Coagulase gene polymorphisms of Staphylococcus aureus isolates from patients at Kosti Teaching Hospital, Sudan

Abstract

Background Staphylococcus aureus is a common cause of nosocomial infections. Epidemiological typing of S. aureus enables control of its spread. The objective of this study was to investigate coagulase gene polymorphisms of S. aureus isolated from patients at Kosti Hospital in Sudan. Methods In total, 110 S. aureus isolates were recovered from 110 patients who were enrolled in the study. S. aureus strains were isolated on blood agar and MacConkey agar and then identified by conventional tests. Resistance to methicillin was determined by detection of the mecA gene. Polymorphism in the coagulase gene ( coa ) was investigated using PCR followed by Alu I RFLP analysis. Results Methicillin-resistant S. aureus accounted for 62/110 (56 %) of the isolates. PCR of the coa gene showed two different amplicons, one of 680 bp detected in 83/110 (75.5 %) of the isolates and one of 790 bp detected in 27/110 (24.5 %). When digested with the Alu I enzyme, the 790 bp amplicon resulted in three restriction fragments of 500, 210 and 80 bp ( coa 1). Restriction of the 680 bp amplicon gave two patterns; the first ( coa 2) was found in 22/110 (20 %) of the isolates and showed four fragments of 210, 210, 180 and 80 bp, and the second ( coa 3) was found in 61/110 (55.5 %) and revealed three fragments of 390, 210 and 80 bp. Most of the coa 3 isolates (75.4%) were methicillin-resistant. Conclusion Three polymorphic genotypes of S. aureus were identified in patients at Kosti Hospital. The coa 3 genotype was the predominant one and was mostly detected in methicillin-resistant isolates.

Emergence of CC8/ST239- SCCmec III/t421 tigecycline resistant and CC/ST22-SCCmec IV/t790 vancomycin resistant Staphylococcus aureus strains isolated from wound: A two-year multi-center study in Tehran, Iran

“…Similarly, Afrogh et al [27] reported six different patterns of coa gene among S. aureus strains isolated from staff nose and patients in Iran. However, other similar results were achieved by Younis Omar et al [28] and Ibrahim et al [29]. In contrast, a study performed by Abdulghany et al [30] distinguished 15 different coa types among 58 MRSA isolates.…”

Emerging Challenges in Staphylococcus aureus Bloodstream Infections: Insights from Coagulase Typing, Toxin Genes, and Antibiotic Resistance Patterns

“…In an Egyptian study conducted by Abdulghani and Khairy on 58 MRSA strains recovered from clinical isolates, 15 diferent coa types with the prominence of coa type X (16.6%) were identifed [34]. In a Sudanese study conducted by Ibrahim et al, resistance to methicillin was found to be 56% and coa type III as the predominant type was reported (55.5%) [35].…”