Coat protein of potyviruses 7. Amino acid sequence of peanut stripe virus

Abstract

The amino acid sequence of the 287-residue coat protein of peanut stripe virus (PStV) was determined from the sequences of overlapping peptide fragments. Results indicated that the amino terminus was blocked by an acetyl group, as has previously been found for the coat protein of Johnsongrass mosaic potyvirus. Comparison of the PStV sequence with coat proteins of 20 distinct potyviruses gave sequence identities of 47-57%, except for zucchini yellow mosaic virus (ZYMV), passionfruit woodiness virus (PWV), and the related strains watermelon mosaic virus 2 (WMV 2) and soybean mosaic virus-N, which showed sequence identities of 70-76%. Several amino acid residues which were common to the core sequences of these coat proteins were at positions previously found to be invariant among potyvirus coat proteins. The degree of these similarities suggests that although PStV, WMV 2, ZYMV, and PWV are distinct potyviruses, they share a common ancestor in their evolutionary development.

Identificação e caracterização de um potyvírus isolado de Zinnia elegans

“…Nela foram identificados: (1) o sítio de clivagem entre as proteínas NIb e CP [glutamina (Q)/glicina (G)] que é comum aos vírus pertencentes a família Potyviridae (Van der Vlugt et al, 1993); (2) o motivo DAG (4aa a partir do sítio de clivagem) que está relacionado à transmissão por afídeos e presente na região Nterminal da CP dos potyvírus assim transmitidos (Atreya et al,1990;Gal-On et al, 1992) A porcentagem de similaridade das seqüências da CP completa e do "core" tem sido utilizada para identificar espécies de potyvírus e traçar relações filogenéticas entre eles (Ward et al, 1992;Shukla et al, 1994). Comparações entre seqüências completas da CP de muitos membros do gênero Potyvirus revelaram que espécies distintas apresentam de 38 a 71% de similaridade e estirpes de um mesmo vírus têm de 90 a 99% (Ward et al, 1992), mas há várias exceções a esta regra (McKern et al, 1991;Tracy et al, 1992;Uyeda, 1992). Alguns trabalhos demonstraram que comparações da seqüência do "core" da CP são mais representativas .…”

Sequence data to settle the taxonomic position of bean common mosaic virus and blackeye cowpea mosaic virus isolates

“…Furthermore, both the sequence and size of the 3'-NTR of NL3 differ greatly from those of NL1, NY15 and W, indicating that NL3 should be considered a distinct virus. In a sequence comparison of complete coat proteins of NL1, NY15 and W with that of isolates of peanut stripe virus (serologically closely related to BCMV isolates) and BCMV strain NL4, a similarity of 92 to 95 % was found and their 3'-NTRs showed a similarity of more than 85 to 98 % (McKern et al, 1991;Vetten et al, 1992;Cassidy et al, 1993). Furthermore, NL3 showed high sequence similarities of 98 and 92% at the coat protein and Y-NTR levels, respectively, with the sequence of BCMV strain NL8 (Vetten et al, 1992).…”

Coat Protein Properties Suggest that Azuki Bean Mosaic Virus, Blackeye Cowpea Mosaic Virus, Peanut Stripe Virus, and Three Isolates from Soybean Are All Strains of the Same Potyvirus

“…Protein bands were stained with Coomassie blue R250. Electrophoresis was carried out in 10% polyacrylamide gels [17], Amino acid analysis of intact coat proteins or pep tide fragments was carried out by hydrolyzing samples at 110° in 5.8 M HCI containing0.01% phenol for20-22 h under N>. The samples were then vacuum dried and analyzed on a Waters amino acid analysis ion-exchange column.…”

“…Values in parentheses are those of homologous peptides from PStV coat protein [17], where these differ from the analyzed peptide. A homolo gous PStV peptide for peak J could not be identified.…”

“…Similarly, the final peak 22 was omitted from comparisons, being at least partly caused by the sharp gradient of acetonitrile used to wash the column of bound substances. Peptides contained in peaks 4-21 of PStV-Stripe A were subjected to amino acid analysis which allowed them to be placed with in the known amino acid sequence [17] of this coat protein (see figure 4], These peptides ac counted for most of the sequence, except for the insoluble core peptide (residues H8-176) and some hydrophilic peptides, the latter prob ably eluting in the isocratic peaks l-3. Retention time (min) Fig.…”