CO<sub>2</sub> Concentration in <i>Chlamydomonas reinhardtii</i>: Effect of the Pyrenoid Starch Sheath

Mukherjee, Ananya

doi:10.1104/pp.20.00267

Cited by 1 publication

(1 citation statement)

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“…So the material and energy exchange mechanism between thylakoid-membrane light-reaction and pyrenoid dark-reaction in C. pyrenoidosa cell may be different from that of in Chlamydomonas reinhardtii cell. The function of starch sheath is to prevent the leakage of CO 2 from the pyrenoid and to minimize the oxygen exposure in the pyrenoid matrix (45, 46). Therefore, the pyrenoid, starch sheath, and thylakoid membranes jointly consist of the Ci transport and utilization system, connecting the light-dependent and light-independent reactions.…”

Section: Discussionmentioning

confidence: 99%

In situ3D comparison ofChlorella pyrenoidosawith nuclear-irradiated mutagenic strains by using focused ion beam milling and cryo-electron tomography

Feng

Wang

et al. 2020

Preprint

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Microalgae are highly efficient photosynthetic organisms that hold enormous potential as sources of renewable energy. In particular, Chlorella pyrenoidosa displays a rapid growth rate, high tolerance to light, and high lipid content, making it especially valuable for applications such as flue gas CO2 fixation, biofuel production, and nutritional extracts. In order to unveil its full potential, it is necessary to characterize its subcellular architecture. Here, we achieved three-dimensional (3D) visualization of the architectures of C. pyrenoidosa cells, by combining focused ion beam scanning electron microscopy (FIB/SEM), cryo-FIB milling, and cryo-electron tomography (cryo-ET). These high-resolution images bring to light intricate features of intact organelles, including thylakoid membranes, pyrenoid, starch granules, mitochondria, nucleus, lipid droplets and vacuoles, as well as the fine architectures within the chloroplast, including the concave-convex pyrenoid, plastoglobules, thylakoid tips, and convergence zones. Significantly, comparative analysis of wild-type and nuclear-irradiated mutagenic strains determined that cell volume and surface area of mutant cells have increased substantially to 2.2-fold and 1.7-fold, respectively, consistent with up-regulation of the enzyme Rubisco and enhanced photosynthetic metabolic processes. Moreover, quantitative analysis established that the thylakoid membrane width in mutant cells increased to 1.3-fold, while the membrane gap decreased to 0.8-fold, possibly contributing to the higher biomass growth rate of mutant cells. Our work reveals the first 3D subcellular architectures of C. pyrenoidosa cell and provides a structural framework for unlocking the higher growth rate in microalgae relevant to a wide range of industrial applications.

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Section: Discussionmentioning

confidence: 99%

In situ3D comparison ofChlorella pyrenoidosawith nuclear-irradiated mutagenic strains by using focused ion beam milling and cryo-electron tomography

Feng

Wang

et al. 2020

Preprint

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CO₂ Concentration in Chlamydomonas reinhardtii: Effect of the Pyrenoid Starch Sheath

Cited by 1 publication

References 10 publications

In situ3D comparison ofChlorella pyrenoidosawith nuclear-irradiated mutagenic strains by using focused ion beam milling and cryo-electron tomography

In situ3D comparison ofChlorella pyrenoidosawith nuclear-irradiated mutagenic strains by using focused ion beam milling and cryo-electron tomography

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CO2 Concentration in Chlamydomonas reinhardtii: Effect of the Pyrenoid Starch Sheath

Cited by 1 publication

References 10 publications

In situ3D comparison ofChlorella pyrenoidosawith nuclear-irradiated mutagenic strains by using focused ion beam milling and cryo-electron tomography

In situ3D comparison ofChlorella pyrenoidosawith nuclear-irradiated mutagenic strains by using focused ion beam milling and cryo-electron tomography

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CO₂ Concentration in Chlamydomonas reinhardtii: Effect of the Pyrenoid Starch Sheath