The injection of a conducting solution (i.e., NS) rendered the current that was previously sufficient to elicit a motor response (0.5 mA) ineffective. The most likely reason for this change is that the conductive area surrounding the stimulating needle expanded after the injection and dispersion of the conducting solution (i.e., NS), thereby reducing the current density at the target nerve. This effect can be reversed by injecting a nonconducting solution (i.e., D5W) via the stimulating needle.