COAGULASE-NEGATIVE MUTANTS OF<i>STAPHYLOCOCCUS AUREUS</i>: GENETIC STUDIES

Korman, Ruth Z.

doi:10.1128/jb.86.3.363-369.1963

Cited by 31 publications

(24 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…on August 1, 2020 by guest http://jb.asm.org/ Downloaded from PENICILLINASE PLASMID OF S. EPIDERMIDIS logic activities (11). A similar pleiotropic effect involving coagulase activity and phage susceptibility has been reported for mannitol-negative mutants of S. aureus (8). In our laboratory we have so far been unable to obtain coagulaseand mannitol-negative mutants from the S. aureus strains investigated.…”

Section: Discussionsupporting

confidence: 54%

Polyfunctional Penicillinase Plasmid in Staphylococcus epidermidis : Bacteriophage Restriction and Modification Mutants

Schaefler¹

1972

J Bacteriol

View full text Add to dashboard Cite

Growth of multiply resistant Staphylococcus epidermidis BV strains at 45 C resulted in the independent elimination of tetracycline resistance, of kanamycin resistance coupled with oxacillin resistance, or of penicillinase activity. The pH optimum for the elimination of kanamycin and oxacillin resistance was 5.6, whereas that for elimination of penicillinase activity was 8.0. The genetic determinant for penicillinase activity was linked with the genetic determninants for the active uptake of mannitol and ,8-glucosides, ribose fermentation, and phospho ,B-glucosidase activity. The penicillinase linkage group also contained determinants for phage adsorption, restriction, and modification, and for growth factor requirements of still unknown nature. The same linkage group, which is apparently of extrachromosomal nature, was eliminated from several S. epidermidis BV strains. By selection for novobiocin resistance, deletion mutants affecting several loci of the penicillinase plasmid were isolated. The isolation of restriction-negative and modification-negative mutants which retained phage susceptibility allowed the investigation of restriction and modification phenomena. A preliminary deletion map of the polyfunctional penicillinase plasmid is proposed.

show abstract

Section: Discussionsupporting

confidence: 54%

Polyfunctional Penicillinase Plasmid in Staphylococcus epidermidis : Bacteriophage Restriction and Modification Mutants

Schaefler¹

1972

J Bacteriol

View full text Add to dashboard Cite

show abstract

“…aureus would lose all three of these characters although possible loss of two could occur. However, a mutant lacking coagulase, ability t o utilize mannitol, and other properties was isolated by Korman (1963) after extensive ultraviolet treatment. Also there are a number of reports of antibiotic-resistant strains that have lost coagulase and other properties (McVeigh & Hobdy, 1952;Massieu, 1960).…”

Section: Staphylococcus Aureus Rosenbachmentioning

confidence: 99%

Staphylococci and Their Classification

Baird‐Parker

1965

Annals of the New York Academy of Sciences

View full text Add to dashboard Cite

“…Our provisional experience with the polymyxin susceptibility test has convinced us that this test will make possible the correct identification of a number of S. aureus variants not detected by routinely per- (9,10).…”

Section: Discussionmentioning

confidence: 99%

“…A positive result in either a coagulase or a clumping factor test is essential in the routine identification of Staphylococcus auras. Gxigulasenegative S. aureus variants have been isolated occasionally (3, 4, 13) or induced in vitro by mutagenization ( 5 , 8,9,10,14). To identify coagulase-negative S. aureus in clinical specimens, a simple test is desirable to separate them from coagulase-negative species proper, such as Staphylococcus epidermidis and Staphylococcus saprophyti-Ordinary coagulase-positive S. aureus strains are characterized by small inhibition zones around polymyxin tablets or discs as opposed to the coagulase-negative species (7).…”

mentioning

confidence: 99%

RECOGNITION OF COAGULASE‐NEGATIVE STAPHYLOCOCCUS AUREUS STRAINS BY PRIMARY POLYMYXIN SUSCEPTIBILITY TESTING

Heltberg¹,

Bruun²

1984

Acta Pathologica Microbiologica Scandinavica Series B: Microbio

View full text Add to dashboard Cite

A prospective study evaluating the usefulness of polymyxin susceptibility testing in unveiling coagulase‐negative S. aureus strains was undertaken. During a six months study period, 14 staphylococcal isolates from four patients were initially found to lack coagulase activity and to be polymyxin resistant; in comparison, approximately 1500 ordinary, coagulase‐positive, polymyxin resistant S. aureus isolates were found during the same period. One isolate from each of the four patients together with a previously isolated coagulase‐negative S. aureus strain from our own collection were further characterized. Two of the strains turned out to show delayed coagulase activity on retesting, while the other three strains failed to produce clotting in any coagulase assay. Judged by thermostable nuclease activity, phage typing and biochemical profiles these three coagulase‐negative strains are bona fide S. aureus strains. The polymyxin test thus appears to be useful in disclosing S. aureus variants not identified as S. aureus by routinely performed coagulase assays.

show abstract

COAGULASE-NEGATIVE MUTANTS OFSTAPHYLOCOCCUS AUREUS: GENETIC STUDIES

Cited by 31 publications

References 21 publications

Polyfunctional Penicillinase Plasmid in Staphylococcus epidermidis : Bacteriophage Restriction and Modification Mutants

Polyfunctional Penicillinase Plasmid in Staphylococcus epidermidis : Bacteriophage Restriction and Modification Mutants

Staphylococci and Their Classification

RECOGNITION OF COAGULASE‐NEGATIVE STAPHYLOCOCCUS AUREUS STRAINS BY PRIMARY POLYMYXIN SUSCEPTIBILITY TESTING

Contact Info

Product

Resources

About