Cobalamin (vitamin B12) positively regulates interleukin-6 levels in rat cerebrospinal fluid

Scalabrino, Giuseppe; Corsi, Massimiliano M.; Veber, Daniela; Buccellato, Francesca R.; Pravettoni, Gabriella; Manfridi, Alfredo; Magni, Paolo

doi:10.1016/s0165-5728(02)00095-4

Cited by 35 publications

(30 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It helps maintaining normal functions of the brain. vit B 12 deficient neuropathy is well established in humans, and has also been described in animal models [19]. The CNS and especially the spinal cord are severely damaged by vit B 12 deficiency [20].…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Neuropathological and neuroprotective features of vitamin B12 on the dorsal spinal ganglion of rats after the experimental crush of sciatic nerve: an experimental study

et al. 2013

View full text Add to dashboard Cite

BackgroundSpinal motoneuron neuroprotection by vitaminB12 was previously reported; the present study was carried out to evaluate neuroprotectivity in the dorsal root ganglion sensory neuron.MethodsIn present study thirty-six Wister-Albino rats (aged 8–9 weeks and weighing 200–250 g) were tested. The animals were randomly divided into 6 groups which every group contained 6 rats. Group A: received normal saline (for 42 days); Group B: vitamin B12 was administered (0.5 mg/kg/day for 21 days); Group C: received vitamin B12 (1 mg/kg/day for 21days); Group D: received vitamin B12 (0.5 mg/kg/day for 42 days); Group E; received vitamin B12 (1 mg/kg/day for 42 days); Group F; received no treatment. The L5 Dorsal Root Ganglion (DRG) neurons count compared to the number of left and right neurons .Furthermore, DRG sensory neurons for regeneration were evaluated 21 or 42 days after injury (each group was analyzed by One-Way ANOVA test).Results(1): The comparison of left crushed neurons (LCN) number with right non-crushed neurons in all experimental groups (B, C, D and C), indicating a significant decline in their neurons enumeration (p<0/05). (2): The comparison of test group’s LCN with the control group’s LCN revealed a significant rise in the number of experimental group neurons (p<0/05). (3): Moreover, comparing the number of right neurons in experimental groups with the number of neurons in crushed neurons indicated that the average number of right neurons showed a significant increase in experimental groups (p<0/05).ConclusionConsequently, the probability of nerve regeneration will be increased by the increment of the administered drug dosage and duration. On the other hand, the regeneration and healing in Dorsal Spinal Ganglion will be improved by increase of administration time and vitamin B12 dose, indicating that such vitamin was able to progress recovery process of peripheral nerves damage in experimental rats. Finally, our results have important implications for elucidating the mechanisms of nerve regeneration. Moreover, the results showed that vitaminB12 had a proliferative effect on the dorsal root ganglion sensory neuron.Virtual slidesThe virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/7395141841009256

show abstract

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Neuropathological and neuroprotective features of vitamin B12 on the dorsal spinal ganglion of rats after the experimental crush of sciatic nerve: an experimental study

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Direct measurement of methylation, such as by using [ 14 C]ethanolamine, in the B 12 -deficient fruit bat has shown that there is no decrease in methylation of lipids or proteins, and no change in the myelin membrane protein profile in comparison to controls [12,[46][47][48]. The same results were demonstrated in an article by Deacon et al, titled Vitamin B 12 neuropathy is not due to failure to methylate myelin basic protein.…”

Section: The Metb 12 -Mtr Hypothesismentioning

confidence: 69%

Nitrous oxide-induced B12 deficiency myelopathy: Perspectives on the clinical biochemistry of vitamin B12

Hathout

El‐Saden

2011

Journal of the Neurological Sciences

View full text Add to dashboard Cite

“…Interesting homeostatic links between Cbl and pivotal cytokines are also emerging, indicative of complex but still incompletely defined regulatory circuits: MeCbl lowers interleukin-6 (IL-6) expression in peripheral blood monocytes [12], whilst Cbl deficiency raises circulating IL-6 in humans [13] and Cbl physiological status regulates IL-6 levels in rat cerebrospinal fluid [14]. Moreover, in both rodents and humans there appears to be an inverse relation between Cbl physiological levels and tumour necrosis factor alpha (TNF- α ) serum levels [15].…”

Section: Introductionmentioning

confidence: 99%

Biphasic Modulation of NOS Expression, Protein and Nitrite Products by Hydroxocobalamin Underlies Its Protective Effect in Endotoxemic Shock: Downstream Regulation of COX-2, IL-1β, TNF-α, IL-6, and HMGB1 Expression

Sampaio

Dalli

Brancaleone

et al. 2013

Mediators of Inflammation

View full text Add to dashboard Cite

Background. NOS/•NO inhibitors are potential therapeutics for sepsis, yet they increase clinical mortality. However, there has been no in vivo investigation of the (in vitro) •NO scavenger, cobalamin's (Cbl) endogenous effects on NOS/•NO/inflammatory mediators during the immune response to sepsis. Methods. We used quantitative polymerase chain reaction (qPCR), ELISA, Western blot, and NOS Griess assays, in a C57BL/6 mouse, acute endotoxaemia model. Results. During the immune response, pro-inflammatory phase, parenteral hydroxocobalamin (HOCbl) treatment partially inhibits hepatic, but not lung, iNOS mRNA and promotes lung eNOS mRNA, but attenuates the LPS hepatic rise in eNOS mRNA, whilst paradoxically promoting high iNOS/eNOS protein translation, but relatively moderate •NO production. HOCbl/NOS/•NO regulation is reciprocally associated with lower 4 h expression of TNF-α, IL-1β, COX-2, and lower circulating TNF-α, but not IL-6. In resolution, 24 h after LPS, HOCbl completely abrogates a major late mediator of sepsis mortality, high mobility group box 1 (HMGB1) mRNA, inhibits iNOS mRNA, and attenuates LPS-induced hepatic inhibition of eNOS mRNA, whilst showing increased, but still moderate, NOS activity, relative to LPS only. experiments (LPS+D-Galactosamine) HOCbl afforded significant, dose-dependent protection in mice Conclusions. HOCbl produces a complex, time- and organ-dependent, selective regulation of NOS/•NO during endotoxaemia, corollary regulation of downstream inflammatory mediators, and increased survival. This merits clinical evaluation.

show abstract

Cobalamin (vitamin B12) positively regulates interleukin-6 levels in rat cerebrospinal fluid

Cited by 35 publications

References 66 publications

RETRACTED ARTICLE: Neuropathological and neuroprotective features of vitamin B12 on the dorsal spinal ganglion of rats after the experimental crush of sciatic nerve: an experimental study

RETRACTED ARTICLE: Neuropathological and neuroprotective features of vitamin B12 on the dorsal spinal ganglion of rats after the experimental crush of sciatic nerve: an experimental study

Nitrous oxide-induced B12 deficiency myelopathy: Perspectives on the clinical biochemistry of vitamin B12

Biphasic Modulation of NOS Expression, Protein and Nitrite Products by Hydroxocobalamin Underlies Its Protective Effect in Endotoxemic Shock: Downstream Regulation of COX-2, IL-1β, TNF-α, IL-6, and HMGB1 Expression

Contact Info

Product

Resources

About