Coat protein of potyviruses 2. amino acid sequence of the coat protein of potato virus Y

SUMMARYThe sequence of the T-terminal 1611 nucleotides of the genome of the tobacco veinal necrosis strain of potato virus Y (PVY N) was determined. The sequence revealed an open reading frame of 1285 nucleotides, of which the start was not identified, and an untranslated region of 316 nucleotides upstream of a poly(A) tract.Comparison of the open reading frame with the amino-terminal sequence of the viral coat protein enabled mapping of the start of the coat protein at amino acid -267, and indicated that maturation of this protein requires proteolytic processing from a larger polyprotein precursor at a glutamine/glycine dipeptide sequence. The coat protein of PVY N displayed significant (51 to 63 %) sequence homology to the coat proteins of four other potyviruses, tobacco etch virus, tobacco vein mottling virus, plum pox virus and sugarcane mosaic virus. Even higher sequence homology (91%) was detected with the coat protein of a fifth potyvirus, pepper mottle virus (PeMV). This homology was of the same level as found between the coat proteins of PVY N and a second strain of this virus, PVY D. Since, moreover, pVyNand PeMV were the only potyviruses displaying homology in the 3'-terminal, non-translated regions of their genomes, we conclude that PeMV should be regarded as a strain of PVY.

Section: Short Communicationmentioning

confidence: 56%

Nucleotide Sequence of the 3'-terminal Region of Potato Virus YN RNA

Vlugt

Allefs

Haan

et al. 1989

“…The CP of the PPV-NAT isolate has a deletion of 15 amino acids near the N terminus. All PPV CPs are larger than those of other potyviruses like sugarcane mosaic virus (SCMV; Gough et al, 1987), pepper mottle virus (PeMV; Dougherty et al, 1985), TVMV (Domier et al, 1986), TEV (Allison et al, 1986) and potato virus Y (Shukla et al, 1986). The larger CP is reflected in the extended nucleotide sequence of PPV-NAT compared to TVMV and TEV.…”

Section: Qpppviqpaprttapmlnpiftpattqpatkpvsqvsgpqmentioning

confidence: 95%

The Complete Nucleotide Sequence of Plum Pox Virus RNA

Maiß¹,

Timpe²,

Brisske³

et al. 1989

209

143

SUMMARYThe complete nucleotide sequence of the RNA of an aphid non-transmissible plum pox virus (PPV-NAT) isolate has been determined from five overlapping cDNA clones, cDNA prepared by primer extension was used to determine the 5' terminus. The assembled RNA is 9741 nucleotides in length, excluding a 3' terminal poly(A) sequence. One large open reading frame starts at nucleotide positions 36 to 38 and is terminated with an UAG codon at positions 9522 to 9524. The putative start codon is located at positions 147 to 149. The encoded polyprotein has a predicted Mr of 353"8K. Comparison of cistrons from tobacco vein mottling virus and tobacco etch virus with those predicted for PPV-NAT indicated a similar genome organization. A highly conserved sequence of 12 nucleotides was found in the 5' non-coding region of these three potyviruses. The potential polyadenylation signal from yeast (UAUGU) was found in the 3' non-coding region of PPV-NAT and several other members of the potyvirus group.

“…2). We have previously commented (Shukla et al, 1986(Shukla et al, , 1988cShukla & Ward, 1988) on the close structural relationship between the coat proteins of PeMV and strains of PVY, and the possibility that PeMV is a strain of PVY. Comparison of the 3' untranslated region of PeMV (Dougherty et al, 1985) with that published for PVY-I (Rosner & Raccah, 1988) shows that the 3' untranslated sequences are 92% homologous (Table 1), supporting our contention that they are closely related genetically and should be considered strains of one virus.…”

Section: Discussionmentioning

confidence: 99%

The Use of 3' Non-coding Nucleotide Sequences in the Taxonomy of Potyviruses: Application to Watermelon Mosaic Virus 2 and Soybean Mosaic Virus-N

Frenkel

Ward

Shukla

1989

160

SUMMARYThe sequence of the 3' 1106 nucleotides of the watermelon mosaic virus 2 (WMV 2) genome has been determined. The sequence contains the complete coding region of the viral coat protein followed by a 3' untranslated sequence of 251 nucleotides. When these sequences were compared with the equivalent regions of the N strain of soybean mosaic virus (SMV-N), the coat protein coding regions were 82 ~ homologous, whereas the 3' untranslated sequences were 78~ homologous. Optimal alignment of the 3' untranslated regions of RNA from 13 strains of seven other distinct potyviruses revealed that the degree of homology between strains was in the range 83 to 99~. In contrast, the sequences from distinct viruses had identities in the range 39 to 53~, comparable to the level of identity found between the 3' non-coding regions of viruses from unrelated plant virus groups. On the basis of these results, WMV 2 and SMV-N could be regarded as strains of one virus. These results also suggest that the sequence of the 3' untranslated region of the potyvirus genome may be an accurate marker of genetic relatedness and could serve as an aid to identification and classification of potyviruses.