Cobalt-chromium-molybdenum but not titanium-6aluminium-4vanadium alloy discs inhibit human t cell activation in vitro

Faleiro, C.; Godinho, Inês; Reus, U.; Sousa, Maria de

doi:10.1007/bf00140600

Cited by 22 publications

(15 citation statements)

References 17 publications

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“…The effect was concentration dependent and increasing amounts of CoCl 2 correlated with a decrease in metabolic activity until reaching a very low plateau at 250 μ M CoCl 2 . These findings coincide with previous publications reporting an increased toxicity of Co ions on activated T‐lymphocytes including the inhibition of T‐cell activation in the presence of CoCr . Therefore, we analyzed the release of cytokines related to T‐cell activation in MNC cultured directly on CoCr, mCoCr discs, or with CoCl 2 (Fig.…”

Section: Discussionsupporting

confidence: 88%

Cytocompatibility of nitrogen plasma ion immersed medical cobalt–chromium alloys

Schröck

Schneider

Lutz

et al. 2013

J Biomedical Materials Res

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Wear particles and ion release from medical CoCr contribute to the risk for aseptic loosening. Nitrogen plasma immersion ion implantation (PIII) has been shown to reduce wear of CoCr but is associated with increased Co ion release. This work investigated the cytocompatibility of CoCr modified by nitrogen PIII at different temperatures (mCoCr). The osteosarcoma cell line Saos-2, mesenchymal stem cells (MSCs), and mononuclear cells (MNCs) were grown directly on CoCr/mCoCr discs or treated with CoCl2. Proliferation and metabolic activity of Saos-2 and MSC were decreased on mCoCr in correlation with increasing implantation temperature. The elevated Co ion release seemed to play a decisive role since analog effects were observed by treatment of cells with CoCl2. Proliferation of phytohemagglutinin-stimulated MNC was reduced in the presence of CoCr discs or CoCl2. For MNC-alloy cultures we observed an increase in interleukin 2 (IL-2) and a decrease in interferon γ (IFN-γ) and IL-10 secretion compared to cultures without alloys. The results of this study demonstrate that PIII process temperature and corresponding Co ion release correlate with material cytocompatibility. Therefore, the two competing parameters, reduction of wear and increase in Co ions, have to be taken into consideration for the evaluation of the clinical applicability of nitrogen-implanted CoCr.

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Section: Discussionsupporting

confidence: 88%

Cytocompatibility of nitrogen plasma ion immersed medical cobalt–chromium alloys

Schröck

Schneider

Lutz

et al. 2013

J Biomedical Materials Res

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“…Reduced cell number on CoCrMo may reflect a slower rate of proliferation, reduced cell survival on CoCrMo, or both. The biocompatibility of Ti-6Al-4V is considered by most groups to be superior to CoCrMo [32], and adverse effects of cobalt [33], chromium [34], and molybdenum [35] on other cell types have been identified. CoCrMo alloy particles have been shown to be toxic to some degree, with cell death increasing as the particle dosage increases [36,37].…”

Section: Days In Culturementioning

confidence: 99%

Mesenchymal stem cell interaction with ultra-smooth nanostructured diamond for wear-resistant orthopaedic implants

et al. 2008

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Ultra-smooth nanostructured diamond (USND) can be applied to greatly increase the wear resistance of orthopaedic implants over conventional designs. Herein we describe surface modification techniques and cytocompatibility studies performed on this new material. We report that hydrogen (H)-terminated USND surfaces supported robust mesenchymal stem cell (MSC) adhesion and survival, while oxygen- (O) and fluorine (F)-terminated surfaces resisted cell adhesion, indicating that USND can be modified to either promote or prevent cell/biomaterial interactions. Given the favorable cell response to H-terminated USND, this material was further compared with two commonly used biocompatible metals, titanium alloy (Ti-6Al-4V) and cobalt chrome (CoCrMo). MSC adhesion and proliferation were significantly improved on USND compared with CoCrMo, although cell adhesion was greatest on Ti-6Al-4V. Comparable amounts of the pro-adhesive protein, fibronectin, were deposited from serum on the three substrates. Finally, MSCs were induced to undergo osteoblastic differentiation on the three materials, and deposition of a mineralized matrix was quantified. Similar amounts of mineral were deposited onto USND and CoCrMo, whereas mineral deposition was slightly higher on Ti-6Al-4V. When coupled with recently published wear studies, these in vitro results suggest that USND has the potential to reduce debris particle release from orthopaedic implants without compromising osseointegration.

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“…Cr(VI) shows a biphasic pattern, with a stimulatory effect at the lowest concentrations tested and an inhibitory effect at higher concentrations. Faleiro et al [7] described the effect of cobalt–chromium–molybdenum (CoCrMo) disc samples on the CD3‐mediated in vitro response of human peripheral blood T lymphocytes. Inhibition of lymphocyte proliferation is observed in the presence of CoCrMo disc samples.…”

Section: Effects Of Chromium On Lymphocytesmentioning

confidence: 99%

Effects of chromium on the immune system

Shrivastava¹,

Upreti²,

Seth³

et al. 2002

FEMS Immunology &Amp; Medical Microbiology

305

130

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Chromium is a naturally occurring heavy metal found commonly in the environment in trivalent, Cr(III), and hexavalent, Cr(VI), forms. Cr(VI) compounds have been declared as a potent occupational carcinogen among workers in chrome plating, stainless steel, and pigment industries. The reduction of Cr(VI) to Cr(III) results in the formation of reactive intermediates that together with oxidative stress oxidative tissue damage and a cascade of cellular events including modulation of apoptosis regulatory gene p53, contribute to the cytotoxicity, genotoxicity and carcinogenicity of Cr(VI)-containing compounds. On the other hand, chromium is an essential nutrient required to promote the action of insulin in body tissues so that the body can use sugars, proteins and fats. Chromium is of significant importance in altering the immune response by immunostimulatory or immunosuppressive processes as shown by its effects on T and B lymphocytes, macrophages, cytokine production and the immune response that may induce hypersensitivity reactions. This review gives an overview of the effects of chromium on the immune system of the body.

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Cobalt-chromium-molybdenum but not titanium-6aluminium-4vanadium alloy discs inhibit human t cell activation in vitro

Cited by 22 publications

References 17 publications

Cytocompatibility of nitrogen plasma ion immersed medical cobalt–chromium alloys

Cytocompatibility of nitrogen plasma ion immersed medical cobalt–chromium alloys

Mesenchymal stem cell interaction with ultra-smooth nanostructured diamond for wear-resistant orthopaedic implants

Effects of chromium on the immune system

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