Coacervates of whey proteins to protect and improve the oral delivery of a bioactive molecule

Chapeau, Anne-Laure; Bertrand, Nicolas; Briard‐Bion, Valérie; Hamon, Pascaline; Poncelet, Denis; Bouhallab, Saı̈d

doi:10.1016/j.jff.2017.09.009

Cited by 22 publications

(9 citation statements)

References 51 publications

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“…Recent studies showed that complex coacervation could be used by mixing two oppositely charged proteins with an affinity for an encapsulated bioactive component [ 106 ]. Efficient encapsulation yield with a protective effect was reported for vitamin B9 encapsulated in milk-derived protein coacervates.…”

Section: Stability and Encapsulation Of Eosmentioning

confidence: 99%

Essential Oils in Livestock: From Health to Food Quality

et al. 2021

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Using plant essential oils (EOs) contributes to the growing number of natural plants’ applications in livestock. Scientific data supporting the efficacy of EOs as anti-inflammatory, antibacterial and antioxidant molecules accumulates over time; however, the cumulative evidence is not always sufficient. EOs antioxidant properties have been investigated mainly from human perspectives. Still, so far, our review is the first to combine the beneficial supporting properties of EOs in a One Health approach and as an animal product quality enhancer, opening new possibilities for their utilization in the livestock and nutrition sectors. We aim to compile the currently available data on the main anti-inflammatory effects of EOs, whether encapsulated or not, with a focus on mammary gland inflammation. We will also review the EOs’ antioxidant activities when given in the diet or as a food preservative to counteract oxidative stress. We emphasize EOs’ in vitro and in vivo ruminal microbiota and mechanisms of action to promote animal health and performance. Given the concept of DOHaD (Developmental Origin of Health and Diseases), supplementing animals with EOs in early life opens new perspectives in the nutrition sector. However, effective evaluation of the significant safety components is required before extending their use to livestock and veterinary medicine.

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Section: Stability and Encapsulation Of Eosmentioning

confidence: 99%

Essential Oils in Livestock: From Health to Food Quality

et al. 2021

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“…These types of materials have the advantage of allowing for fully-aqueous processing, and have been used broadly for protein purification, with coacervates finding particular utility in delivery. [45][46][47][48][49][50] Furthermore, the phase-separated nature of these materials allows for strong partitioning of proteins into the polymeric phase, allowing for the easy preparation formulations with a high encapsulation and/or loading of protein. [12,[51][52][53] These high levels of incorporation are mainly due to the electrostatics and entropic gains associated with phase separation, but also take advantage of differences in the overall polarity of the coacervate environment compared to pure water.…”

Section: The Challenge Of Protein Encapsulationmentioning

confidence: 99%

“…Complex coacervation, [49,51,52,56,57,[61][62][63][64][65][66][67][68][69][70][71][72][73] whose characteristics mimic the characteristics of cells, is a simple, purely aqueous strategy for encapsulating proteins. Complex coacervation is a liquid-liquid phase separation phenomenon resulting from the association of two oppositely charged macro-ions (e.g., polymer, protein, surfactant micelles).…”

Section: Complex Coacervation Phase Behavior and Biomimicrymentioning

confidence: 99%

Protein Encapsulation Using Complex Coacervates: What Nature Has to Teach Us

McTigue

Perry

2020

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Protein encapsulation is a growing area of interest, particularly in the fields of food science and medicine. The sequestration of protein cargoes has been achieved using a variety of methods, each with benefits and drawbacks. One of the most significant challenges associated with protein encapsulation has been achieving high loading while maintaining protein viability. This difficulty has been exacerbated because many encapsulant systems require the use of organic solvents. In contrast, nature has optimized strategies to compartmentalize and protect proteins inside the cell-a purely aqueous environment. Although the mechanisms whereby aspects of the cytosol is able to stabilize proteins are unknown, the crowded nature of many newly discovered, liquid phase separated 'membraneless organelles' that achieve protein compartmentalization suggests that the material environment surrounding the protein may be critical in determining stability. Here, we focus on encapsulation strategies based on liquid-liquid phase separation, and complex coacervation in particular, which has many of the key features of the cytoplasm as a material. We review the literature on protein encapsulation via coacervation and discuss the parameters relevant to creating protein-containing coacervate formulations. Additionally, we highlight potential opportunities associated with the creation of tailored materials to better facilitate protein encapsulation and stabilization.

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“…Finally, heteroprotein systems that undergo complex coacervation are also of great interest to the field [48,64,65,66]. These multi-protein coacervates are particularly relevant to the formation and biological function of membraneless organelles [67,68].…”

Section: Macrophase Separation Of Protein–polyelectrolyte Complexesmentioning

confidence: 99%

Macro- and Microphase Separated Protein-Polyelectrolyte Complexes: Design Parameters and Current Progress

2019

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Protein-containing polyelectrolyte complexes (PECs) are a diverse class of materials, composed of two or more oppositely charged polyelectrolytes that condense and phase separate near overall charge neutrality. Such phase-separation can take on a variety of morphologies from macrophase separated liquid condensates, to solid precipitates, to monodispersed spherical micelles. In this review, we present an overview of recent advances in protein-containing PECs, with an overall goal of defining relevant design parameters for macro- and microphase separated PECs. For both classes of PECs, the influence of protein characteristics, such as surface charge and patchiness, co-polyelectrolyte characteristics, such as charge density and structure, and overall solution characteristics, such as salt concentration and pH, are considered. After overall design features are established, potential applications in food processing, biosensing, drug delivery, and protein purification are discussed and recent characterization techniques for protein-containing PECs are highlighted.

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Coacervates of whey proteins to protect and improve the oral delivery of a bioactive molecule

Cited by 22 publications

References 51 publications

Essential Oils in Livestock: From Health to Food Quality

Essential Oils in Livestock: From Health to Food Quality

Protein Encapsulation Using Complex Coacervates: What Nature Has to Teach Us

Macro- and Microphase Separated Protein-Polyelectrolyte Complexes: Design Parameters and Current Progress

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