Cobalt doped proangiogenic hydroxyapatite for bone tissue engineering application

Kulanthaivel, Senthilguru; Roy, Bibhas; Agarwal, Tarun; Giri, Supratim; Pramanik, Krishna; Pal, Kunal; Ray, Sirsendu Sekhar; Maiti, Tapas K.; Banerjee, Indranil

doi:10.1016/j.msec.2015.08.052

Cited by 120 publications

(43 citation statements)

References 85 publications

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“…Besides organic components, metal ions in low doses are increasingly applied for the modification of bone graft materials to improve osteogenesis and neovascularization [ 1 – 3 ]. Co 2+ doping of calcium phosphate was shown to have proangiogenic effects [ 4 , 5 ]. Cu 2+ modification of a calcium phosphate ceramic resulted in an enhanced and directed vascularization [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Osteoclastic differentiation and resorption is modulated by bioactive metal ions Co2+, Cu2+ and Cr3+ incorporated into calcium phosphate bone cements

et al. 2017

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Biologically active metal ions in low doses have the potential to accelerate bone defect healing. For successful remodelling the interaction of bone graft materials with both bone-forming osteoblasts and bone resorbing osteoclasts is crucial. In the present study brushite forming calcium phosphate cements (CPC) were doped with Co2+, Cu2+ and Cr3+ and the influence of these materials on osteoclast differentiation and activity was examined. Human osteoclasts were differentiated from human peripheral blood mononuclear cells (PBMC) both on the surface and in indirect contact to the materials on dentin discs. Release of calcium, phosphate and bioactive metal ions was determined using ICP-MS both in the presence and absence of the cells. While Co2+ and Cu2+ showed a burst release, Cr3+ was released steadily at very low concentrations (below 1 μM) and both calcium and phosphate release of the cements was considerably changed in the Cr3+ modified samples. Direct cultivation of PBMC/osteoclasts on Co2+ cements showed lower attached cell number compared to the reference but high activity of osteoclast specific enzymes tartrate resistant acid phosphatase (TRAP), carbonic anhydrase II (CAII) and cathepsin K (CTSK) and significantly increased gene expression of vitronectin receptor. Indirect cultivation with diluted Co2+ cement extracts revealed highest resorbed area compared to all other modifications and the reference. Cu2+ cements had cytotoxic effect on PBMC/osteoclasts during direct cultivation, while indirect cultivation with diluted extracts from Cu2+ cements did not provoke cytotoxic effects but a strictly inhibited resorption. Cr3+ doped cements did not show cytotoxic effects at all. Gene expression and enzyme activity of CTSK was significantly increased in direct culture. Indirect cultivation with Cr3+ doped cements revealed significantly higher resorbed area compared to the reference. In conclusion Cr3+ doped calcium phosphate cements are an innovative cement modification because of their high cytocompatibility and support of active resorption by osteoclasts.

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Section: Introductionmentioning

confidence: 99%

Osteoclastic differentiation and resorption is modulated by bioactive metal ions Co2+, Cu2+ and Cr3+ incorporated into calcium phosphate bone cements

et al. 2017

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“…GO is a nano-sized particle with excellent dispersion properties in water and ethanol 42, and thus GO can be mixed into a MBG solution homogeneously. In addition, comparing with other metal elements, such as copper and cobalt, which are beneficial to bone formation 43, 44. The chemical structure of GO contains an abundance of hydroxyl, epoxy and carboxyl groups on the basal planes of the GO sheets, and GO therefore possesses large surface area and enhances cells adhesion 16.…”

Section: Discussionmentioning

confidence: 99%

Mesoporous bioactive glass combined with graphene oxide scaffolds for bone repair

Wang¹,

Liu²,

Yang³

et al. 2019

Int. J. Biol. Sci.

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Recently there has been an increasing interest in bioactive factors with robust osteogenic ability and angiogenesis function to repair bone defects. However, previously tested factors have not achieved satisfactory results due to low loading doses and a short protein half-life. Finding a validated stable substitute for these growth factors and apply it to the construction of porous scaffolds with the dual function of osteogenesis and angiogenesis is therefore vital for bone tissue regeneration engineering. Graphene oxide (GO) has attracted increasing attention due to its good biocompatibility, osteogenic, and angiogenic functions. This study aims to design a scaffold composed of mesoporous bioactive glasses (MBG) and GO to investigate whether the composite porous scaffold promotes local angiogenesis and bone healing. Our in vitro studies demonstrate that the MBG-GO scaffolds have better cytocompatibility and higher osteogenesis differentiation ability with rat bone marrow mesenchymal stem cells (rBMSCs) than the purely MBG scaffold. Moreover, MBG-GO scaffolds promote vascular ingrowth and, importantly, enhance bone repair at the defect site in a rat cranial defect model. The new bone was fully integrated not only with the periphery but also with the center of the scaffold. From these results, it is believed that the MBG-GO scaffolds possess excellent osteogenic-angiogenic properties which will make them appealing candidates for repairing bone defects. The novelty of this research is to provide a new material to treat bone defects in the clinic.

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“…The characteristic peak of ν 1 (PO 4 ) band was found at 963cm -1 . The mode ν 1 (PO 4 ) was associated to the total symmetric stretching mode of free tetrahedral phosphate ions [16]. The peak was observed at 1045cm -1 which correspond to asymmetric stretching mode of ν 3 (PO 4 ).…”

Section: Raman Analysismentioning

confidence: 94%

Cobalt Ions Doped Bioactive Ceramics for Biosensor Biomedical Applications

Kurinjinathan¹,

Arul²,

Ramya³

2018

IJCRR

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Cobalt ions doped hydroxyapatite (Co-HAp) was synthesized by ultrasonication assisted co-precipitation route. The phase of HAp was confirmed by XRD analysis. The crystallite size of doped sample was reduced (28%). The functional groupsof HAp were confirmed by FTIR and Raman analysis. Cobalt ions doped samples were showed magnetic behavior. In addition, optical properties of the samples were also carried out. Hence, cobalt ions doped samples could be an excellent candidate in biosensor and biomedical fields.

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Cobalt doped proangiogenic hydroxyapatite for bone tissue engineering application

Cited by 120 publications

References 85 publications

Osteoclastic differentiation and resorption is modulated by bioactive metal ions Co2+, Cu2+ and Cr3+ incorporated into calcium phosphate bone cements

Osteoclastic differentiation and resorption is modulated by bioactive metal ions Co2+, Cu2+ and Cr3+ incorporated into calcium phosphate bone cements

Mesoporous bioactive glass combined with graphene oxide scaffolds for bone repair

Cobalt Ions Doped Bioactive Ceramics for Biosensor Biomedical Applications

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