Coagulase production by staphylococci on a solid medium

Penfold, John B.

doi:10.1002/path.1700560214

Cited by 18 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Taylor and McDiarmid (18) used rabbit, sheep, and ox plasmas in the plate test. Numerous plating media have been prepared with human or rabbit plasmas (6,7,10,15,17). Duthie and Lorenz (6) reported that cow, sheep, dog, guinea-pig, and mouse plasmas exhibited a relative deficiency in coagulase-reacting factor (CRF), whereas the plasmas of man, monkey, horse, cat, pig, fowl, and rabbit contained substantially more CRF.…”

mentioning

confidence: 99%

Comparison of Animal Sera for Suitability in Coagulase Testing 1

Orth¹,

Chugg²,

Anderson³

1971

Applied Microbiology

View full text Add to dashboard Cite

The sera of several animals were examined for suitability in coagulase testing. The assay for coagulase-reacting factor (CRF) activities of the whole sera indicated the following relative concentrations of CRF: human > pig > rabbit > horse > bovine, chicken, and lamb. Human, pig, and rabbit sera had adequate amounts of CRF for coagulase testing. The plasmin activities of the different sera, arranged from the strongest to the weakest, were as follows: rabbit > human > lamb >horse > bovine, chicken, and pig. Fibrinolysis was observed when rabbit, human, lamb, or horse sera were incorporated into coagulase test agars. Pig serum was superior to the other sera for use in the plate test for coagulase since it had adequate amounts of CRF and the plasminogen-plasmin system was not activated by staphylokinase or staphylococcal Muller factor. Heparinized pig plasma was more suitable than citrated pig plasma since citrate interfered with the growth of Staphylococcus aureus, and the use of heparinized plasma prevented false-positive coagulase reactions due to citrate utilization.

show abstract

mentioning

confidence: 99%

Comparison of Animal Sera for Suitability in Coagulase Testing 1

Orth¹,

Chugg²,

Anderson³

1971

Applied Microbiology

View full text Add to dashboard Cite

show abstract

“…The pour plate method along with tube a n d slide tests are the most common procedures for testing the production of coagulase by S. aureus (Boothby et al 1979). Penfold (1944) a n d Reid a n d Jackson (1945) inoculated the surface of a medium containing plasma with culture of staphylococci. Colonies of coagulase-positive staphylococci were differentiated by the surrounding coagulation zone.…”

Section: Pour Plate Methodsmentioning

confidence: 99%

Significance of Staphylocoagulase in Food Microbiology: A Review

Ocasio

Fung

1984

Journal of Food Safety

View full text Add to dashboard Cite

Staphylocoagulase is an important enzyme for the identification of Staphylococcus aureus. It is also commonly related to pathogenicity of the organism. This review discusses the history of staphylocoagulase, its importance in the identification of staphylococci, its relationship with other important enzymes of staphylococci, and its role in food microbiology and food safety. Chemical properties, synthesis, mechanism of enzymatic activities, factors influencing detection, methods of detection, possible occurrence of the enzyme in foods, and its heat stability are also reviewed.

show abstract

“…aureus and Staph. albus in the clinical laboratory, since the whole plasma-containing solid media (Penfold, 1944;Lack, 1957;Esber and Faulconer, 1959) show false-positive reactions with coagulase-negative staphylococci (Williams and Harper, 1946;Klemperer and Haughton, 1957;Lotter and Horstman, 1967). These non-specific reactions appear practically eliminated in the medium of Klemperer and Haughton (1957) because of its low plasma content (3 %).…”

mentioning

confidence: 99%