Co-sequencing and novel delayed anti-correlation identify function for pancreatic enriched microRNA biomarkers in a rat model of acute pancreatic injury

Li, Zhihua; Rouse, Rodney

doi:10.1186/s12864-018-4657-2

Cited by 5 publications

(4 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results are consistent with the fact that miR-217, -216a, and -216b are generated from a common host gene designated as MIR217HG, which spans an approximately 40-kbp region in the rat, mouse, and human genomes, allowing simultaneous regulation at a transcriptional level. Furthermore, the expression of MIR217HG-derived miRNAs is simultaneously dysregulated in different tissues under pathological conditions, such as in acute pancreatic injury [38][39][40][41][42][43], pancreatic cancer [44,45], and gastric cancers [46]; this may also be the case with podocyte injury.…”

Section: Discussionmentioning

confidence: 99%

The Role of miR-217-5p in the Puromycin Aminonucleoside-Induced Morphological Change of Podocytes

Ishibashi¹,

Hayashi²,

Horikawa³

et al. 2022

ncRNA

View full text Add to dashboard Cite

Podocytes, alternatively called glomerular epithelial cells, are terminally differentiated cells that wrap around glomerular capillaries and function as a part of the glomerular filtration barrier in the kidney. Therefore, podocyte injury with morphological alteration and detachment from glomerular capillaries leads to severe proteinuria and subsequent renal failure through glomerulosclerosis. Previous RNA sequencing analysis of primary rat podocytes exposed to puromycin aminonucleoside (PAN), a well-known experimental model of injured podocytes, identified several transcripts as being aberrantly expressed. However, how the expression of these transcripts is regulated remains unclear. MicroRNAs (miRNAs) are small noncoding RNAs that posttranscriptionally inhibit the expression of their target transcripts. In this study, using small RNA sequencing analysis, miR-217-5p was identified as the most upregulated transcript in PAN-treated rat podocytes. MiR-217-5p overexpression in E11 podocyte cells led to shrunken cells with abnormal actin cytoskeletons. Consistent with these changes in cell morphology, gene ontology (GO) enrichment analysis showed that interactive GO terms related to cell morphogenesis were enriched with the predicted targets of miR-217-5p. Of the predicted targets highly downregulated by PAN, Myosin 1d (Myo1d) is a nonmuscle myosin predicted to be involved in actin filament organization and thought to play a role in podocyte morphogenesis and injury. We demonstrated that miR-217-5p targets Myo1d by luciferase assays, qRT–PCR, and Western blotting. Furthermore, we showed that miR-217-5p was present in urine from PAN- but not saline-administrated rats. Taken together, our data suggest that miR-217-5p may serve as a therapeutic target and a biomarker for podocyte injury.

show abstract

Section: Discussionmentioning

confidence: 99%

The Role of miR-217-5p in the Puromycin Aminonucleoside-Induced Morphological Change of Podocytes

Ishibashi¹,

Hayashi²,

Horikawa³

et al. 2022

ncRNA

View full text Add to dashboard Cite

show abstract

“…Gene expression data for the high-dose rats and their controls were obtained from a separate study generated from the same experimental animals and reported by Li and Rouse. 15 Next-generation sequencing was used to determine gene expression levels in the cerulein-treated rats in comparison with control rats across a time series, and the resulting Reg3γ data was extracted to support the present study. Gene expression in log fold change above control exhibited the same trend and was significantly correlated with IHC percent positive results across all time points.…”

Section: Discussionmentioning

confidence: 99%

“…Reg3γ IHC results in rats were compared with gene expression data for Reg3γ obtained from the same pancreatic tissue and quantified through next generation sequencing as described in Li and Rouse. 15 Gene expression levels were only determined for the high-dose rats and their control groups. Sequencing of messenger RNA (mRNA) samples and initial quality control bioinformatic analysis were performed by Quintiles, Inc (Morrisville, NC).…”

Section: Next-generation Sequencing and Data Processingmentioning

confidence: 99%

Detection of Reg3γ by Immunohistochemistry in Cerulein-Induced Model of Acute Pancreatic Injury in Mice and Rats

Shea

Rouse²

2019

Pancreas

Self Cite

View full text Add to dashboard Cite

In a continuation of previous work, Reg3γ protein was further evaluated as a biomarker of pancreatic injury using immunohistochemistry in an additional species.Methods: Mice and rats were treated with intraperitoneal cerulein injections, creating acute pancreatic injury. Mice received 2, 4, or 6 doses, and rats received 1, 2, or 3 doses of cerulein creating low, medium, and high treatment groups. Control animals were dosed with phosphate-buffered saline at corresponding volumes and intervals. Groups of 6 animals were killed 1, 3, 6, 24, and 48 hours after final treatments. Reg3γ immunohistochemical staining and image analysis were performed on pancreatic tissue obtained 6, 24, or 48 hours after control or cerulein treatment. Staining was quantified using image analysis software to calculate area of positivity as a percentage of total tissue area.Results: Percent positivity of Reg3γ in both species rose by 6 hours, peaked by 24 hours across all 3 cerulein doses, and dropped significantly by 48 hours. In high-dose rats with accompanying gene expression data, Reg3γ gene expression corresponded temporally with quantitative staining data.Conclusions: Reg3γ staining quantified through image analysis showed a time-and dose-response in cerulein-treated mice and rats.

show abstract

“…Extensive studies have been conducted in the past decade to discover the involvement of miRNAs in pancreatitis. Overwhelming experimental evidence demonstrated the functional significance of miRs in AP and CP using various model systems [ 157 ].…”

Section: Biomarkers and Therapeutic Approachesmentioning

confidence: 99%

The Role of MicroRNAs in Pancreatitis Development and Progression

Patel

Almanzar

LaComb

et al. 2023

IJMS

View full text Add to dashboard Cite

Pancreatitis (acute and chronic) is an inflammatory disease associated with significant morbidity, including a high rate of hospitalization and mortality. MicroRNAs (miRs) are essential post-transcriptional modulators of gene expression. They are crucial in many diseases’ development and progression. Recent studies have demonstrated aberrant miRs expression patterns in pancreatic tissues obtained from patients experiencing acute and chronic pancreatitis compared to tissues from unaffected individuals. Increasing evidence showed that miRs regulate multiple aspects of pancreatic acinar biology, such as autophagy, mitophagy, and migration, impact local and systemic inflammation and, thus, are involved in the disease development and progression. Notably, multiple miRs act on pancreatic acinar cells and regulate the transduction of signals between pancreatic acinar cells, pancreatic stellate cells, and immune cells, and provide a complex interaction network between these cells. Importantly, recent studies from various animal models and patients’ data combined with advanced detection techniques support their importance in diagnosing and treating pancreatitis. In this review, we plan to provide an up-to-date summary of the role of miRs in the development and progression of pancreatitis.

show abstract

Co-sequencing and novel delayed anti-correlation identify function for pancreatic enriched microRNA biomarkers in a rat model of acute pancreatic injury

Cited by 5 publications

References 71 publications

The Role of miR-217-5p in the Puromycin Aminonucleoside-Induced Morphological Change of Podocytes

The Role of miR-217-5p in the Puromycin Aminonucleoside-Induced Morphological Change of Podocytes

Detection of Reg3γ by Immunohistochemistry in Cerulein-Induced Model of Acute Pancreatic Injury in Mice and Rats

The Role of MicroRNAs in Pancreatitis Development and Progression

Contact Info

Product

Resources

About