Coat Protein Gene-Mediated Resistance to Soybean Mosaic Virus in Transgenic Soybean

Abstract

Somatic embryos of Glycine max (L.) Merrill cultivar 'Jack' were co-transformed with coat protein (CP) gene of attenuated isolates of soybean mosaic virus (SMV) and hygromycin phosphotransferase (hpt) gene by means of microprojectile bombardment. These transformed embryogenic tissues were selected in hygromycincontaining liquid medium. The hygromycin-resistant embryogenic tissues obtained through the selection were regenerated, and CP gene was detected in the 11 transgenic plants out of them. In order to assess their resistance to SMV, mechanical inoculation was performed in T 1 generation. The disease symptom was examined visually and confirmed by the enzyme-linked immunosorbent assay (ELISA). Finally we obtained three independent lines highly resistant to SMV. This is the first report of the soybean plants that were conferred a high resistance to SMV by the transformation with CP gene of the SMV attenuated isolates. In these three lines, the presence of transgene transcript was confirmed by Northern blot analysis, and the transgene product was detected in two of them by Western blot analysis.

Virus-induced gene silencing in soybean seeds and the emergence stage of soybean plants with Apple latent spherical virus vectors

“…However, transformation can be applied to only a few soybean cultivars and the efficiency of transformation is low. Furthermore, long time (more than 1 year) is needed to obtain transformed soybeans (Furutani 2007;Tougou 2009). Several reports also showed that co-suppression by overexpression of a transgene were reset during the reproduction in several transgenic plants, including soybean plants (Aida and Shibata 1998;Furutani et al 2007;Mitsuhara et al 2002;Yamagishi et al 2006b).…”

Gene Duplication and RNA Silencing in Soybean

“…The resistance did not need protein translated from the transgene [85][86][87], which led to the understanding that RNA is the factor that conferred resistance to the plants and that the enhanced resistance is acquired via a mechanism analogous to that involved in co-suppression. Using this strategy, soybean plants resistant to Soybean mosaic virus (SMV) [88][89][90], or Soybean dwarf virus [91,92] have been produced.…”

Genetic Engineering for Viral Disease Management in Plants

“…In another attempt by Furutani et al (2006), somatic embryos of Glycine max (L.) Merrill cultivar 'Jack' were co-transformed with coat protein (CP) gene of attenuated isolates of soybean mosaic virus (SMV) and hygromycin phosphotransferase (hpt) gene by means of microprojectile bombardment. These transformed embryogenic tissues were selected in hygromycin-containing liquid medium.…”