Coaxial Electrohydrodynamic Spraying: A Novel One-Step Technique To Prepare Oligodeoxynucleotide Encapsulated Lipoplex Nanoparticles

Abstract

This study investigates coaxial electrohydrodynamic spraying (electrospray for short) as a novel, rapid, real time and single-step method to produce oligodeoxynucleotide (ODN) encapsulated lipoplex nanoparticles for either intravenous injection or, potentially, pulmonary delivery. Using a coaxial needle setup, we produced G3139 (oblimerson sodium, or Genasense) encapsulated lipoplex nanoparticles, and investigated the effects of production parameters on nanoparticle size and structure. Careful control of production parameters yielded lipoplex nanoparticles 190 +/- 39 nm in diameter with unilamellar structure and 90 +/- 6% encapsulation efficiency of G3139. Both nontargeted and transferrin-targeted G3139 lipoplex nanoparticles were efficiently delivered to K562 cells and downregulated the bcl-2 protein expression by 34 +/- 6% and 57 +/- 3% respectively.

Preparation, Characterization, and Optimization of Folic Acid-Chitosan-Methotrexate Core-Shell Nanoparticles by Box-Behnken Design for Tumor-Targeted Drug Delivery

“…These factors include effect of solvent properties (concentration, conductivity, surface tension, stability of the electrospray behavior, and interfacial surface tension between the inner and outer liquids), effect of applied voltage, needle diameters of core and shell solutions, ratio of flow rates between the core and shell solutions, and the distance between the nozzle and aluminum foil in which the latter one has already been studied (41,44,45,(51)(52)(53). Three independent factors like concentration, applied voltage, and ratio of flow rates between the core and shell solutions which can have the greatest impact on particle size were selected and studied further.…”

“…The coaxial liquid beams drop out from Taylor cone vertex, and they convert to capsules or multilayered drops. Electrospray is a direct and single-step method for the preparation of solid nanoparticles and also can be done with mild process conditions (41)(42)(43)(44)(45).…”

Biological Events Occuring on the Biosis–Abiosis Interface: Cellular Responses Induced by Implantable Electrospun Nanofibrous Scaffolds

“…The eventual jet, by which the outer polymeric solution encapsulates the inner proteinaceous liquid, breaks into droplets to generate microparticles with core-shell structure. This technique is preferred for preparing protein-loaded microcapsules, because it totally eliminates the emulsion step that is basically unsuitable for sensitive biomacromolecules [25,26]. Coaxial electrospray is envisioned a promising approach to prepare biomacromolecule-loaded microcapsules for controlled drug delivery applications.…”

Micro/nano-encapsulation of a phase change material by coaxial electrospray method

“…Moreover, several research groups such as Lee et al [23], Wu et al [24], Zhang et al [25], Lee et al [26], Yu et al [27], and Luo et al [28] worked with coaxial electrospray and did not mention the position of the inner needle tip relative to that of the outer needle. However, the core capillary protruding outside the shell capillary is dependent on the feeding rates of the inner and the outer liquids, the interfacial tension, and the viscosity ratio of the liquids [17,29].…”