Cobalt (II) complex with novel unsymmetrical tetradentate Schiff base (ON) ligand: <i>in vitro</i> cytotoxicity studies of complex, interaction with DNA/protein, molecular docking studies, and antibacterial activity

Shokohi-pour, Zahra; Chiniforoshan, Hossein; Rahimmalek, Mehdi; Esmaeili, Seyed‐Alireza; Momtazi‐Borojeni, Amir Abbas

doi:10.1080/07391102.2017.1287006

Cited by 41 publications

(10 citation statements)

References 99 publications

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“…In UV–Vis spectrophotometry, the spectral changes were observed as hypochromic coupled red shift due to the intercalative binding between the aromatic chromophore of the ligand and base pairs of DNA, and as hyperchromism with concomitant blue shift due to electrostatic interactions through groove binding. [ 30 ] The absorption spectra of compounds 1 , 3 , and 4 exhibited absorption maxima centered at wavelength 405 nm, 400 nm, and 395 nm, respectively which may be assigned to the ligand‐to‐metal charge transfer (LMCT) [ 12,31 ] as seen in Figure 2(a–c). The hypochromic effect displayed by compounds with an increase in concentrations of CT‐DNA (Figure 2a–c) indicated the occurrence of classic intercalative binding mode.…”

Section: Resultsmentioning

confidence: 99%

Glutamine conjugated organotin(IV) Schiff base compounds: Synthesis, structure, and anticancer properties

Sharma

Agnihotri

Kumar

et al. 2021

Applied Organom Chemis

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Herein, glutamine conjugated organotin(IV) Schiff base compounds, [(SBGlu‐Naph)Sn (Me)2] (1), [(SBGlu‐Naph)Sn(n‐Bu)2] (2), [(SBGlu‐Sali)Sn (Me)2] (3), [(SBGlu‐Sali)Sn(n‐Bu)2] (4) and [(SBGlu‐Sali)Sn (Ph)2] (5), were synthesized by one‐pot reaction with an aldehyde (2‐hydroxy‐1‐naphthaldehyde) for 1 and 2, and 5‐methyl‐2‐hydroxybenzaldehyde (for 3–5) and respective diorganotin oxide. The compounds were characterized using elemental micro‐analyses, spectroscopic techniques (FT‐IR, 1H NMR, 13C NMR, and 119Sn NMR), mass spectrometry, and single‐crystal X‐ray diffraction analyses. Based on in‐silico ADME (absorption, distribution, metabolism, and excretion) and drug‐likeness properties, compounds 1, 3, and 4 were selected to investigate their DNA/Protein binding properties with calf thymus DNA (CT‐DNA) and bovine serum albumin (BSA) using spectrophotometry and spectrofluorimetry. The intercalative mode of binding with CT‐DNA was supported by molecular docking simulations. The emission spectral data of compounds with CT‐DNA showed 1 as a groove binder, and 3 and 4 as an intercalator which was confirmed by competitive displacement assays. Similarly, static and dynamic mode of interaction between the compounds and BSA was found. Furthermore, these compounds were screened for their cytotoxic activity against two human cancer cell lines; PC‐3 (Prostate) and Mg‐63 (Osteosarcoma) at different concentrations. Quantitative structure–activity relationship (QSAR)‐based regression models were developed and implemented on compounds 1, 3, and 4 that inferred compound 3 to be the most potential candidate for further in vivo studies.

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Section: Resultsmentioning

confidence: 99%

Glutamine conjugated organotin(IV) Schiff base compounds: Synthesis, structure, and anticancer properties

Sharma

Agnihotri

Kumar

et al. 2021

Applied Organom Chemis

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“…The ability of the drugs to induce either apoptosis or necrosis seems to be a primary factor in determining their anti‐cancer efficacy . The cytotoxicity of the three complexes and their ligands were determined in terms of a colorimetric microculture assay (MTT) in HeLa and KB cells, yielding IC 50 values shown in Table , which also shows the comparison of experimental conditions with the results of cisplatin experiments.…”

Section: Resultsmentioning

confidence: 99%

Structure and cytotoxicity of zinc (II) and cobalt (II) complexes based on 1,3,5‐tris(1‐imidazolyl) benzene

Zhu

Zhao

Peng

et al. 2019

Applied Organom Chemis

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Three novel complexes, [Zn (tib)2·(H2O)2]·(NO3)2 (1), [Co (tib)2]·2NO3 (2) and [Co2(tib)2(btc)]·H2O (3) [H4btc = 1,2,4,5‐benzenetetracarboxylic acid; H2tib = 1,3,5‐tris(1‐imidazolyl)benzene], were synthesized and characterized by single‐crystal X‐ray, IR and elemental analysis. The interaction of these complexes with FS‐DNA (fish sperm DNA) was monitored, and binding constants were determined using UV/Vis, which revealed that they have the ability to bind to FS‐DNA. DNA‐binding constants (K) for the three complexes were 2.2 × 104 m−1, 0.7 × 104 m−1 and 0.09 × 104 m−1, respectively. The interaction capacity of the complexes with FS‐DNA has been investigated by fluorescence spectroscopy. Stern–Volmer quenching plot values for complexes 1, 2 and 3 were 0.3784, 0.1028 and 0.076, respectively. The viscosity measurement suggested that complexes 1, 2 and 3 interact with DNA in an intercalation mode. In addition, anti‐cancer activities of these complexes investigated through MTT assays in vitro indicated that the complexes showed good cytotoxic activity against cancer cell lines. Cytotoxic activity of test complexes against two different cancer cell lines (HeLa and KB cells) showed significant cancer cell inhibition rates. Flow cytometry experiments and morphological apoptosis studies showed that the complexes induced apoptosis of HeLa tumor cell lines. Finally, a further molecular docking technique was employed to confirm the binding of the complexes toward the molecular target DNA.

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“…Molecular docking is considered to be a leading tool in biophysical and pharmaceutical sciences. 31,32 The aim of NPprotein docking is to complete the experimental data and estimate the predominant binding site(s) of an NP to a protein.…”

Section: Molecular Docking Studymentioning

confidence: 99%

<p>Amorphous aggregation of tau in the presence of titanium dioxide nanoparticles: biophysical, computational, and cellular studies</p>

Fardanesh¹,

Zibaie²,

Shariati³

et al. 2019

IJN

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BackgroundNanoparticles (NPs) when injected into the body can reach target tissues like nervous system and interact with tau proteins and neurons. This can trigger conformational changes of tau and may affect NP toxicity.MethodsIn this study, we used several biophysical techniques (extrinsic and intrinsic fluorescence spectroscopy, circular dichroism (CD) spectroscopy, ultraviolet (UV)-visible spectroscopy), transmission electron microscopy (TEM) investigations, molecular docking and molecular dynamics studies, and cellular assays [3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide (MTT) and flow cytometry) to reveal how structural changes of tau protein can change the cytotoxicity of titanium dioxide (TiO2) NPs against neuron-like cells (SH-SY5Y) cells.ResultsIt was shown that TiO2 NPs result in hydrophilic interactions, secondary and tertiary structural changes, and the formation of amorphous tau aggregates. Conformational changes of tau increased the induced cytotoxicity by TiO2 NPs. These data revealed that the denatured adsorbed protein on the NP surface may enhance NP cytotoxicity.ConclusionTherefore, this study provides useful insights on the NP–protein interactions and discusses how the protein corona can increase cytotoxicity to determine the efficacy of targeted delivery of nanosystems.

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Cobalt (II) complex with novel unsymmetrical tetradentate Schiff base (ON) ligand: in vitro cytotoxicity studies of complex, interaction with DNA/protein, molecular docking studies, and antibacterial activity

Cited by 41 publications

References 99 publications

Glutamine conjugated organotin(IV) Schiff base compounds: Synthesis, structure, and anticancer properties

Glutamine conjugated organotin(IV) Schiff base compounds: Synthesis, structure, and anticancer properties

Structure and cytotoxicity of zinc (II) and cobalt (II) complexes based on 1,3,5‐tris(1‐imidazolyl) benzene

<p>Amorphous aggregation of tau in the presence of titanium dioxide nanoparticles: biophysical, computational, and cellular studies</p>

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