Coating of titanium implants with type‐I collagen

Abstract

Purpose: Type-I collagen, the major structural protein in bone, has beneficial properties regarding bone regeneration. Little is known about the potential effects of collagen coating on orthopedic implants. method^ 3 to 6 @cm2 of lyophilized type-I collagen was absorbed on titanium rods. Six coated and uncoated pins of 0.9 mm diameter were inserted into the tibia of adult male Wistar rats for 1, 2, 4, 7, 14, and 28 days. Specimens were embedded in methacrylate-based Technovit 9100N resin. From one portion cutting and grinding sections were obtained. The implant was removed from the other half that was depolymerized, sectioned, and mounted for immunohistochemistry.Results: At day 4, the interface around the collagen-coated implants displayed a granulation tissue with higher numbers of cathepsin D-positive mononucleated cells compared to the uncoated implants @ < 0.05). Active osteoblasts, reactive for osteopontin, were increased around the collagen-coated pins at day 4 and 7 (p < 0.01). After 28 days of implantation, direct bone contact averaged 74.9% around the collagen-coated implants and 62.1% around uncoated implants (NS). The amount of newly formed bone averaged 76.3% around the collagen-coated pins and 67.8% around the uncoated pins (NS). The histomorphometric findings were confirmed by SRpCT in two specimens. Conclusions:The earlier observation of mononuclear phagocytozing cells and the earlier and higher expression of bone-specific matrix proteins suggest an increased early bone remodeling around titanium pins through collagen coating. A tendency towards increased bone formation was observed around the coated implants.

Biochemical modification of titanium surfaces: Peptides and eCM proteins

“…If confirmed, these observations could open the way to finely-tuned BMTiS, where, depending on the specific site and application, a specific molecule or set of molecules could be used to its best effect. Rammelt et al (2004) presented a study in rat, involving coating by adsorption of type-I collagen on titanium rods. Coated and uncoated pins were inserted into the tibia of adult rats and histology and immunohistochemistry were performed at 1, 2, 4, 7, 14 and 28 days.…”

Bioactive nanocomposite coatings of collagen/hydroxyapatite on titanium substrates

“…Recent studies concerning collagen coatings on Ti hard-tissue implants have demonstrated their effective roles in stimulating cellular responses [11], increasing bone remodeling [12,13], and improving bone growth and bone-implant contact [14]. Therefore, hybrids of collagen and HA have greater potential for clinical applications than their pure HA or collagen equivalents, because they benefit from the advantageous properties of both materials, while retaining a similar composition and structure to that of human hard tissues.…”

Decellularization of Fibroblast Cell Sheets for Natural Extracellular Matrix Scaffold Preparation

“…47 Several ECM components, such as collagen, fibronectin, and GAGs, have shown the ability to control inflammatory responses. [48][49][50] The ECM scaffolds in our study maintained considerable amount of natural collagen I and fibronectin, which may exert immunomodulating effects in vivo. Different decellularization methods resulted in ECM scaffolds with different components and mechanical properties, which could be applied in different tissue regeneration applications.…”