Coacervation of Polyelectrolyte-Protein Complexes

Dubin, Paul L.; Ross, T. D.; Sharma, Indu; Yegerlehner, B. E.

doi:10.1021/bk-1987-0342.ch008

Cited by 24 publications

(18 citation statements)

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“…These materials have recently been used in biomedical and pharmaceutical applications because chitosan is biocompatible, biodegradable and non-toxic. [1] Alginate is a linear co-polymer comprised of b-D mannuronic and a-L-guluronic acids linked by (1)(2)(3)(4) bonds forming homopolymeric blocks called M or G and heterpolymeric blocks called MG. Due to its gelling capacity in the presence of divalent cations, it has been used in numerous applications in the food, cosmetics and pharmaceutical industries. [2] Polyelectrolyte complexes are formed by mixing solutions of macromolecules with opposite charges.…”

Section: Introductionmentioning

confidence: 99%

“…[2] Polyelectrolyte complexes are formed by mixing solutions of macromolecules with opposite charges. They have been proposed for the design of drug-release systems, [3] protein separation, [4] anticoagulant coatings, [5] and substance-separation membranes. [6] In previous papers we have dealt with polyelectrolyte complexes (PEC) of chitosan and some anionic polysaccharides including carboxymethyl cellulose, [7,8] polygalacturonic acid and k-carrageenan.…”

Section: Introductionmentioning

confidence: 99%

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Diffusion Through Membranes of the Polyelectrolyte Complex of Chitosan and Alginate

Cárdenas

Argüelles‐Monal

Goycoolea

et al. 2003

Macromolecular Bioscience

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The swelling of membranes of the polyelectrolyte complex (PEC) between chitosan and alginate shows a similar pattern to that of other PECs. However, if the swelled membranes are dried, a second swelling process is seen which exhibits Fickian behavior. The apparent activation energy was estimated to be 32.8 kJ · mol−1. The release rate of model solutes was highly dependent on their molecular weight and the pH of the medium.Arrhenius type plot of the temperature dependence of the apparent diffusion coefficients for the membrane of the polyelectrolyte complex between chitosan and alginate in water.magnified imageArrhenius type plot of the temperature dependence of the apparent diffusion coefficients for the membrane of the polyelectrolyte complex between chitosan and alginate in water.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Diffusion Through Membranes of the Polyelectrolyte Complex of Chitosan and Alginate

Cárdenas

Argüelles‐Monal

Goycoolea

et al. 2003

Macromolecular Bioscience

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“…Thus, protein complexation with polymers in aqueous solution may be driven by hydrogen bonding, 1 hydrophobic interaction, 2 and electrostatic forces. 3 Hydrogen bonding can occur only under stringent conditions since it requires not only the presence of hydrogen-bond donors and acceptors, but also the spatial alignment of the donor-acceptor pairs. Consequently, electrostatic and hydrophobic interactions are more common factors in protein-polyelectrolyte complexation.…”

Section: Introductionmentioning

confidence: 99%

Binding of proteins to copolymers of varying hydrophobicity

Gao

Dubin

1999

Biopolymers

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“…These interactions are modulated by such variables as pH and ionic strength, and may result in soluble complexes, 1,2 complex coacervation, [3][4][5][6] or the formation of amorphous precipitates. [7][8][9] Protein-polyelectrolyte complexation can change the activity of catalytic proteins (enzymes), 10 -12 alter ligand binding to transport proteins, 1,7 and stabilize biological activity against temperature change.…”

Section: Introductionmentioning

confidence: 99%

Light scattering, CD, and ligand binding studies of ferrihemoglobin-polyelectrolyte complexes

et al. 1999

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Quasi-elastic light scattering (QELS), electrophoretic light scattering (ELS), CD spectroscopy, and azide binding titrations were used to study the complexation at pH 6.8 between ferrihemoglobin and three polyelectrolytes that varied in charge density and sign. Both QELS and ELS show that the structure of the soluble complex formed between ferrihemoglobin and poly(diallyldimethylammonium chloride) [PDADMAC] varies with protein concentration. At fixed 1.0 mg/mL polyelectrolyte concentration, protein addition increases complex size and decreases complex mobility in a tightly correlated manner. At 1.0 mg/mL or greater protein concentration, a stable complex is formed between one polyelectrolyte chain and many protein molecules (i.e., an intrapolymer complex) with apparent diameter approximately 2.5 times that of the protein-free polyelectrolyte. Under conditions of excess polyelectrolyte, each of the three ferrihemoglobin-polyelectrolyte solutions exhibits a single diffusion mode in QELS, which indicates that all protein molecules are complexed. CD spectra suggest little or no structural disruption of ferrihemoglobin upon complexation. Azide binding to the ferrihemoglobin-poly(2-acrylamide-2-methylpropanesulfonate)

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Coacervation of Polyelectrolyte-Protein Complexes

Cited by 24 publications

References 0 publications

Diffusion Through Membranes of the Polyelectrolyte Complex of Chitosan and Alginate

Diffusion Through Membranes of the Polyelectrolyte Complex of Chitosan and Alginate

Binding of proteins to copolymers of varying hydrophobicity

Light scattering, CD, and ligand binding studies of ferrihemoglobin-polyelectrolyte complexes

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