CO2 concentration change in Nannochloropsis sp. culture medium

Yamasaki, Shigehisa; Hirata, Hachirô

doi:10.1016/0144-8609(94)00010-x

Cited by 16 publications

(10 citation statements)

References 5 publications

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“…There is minimal difference in cell densities following the exponential growth phase, indicating that the algal culture is either light or nutrient limited. Previous experiments have shown that Nannochloropsis can be grown effectively over a wide temperature range: between 15 and 30°C [4], [32], [33]. Our study shows that N. oculata also copes well with natural diel variations in temperature between 15 and 25°C.…”

Section: Resultssupporting

confidence: 69%

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The Effect of Diel Temperature and Light Cycles on the Growth of Nannochloropsis oculata in a Photobioreactor Matrix

et al. 2014

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A matrix of photobioreactors integrated with metabolic sensors was used to examine the combined impact of light and temperature variations on the growth and physiology of the biofuel candidate microalgal species Nannochloropsis oculata. The experiments were performed with algal cultures maintained at a constant 20°C versus a 15°C to 25°C diel temperature cycle, where light intensity also followed a diel cycle with a maximum irradiance of 1920 µmol photons m−2 s−1. No differences in algal growth (Chlorophyll a) were found between the two environmental regimes; however, the metabolic processes responded differently throughout the day to the change in environmental conditions. The variable temperature treatment resulted in greater damage to photosystem II due to the combined effect of strong light and high temperature. Cellular functions responded differently to conditions before midday as opposed to the afternoon, leading to strong hysteresis in dissolved oxygen concentration, quantum yield of photosystem II and net photosynthesis. Overnight metabolism performed differently, probably as a result of the temperature impact on respiration. Our photobioreactor matrix has produced novel insights into the physiological response of Nannochloropsis oculata to simulated environmental conditions. This information can be used to predict the effectiveness of deploying Nannochloropsis oculata in similar field conditions for commercial biofuel production.

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Section: Resultssupporting

confidence: 69%

“…The optimum temperature for Nannochloropsis sp. has been reported as 24–26°C [32], [33]. The effect of temperature variations on the growth rate and photosynthetic performance of Nannochloropsis sp.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Diel Temperature and Light Cycles on the Growth of Nannochloropsis oculata in a Photobioreactor Matrix

et al. 2014

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“…In the pre-culture, CO 2 concentration may be limited because the 2-day pre-culture reached to the cell concentration of 5 × 10 6 cells mL −1 (Yamasaki & Hirata, 1995). The dilution of pre-culture by inoculation might cause the increase of available CO 2 to the cells and lead to the EPA accumulation by the aeration of ambient air.…”

Section: Discussionmentioning

confidence: 99%

Accumulation of eicosapentaenoic acid in Nannochloropsis sp. in response to elevated CO2 concentrations

et al. 2005

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To increase eicosapentaenoic acid (EPA, 20:5, n-3) content in the marine alga Nannochloropsis sp., the effect of CO 2 concentration during cultivation has been investigated. In a batch culture under normal atmospheric conditions (0.037% CO 2 ), the EPA content per cell increased during the first 1.5 days and then decreased immediately even though the cells were in an exponential growth phase. Increasing the CO 2 concentration to 0.3% and 2% over day 1.5 retained the EPA content at the higher concentration for another 1 and 2 days, respectively, suggesting that the EPA accumulation is enhanced by elevated concentrations of CO 2 . EPA accumulation in response to elevated CO 2 concentrations was also observed during a later growth phase when CO 2 was introduced after the decrease of EPA content. The addition of CO 2 caused a slight decrease in the pH of the medium though this was not the cause of the observed EPA accumulation as addition of acidic buffer did not affect the EPA content. The maximum EPA production was obtained when 2% CO 2 was supplied 12 h prior to the end of the exponential growth. The total EPA production during 4-day cultivation was about twice that obtained with ambient air. These results suggest that the available CO 2 concentration affects the EPA content in Nannochloropsis sp.

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“…oculata fell below 1.0 mM on the third day, which was when the density of N . oculata was approximately 13 × 10 6 cells/mL, 2 a good growth of N . oculata might be obtained by supplying CO 2 gas, 1,7 sodium acetate, 8,9 sodium bicarbonate, 10 etc.…”

mentioning

confidence: 95%

“…Dissolved inorganic carbon (DIC) is an essential substance for algal growth in water 1 . As the density of Nannochloropsis oculata increases, the concentration of DIC decreases 2 . It is considered that the low DIC concentration inhibits the growth of N .…”

mentioning

confidence: 99%

Effect of dissolved inorganic carbon concentration on oxygen production of a unicellular alga Nannochloropsis oculata

Yamasaki

Yamaoka

2001

Fisheries Sci

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CO2 concentration change in Nannochloropsis sp. culture medium

Cited by 16 publications

References 5 publications

The Effect of Diel Temperature and Light Cycles on the Growth of Nannochloropsis oculata in a Photobioreactor Matrix

The Effect of Diel Temperature and Light Cycles on the Growth of Nannochloropsis oculata in a Photobioreactor Matrix

Accumulation of eicosapentaenoic acid in Nannochloropsis sp. in response to elevated CO2 concentrations

Effect of dissolved inorganic carbon concentration on oxygen production of a unicellular alga Nannochloropsis oculata

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