The output flow pulsation characteristics of the hydraulic pump due to the structural characteristics may cause pump source fluid pressure pulsation and even cause the equipment to vibrate, which will affect the life and working reliability of the equipment. Scholars have done a lot of theoretical and simulation analysis on the characteristics of fluid flow and pressure pulsation caused by the specific structure and structure of the plunger pump, but there are few comparisons and analyses of the simplified model of the plunger pump and the pressure pulsation characteristics with experiments. In this paper, AMESim software is utilized to establish a simplified model of one seven-plunger hydraulic pump, and simulate and analyze the pump source fluid pressure pulsation characteristics of different system load pressures at a constant speed. An experimental platform for testing pump fluid pressure pulsation was designed and built, and the actual measurement and simulation results of pump fluid pressure pulsation were compared and analyzed. The results show that the system simulation data is in good agreement with the measured data, which verifies the correctness of the simplified model of the plunger pump. At the same time, it is found that the fluid pressure pulsation of the pump source exhibits broadband and multi-harmonic characteristics. At a constant speed, as the load pressure of the hydraulic system increases, the pump source fluid pressure pulsation amplitude increases, the pressure pulsation rate decreases, and the impact on the fundamental frequency amplitude is the most significant. The research results can provide a theoretical basis for suppressing the pressure pulsation of the pump source fluid and reducing the vibration response of a hydraulic pipeline under the action of the pulsating harmonic excitation.