Coaxial 3D bioprinting of self-assembled multicellular heterogeneous tumor fibers

Dai, Xingliang; Liu, Libiao; Ouyang, Jia; Li, Xinda; Zhang, Xinzhi; Lan, Qing; Xu, Tao

doi:10.1038/s41598-017-01581-y

Cited by 118 publications

(82 citation statements)

References 40 publications

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“…3D bioprinting is based on computer aided additive manufacturing technology to precisely control the distribution of biomaterials, cells and growth factors in 3D structure to maintain the bioactivity of cells . Compared with seeding cells on 3D scaffolds, 3D bioprinting can better control the position of biomaterials and cells, and promote cell–cell and cell‐ECM interactions . In this study, based on previously reported methods, gelatin, alginate and fibrinogen were chosen for bioink to construct glioma cell model.…”

Section: Discussionmentioning

confidence: 99%

“…26 Compared with seeding cells on 3D scaffolds, 3D bioprinting can better control the position of biomaterials and cells, and promote cell-cell and cell-ECM interactions. 18,27 In this study, based on previously reported methods, 22 gelatin, alginate and fibrinogen were chosen for bioink to construct glioma cell model. The viability of cell was 89.06 AE 3.58% after bioprinting.…”

Section: Discussionmentioning

confidence: 99%

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3D bioprinted glioma cell‐laden scaffolds enriching glioma stem cells via epithelial–mesenchymal transition

Wang

Dai

Zhang

et al. 2018

J Biomedical Materials Res

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Glioma stem cells (GSCs) are thought to be the root cause of tumor recurrence and drug resistance in glioma patients. In‐depth study of GSCs is of great significance for developing the treatment strategies of glioma. Unfortunately, it is difficult and takes complicated process to obtain GSCs. Therefore, establishing an ideal in vitro model for enriching GSCs will greatly promote the study of GSCs. In this study, the stemness properties of glioma cells were enhanced in three‐dimensional (3D) bioprinted tumor model. Furthermore, the possible molecular mechanism of GSCs enrichment: epithelial–mesenchymal transition (EMT) was explored. Compared with two‐dimensional cultured cells, the proportion of GSCs and EMT‐related genes in 3D cultured cells were significantly increased. Moreover, the 3D cultured glioma cells with improved stemness properties resulted in higher drug resistance in vitro and tumorigenicity in vivo. Taken together, 3D bioprinted glioma cell‐laden scaffold provides a proper platform for the enrichment of GSCs and it is expected to further promote the research on glioma drug resistance. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 383–391, 2019.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

3D bioprinted glioma cell‐laden scaffolds enriching glioma stem cells via epithelial–mesenchymal transition

Wang

Dai

Zhang

et al. 2018

J Biomedical Materials Res

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“…Grolman, Zhang, Smith, Moore, and Kilian () built a high‐throughput heterogeneous tumor model containing immune cells and tumor cells by extrusion‐based bioprinting (Figure a). Dai et al () extruded a coaxial 3D model containing glioma stem cells and human MSCs (Figure b). During the culturing period, glioma stem cells fused with MSCs and induced higher gene expression compared with 2D culture models.…”

Section: Techniques For Constructing Heterogeneous Tumor Modelsmentioning

confidence: 99%

“…(b) Coaxial 3D bioprinting cell fibers containing RFP‐expressing tumor cells and GFP‐expressing MSCs. From Dai et al (), copyright© Dai et al CC‐BY 4.0. (c) Construction and characterization of a high‐throughput cell‐patterning platform for printing in a 3D in vitro ovarian cancer co‐culture model.…”

Section: Techniques For Constructing Heterogeneous Tumor Modelsmentioning

confidence: 99%

Review on biofabrication and applications of heterogeneous tumor models

Liu

Yao

Pang

et al. 2019

J Tissue Eng Regen Med

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Resolving the origin and development of tumor heterogeneity has proven to be a crucial challenge in cancer research. In vitro tumor models have been widely used for both scientific and clinical research. Currently, tumor models based on 2D cell culture, animal models, and 3D cell‐laden constructs are widely used. Heterogeneous tumor models, which consist of more than one cell type and mimic cell–cell as well as cell–matrix interactions, are attracting increasing attention. Heterogeneous tumor models can serve as pathological models to study the microenvironment and tumor development such as tumorigenesis, invasiveness, and malignancy. They also provide disease models for drug screening and personalized therapy. In this review, the current techniques, models, and oncological applications regarding 3D heterogeneous tumor models are summarized and discussed.

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“…To date, the fabrication of thick 3D constructs (see Figure 2. 2 (a) and (b)) [47,49], and vascularized tissues [50][51][52] (see Figure 2.2 (c)) has been achieved using extrusion printing. Gao et al, [50] reported a strategy to fabricate large-scale organs with built-in microchannels, as shown in…”

Section: Extrusion Printingmentioning

confidence: 99%

Study of hydrogels for 3D printing of constructs with strong interfacial bonding

Li¹

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Hydrogels are the most appealing candidates of biomaterials for bioprinting. In this field of bioprinting, the lack of suitable hydrogels remains a major challenge. Thus, choosing appropriate hydrogels is the key to successfully print self-supporting 3D constructs. Most importantly, the design criteria regarding the bioinks and the obtained constructs should be made clear in advance. Therefore, the first task of this study is to clarify the design criteria regarding the important properties of a potential bioink and the generated 3D construct, including rheological, interfacial, structural, biological, and degradation properties, which are crucial for printing of complex and functional 3D structures. A method is developed for evaluating the printability of a candidate hydrogel through simulating its rheological behaviors before, during, and after printing. After that, two novel strategies are proposed in order to obtain multilayered hydrogel constructs with strong interface bonding. In the first strategy, trisodium citrate (TSC) acts as a chelating agent to remove the superficial Ca 2+ at each layer. The subsequent post-crosslinking of constructs in a calcium chloride bath will further create the crosslinks and enhance the adhesion between adjacent layers. The second strategy for improving the adhesion between printed layers of a construct is to exploit the interaction between two oppositely charged hydrogels. On the basis of these two strategies, the exciting results have been obtained, which include strong interfacial bonding between two layers of the printed structures, good shape fidelity Even though I have put in great effort for completion of this thesis, it would not have been possible without the support and help of many individuals and organizations. I would like to extend my sincerest thanks to all of them. Firstly, I would like to express my gratitude to my supervisor, Prof. Lin Li for giving me an opportunity to be his student. His valuable guidance, warm encouragements motivated me to conduct experiments, write papers and complete my thesis. I have benefitted much from his rigorous attitude towards scientific research. Special thanks are given to my lab mate and close friend, Dr. Yu Jun Tan, for her assistance, knowledge sharing and valuable discussion. I also appreciate my group member Dr. Sijun Liu, who provides his assistance during my PhD study. My appreciation also goes to the other two group members, Mr Anil Kumar Bastola and Miss Ying Zhen Low, for their encouragement and accompany.

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Coaxial 3D bioprinting of self-assembled multicellular heterogeneous tumor fibers

Cited by 118 publications

References 40 publications

3D bioprinted glioma cell‐laden scaffolds enriching glioma stem cells via epithelial–mesenchymal transition

3D bioprinted glioma cell‐laden scaffolds enriching glioma stem cells via epithelial–mesenchymal transition

Review on biofabrication and applications of heterogeneous tumor models

Study of hydrogels for 3D printing of constructs with strong interfacial bonding

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