Co-substrate facilitated charge transfer for bioelectricity evolution in a toxic blue-green alga-fed microbial fuel cell technology

Ndayisenga, Fabrice; Yu, Zhisheng; Kabera, Telesphore; Wang, Bobo; Liang, Hongxia; Phulpoto, Irfan Ali; Habiyakare, Telesphore; Kalisa, Ndayambaje Yvan; Yan, Xinyu

doi:10.1007/s10098-021-02173-1

Cited by 6 publications

(3 citation statements)

References 33 publications

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“…Although, single substrate feeding is often conventionally favored in biotechnological applications, it could lead to multiple metabolic constraints, mainly due to the fine coordination required to simultaneously balance global cell metabolism, growth and overproduction of target compounds ( Babel, 2009 ; Liu, Santala and Stephanopoulos, 2020 ; Hartline et al, 2021 ). On the other hand, several experimental efforts have already demonstrated that is possible overcome these limitations by adopting metabolite doping or co-substrate feeding, accordingly with particular metabolic engineering applications ( Papazi et al, 2012 ; Anfelt et al, 2015 ; Liu et al, 2019a ; Park et al, 2019 ; Gu et al, 2020 ; Ndayisenga et al, 2021 ), in agreement with the present work. Additionally, thanks to the very poor nutritional requirements of cyanobacteria, the BG11 growth medium is still suitable for further supplementation, in the light of large-scale application.…”

Section: Discussionsupporting

confidence: 89%

“…However, single substrate feeding is conventionally preferred but naturally elicits stoichiometric constraints on available carbon atoms, energy and redox state, leading to metabolic imbalance, when a specific route has to be exploited. Also, in cyanobacterial and microalgal cultivations, co-substrate feeding or nutrient modulation strategies have been adopted ( Papazi et al, 2012 ; Anfelt et al, 2015 ; Ndayisenga et al, 2021 ). In comparison to carbohydrate-using microorganisms, cyanobacteria may represent a more sustainable alternative because, drawing energy from photosynthesis, they avoid expensive fermentable sugars as carbon feedstock and can be exploited for CO 2 capture and conversion.…”

Section: Introductionmentioning

confidence: 99%

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Combining metabolite doping and metabolic engineering to improve 2-phenylethanol production by engineered cyanobacteria

Usai

Cordara

et al. 2022

Front. Bioeng. Biotechnol.

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2-Phenylethanol (2-PE) is a rose-scented aromatic compound, with broad application in cosmetic, pharmaceutical, food and beverage industries. Many plants naturally synthesize 2-PE via Shikimate Pathway, but its extraction is expensive and low-yielding. Consequently, most 2-PE derives from chemical synthesis, which employs petroleum as feedstock and generates unwanted by products and health issues. The need for “green” processes and the increasing public demand for natural products are pushing biotechnological production systems as promising alternatives. So far, several microorganisms have been investigated and engineered for 2-PE biosynthesis, but a few studies have focused on autotrophic microorganisms. Among them, the prokaryotic cyanobacteria can represent ideal microbial factories thanks to their ability to photosynthetically convert CO2 into valuable compounds, their minimal nutritional requirements, high photosynthetic rate and the availability of genetic and bioinformatics tools. An engineered strain of Synechococcus elongatus PCC 7942 for 2-PE production, i.e., p120, was previously published elsewhere. The strain p120 expresses four heterologous genes for the complete 2-PE synthesis pathway. Here, we developed a combined approach of metabolite doping and metabolic engineering to improve the 2-PE production kinetics of the Synechococcus elongatus PCC 7942 p120 strain. Firstly, the growth and 2-PE productivity performances of the p120 recombinant strain were analyzed to highlight potential metabolic constraints. By implementing a BG11 medium doped with L-phenylalanine, we covered the metabolic burden to which the p120 strain is strongly subjected, when the 2-PE pathway expression is induced. Additionally, we further boosted the carbon flow into the Shikimate Pathway by overexpressing the native Shikimate Kinase in the Synechococcus elongatus PCC 7942 p120 strain (i.e., 2PE_aroK). The combination of these different approaches led to a 2-PE yield of 300 mg/gDW and a maximum 2-PE titer of 285 mg/L, 2.4-fold higher than that reported in literature for the p120 recombinant strain and, to our knowledge, the highest recorded for photosynthetic microorganisms, in photoautotrophic growth condition. Finally, this work provides the basis for further optimization of the process aimed at increasing 2-PE productivity and concentration, and could offer new insights about the use of cyanobacteria as appealing microbial cell factories for the synthesis of aromatic compounds.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Combining metabolite doping and metabolic engineering to improve 2-phenylethanol production by engineered cyanobacteria

Usai

Cordara

et al. 2022

Front. Bioeng. Biotechnol.

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“…A renewable energy approach may employ proton exchange membrane fuel cells (PEMFCs) (Tongphanpharn et al 2021; Chidanand and Eswara Prasad 2021), in particular microbial fuel cells (MFCs) (Ndayisenga et al 2021). PEMFCs have an estimated market of US$2.10 billion in 2021 up to US$22.74 billion in 2028(Fortune Business Insights 2021).…”

Section: Introductionmentioning

confidence: 99%

Production of Bacterial Cellulose and its Evaluation as a Proton Exchange Membrane

Ramírez-Carmona

Gálvez-Gómez

González-Perez

et al. 2022

Preprint

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Production of bacterial cellulose and its evaluation as a proton exchange membrane (PEM) was evaluated. Initially, the bacterial cellulose (BC) was produced by fermentation in a 600 mL bioreactor with a 300 mL medium volume, 10% v/v inoculum with Komagataeibacter hansenii under static conditions, and a temperature of 30°C. The bacteria were cultivated in Hestrin-Schramm (HS) medium with pH adjustment to 6.6 with HCl and/or NaOH. Five culture media were evaluated: HS (M1), M1 + green tea extract (M3), M1 + mixture of extra thyme and green tea (M4), and M1 + glycerin (M5). The kinetics of BC production was followed by digital images. Subsequently, BC production cellulose was carried out using M5 under the same operating conditions. After 3, 5, 10 and 13 days of fermentation, the thickness of formed BC formed was measured, respectively, as 0.301 ± 0.008 cm, 0.552 ± 0.026 cm, 0.584 ± 0.03 cm and 0.591 ± 0.018 cm. Finally, BC was characterized by porosity, water absorption capacity, ion exchange capacity, mechanical strength and diffusivity. The results showed that thinner membranes favor the processes of ion exchange (0.143 H+mmol g− 1) and water absorption (93%). On the other hand, thicker membranes enhance physical parameters of transport across the membrane and its operability. Nevertheless, BC membranes can be a good alternative as PEM once they are functionalized.

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Production of Bacterial Cellulose Hydrogel and its Evaluation as a Proton Exchange Membrane

et al. 2023

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Production of bacterial cellulose hydrogel and its evaluation as a proton exchange membrane (PEM) was evaluated. Initially, the bacterial cellulose hydrogel membranes (BCH) was produced by fermentation in a 600 mL bioreactor with a 300 mL medium volume, 10% v/v inoculum with Komagataeibacter hansenii under static conditions, and a temperature of 30 °C. The bacteria were cultivated in Hestrin-Schramm (HS) medium with pH adjustment to 6.6 with HCl and/or NaOH. Five culture media were evaluated to obtain uniformity on the surface and a rapid formation of BCH membrane: HS (M1), M1 + green tea extract (M3), M1 + mixture of extra thyme and green tea (M4), and M1 + glycerin (M5). The kinetics of BCH production was followed by digital images. Subsequently, BCH production cellulose was carried out using M5 under the same operating conditions. After 3, 5, 10 and 13 days of fermentation, the thickness of BCH formed was measured, respectively, as 0.301 ± 0.008 cm, 0.552 ± 0.026 cm, 0.584 ± 0.03 cm and 0.591 ± 0.018 cm. Finally, BCH was characterized by porosity, water absorption capacity, ion exchange capacity, mechanical strength and diffusivity. The results showed that thinner membranes favor the processes of ion exchange (0.143 H+mmol g−1) and water absorption (93%). On the other hand, thicker membranes enhance physical parameters of transport across the membrane and its operability. Nevertheless, BCH membranes can be a good alternative as PEM to microbial fuel cell once they are functionalized.

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Co-substrate facilitated charge transfer for bioelectricity evolution in a toxic blue-green alga-fed microbial fuel cell technology

Cited by 6 publications

References 33 publications

Combining metabolite doping and metabolic engineering to improve 2-phenylethanol production by engineered cyanobacteria

Combining metabolite doping and metabolic engineering to improve 2-phenylethanol production by engineered cyanobacteria

Production of Bacterial Cellulose and its Evaluation as a Proton Exchange Membrane

Production of Bacterial Cellulose Hydrogel and its Evaluation as a Proton Exchange Membrane

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