to multiple antibiotics, infection control has become increasingly important to help combat the spread of multi-drug-resistant pathogens. An important component of this is through the use of disinfectants and antiseptics (biocides). Antibiotic resistance has been well studied in bacteria, but little is known about potential biocide resistance genes and there have been few reported outbreaks in hospitals resulting from a breakdown in biocide effectiveness. Development of increased tolerance to biocides has been thought to be more difficult due to the mode of action of biocides which affect multiple cellular targets compared with antibiotics. Very few genes which contribute towards increased biocide tolerance have been identified. However, the majority of those that have are components or regulators of different efflux pumps or genes which modulate membrane function/modification. This review will examine the role of efflux in increased tolerance towards biocides, focusing on cationic biocides and heavy metals against Gram-negative bacteria. As many efflux pumps which are upregulated by biocide presence also contribute towards an antimicrobial resistance phenotype, the role of these efflux pumps in cross-resistance to both other biocides and antibiotics will be explored.

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“…Notably, heavy metals and antibiotics can induce cross-resistance, and thus, bacteria can become resistant to both. Toxic metals are assumed to exert selective pressure on metal-resistant bacteria, and genes associated with heavy metals, such as arsenic and cadmium, are frequently found in plasmids associated with ARGs [ 12 , 13 ]. Another study found that arsenic stress increases ARG abundance and dissemination risk [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Response of Antibiotic Resistance Genes and Related Microorganisms to Arsenic during Vermicomposting of Cow Dung

Chen

Zhang

et al. 2022

IJERPH

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Antibiotic resistance pollution in livestock manure is a persistent issue that has drawn public attention. Vermicomposting is an ecofriendly biological process that can render livestock manure harmless and resourceful. However, little is known about the impact of vermicomposting on antibiotic resistance in livestock manure under stress caused by potentially toxic arsenic levels. Herein, lab-scale vermicomposting was performed to comprehensively evaluate the shift in antibiotic resistance genes (ARGs) and related microorganisms in fresh earthworm casts as well as vermicompost product health (i.e., nutrient availability and enzyme activity) when they were fed on arsenic-contaminated cow manure. The results showed that the earthworms’ interaction with cow dung led to a significant reduction in ARG concentrations, especially for tetracycline ARGs (tet-ARGs), β-lactam ARGs (bla-ARGs), and quinolone ARGs (qnr-ARGs). However, arsenic significantly enhanced ARG accumulation in earthworm casts in a dose-dependent manner. Moreover, vermicomposting increased the percentage of Bacteroidota in the converted products. Furthermore, arsenic exposure at low concentrations promoted the proliferation of Proteobacteria, whereas high concentrations had little effect on Proteobacteria. Our study provides valuable insight into the changes in the antibiotic resistome and related microorganisms during vermicomposting of arsenic-amended cow manure, and it is crucial to explain the environmental impact of earthworms and improve our understanding of the reciprocal benefits of soil invertebrates.

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Co-selective Pressure of Cadmium and Doxycycline on the Antibiotic and Heavy Metal Resistance Genes in Ditch Wetlands

Cited by 5 publications

References 37 publications

Unravelling the mechanisms of antibiotic and heavy metal resistance co-selection in environmental bacteria

Unravelling the mechanisms of antibiotic and heavy metal resistance co-selection in environmental bacteria

Efflux-mediated tolerance to cationic biocides, a cause for concern?

Response of Antibiotic Resistance Genes and Related Microorganisms to Arsenic during Vermicomposting of Cow Dung

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