Cobalt induced augmentation of in vitro morphogenic potential in Erythrina variegata L.: a multipurpose tree legume

Javed, Saleem; Anis, Mohammad

doi:10.1007/s11240-014-0613-2

Cited by 15 publications

(6 citation statements)

References 51 publications

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“…Adventitious rooting efficiency generally varies with the use of explant sources, choice of PGR and also with the type of species. Rooting occurs more efficiently in tender plant parts than the mature ones as the young part possess active meristemic tissues (Javed and Anis 2015). In our study, 1.0 mg l −1 NAA augmented MS was quite effective for root induction in in in vitro regenerated shoots.…”

Section: Discussionsupporting

confidence: 51%

Cadmium chloride (CdCl2) elicitation improves reserpine and ajmalicine yield in Rauvolfia serpentina as revealed by high-performance thin-layer chromatography (HPTLC)

Zafar¹,

Mujib²,

Ali³

et al. 2020

3 Biotech

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In vitro cultures play a promising role for production of pharmaceutically important plant secondary metabolites and the use of elicitation can mitigate the low productivity of active compounds. In the present study, the influence of cadmium chloride (CdCl 2 ) elicitation on alkaloid yield was investigated in Rauvolfia serpentina. This heavy metal was employed to enhance the yield of reserpine and ajmalicine in leaf derived callus, leaves, stems and roots of in vitro grown cultures. Different concentrations [0.05 mM (C1), 0.10 mM (C2), 0.15 mM (C3) and 0.20 mM (C4)] of CdCl 2 were added to the MS medium. The elicitor's influence on callus biomass, biochemical attributes and the yield of alkaloids was monitored at regular intervals. The amendment of CdCl 2 improved growth and maximum callus biomass (1.29 g fresh weight and 0.16 g dry weight) was noted at 0.15 mM (C3) after 6 days of elicitation. The addition of elicitor in medium caused cellular stress and to analyse the role of CdCl 2 in plant defence responses various antioxidant enzymes, i.e., superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) activities were measured in treated and non-treated cultures. The antioxidant enzyme activity increased linearly with elevated levels of CdCl 2 in medium; highest APX (0.88 EU min −1 mg −1 protein), SOD (5.40 EU min −1 mg −1 protein) and CAT (4.21 EU min −1 mg −1 protein) activity were observed in leaves of in vitro regenerated plants at C4. The quantitative analyses of reserpine and ajmalicine were conducted in different elicitated tissues using high-performance thin-layer chromatography (HPTLC) method. The study reveals enriched level of reserpine and ajmalicine in cultivated tissues and the enhancement was noted up to C3 (0.15 mM) elicitor level. Reserpine yield was maximum (0.191 mg g −1 DW) in roots of in vitro regenerated plants. The accumulation of ajmalicine was, however, better in leaf derived callus at C3 (0.131 mg g −1 DW). Higher elicitor dose (0.20 mM) inhibited callus biomass growth and subsequent alkaloid accumulation. The present study indicates the use of CdCl 2 as a propitious method in enhancing reserpine and ajmalicine yield in R. serpentina.

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Section: Discussionsupporting

confidence: 51%

Cadmium chloride (CdCl2) elicitation improves reserpine and ajmalicine yield in Rauvolfia serpentina as revealed by high-performance thin-layer chromatography (HPTLC)

Zafar¹,

Mujib²,

Ali³

et al. 2020

3 Biotech

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“…Cobalt is a trace element in most plant tissue culture media (39); low level of cobalt ion enhances plant growth through different mechanisms (40)(41). However, exceeding a threshold concentration of such element results in oxidative stress as indicated by appearance of oxidative stress markers (eg: MDA) (19).…”

Section: Discussionmentioning

confidence: 99%

Effect of cobalt nanoparticles and cobalt ions on alkaloids production and expression of CrMPK3 gene in Catharanthus roseus suspension cultures

Fouad

Hafez

2018

Cell Mol Biol (Noisy-le-grand)

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The capabilities of cobalt ions (Co 2+) and cobalt nanoparticles (CoNPs) in enhancing alkaloids accumulation in Catharanthus roseus suspension cultures were evaluated in relation to the expression of CrMPK3 gene. Four concentrations (5, 10, 15 and 20 mg/L) of each cobalt form were utilized in addition to control. Both forms induced oxidative stress that was more pronounced for Co 2+ treatments. A positive correlation was observed between cobalt concentration and expression of CrMPK3 gene. However, a characteristic temporal expression profile was recorded for each cobalt form. Also, positive correlations were detected between both cobalt concentration and expression of CrMPK3 gene on one hand and the activities of antioxidant enzymes (SOD and APX) and alkaloids content on the other hand. Such correlations suggest CrMPK3 gene as a common player in cobalt-induced stress signaling; regardless of cobalt form. Results revealed the higher capability of Co 2+ , compared with CoNPs, in enhancing alkaloids accumulation. However, results supported CoNPs as a novel tool in manipulating cobaltinduced alkaloids production in C. roseus.

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“…Notably, Ce6 is prepared from chlorophyll a. The extraction of chlorophyll a can be achieved from different algae or plants, including Erythrina variegate [17], Bamboo leaves [18], Erythrina oricntalis leaves, Mulberry leaves, Pennisetum purpureum, and Chlorella ellipsoidea [19] etc. In this regard, Zhang's group extracted crude chlorophyll from crude silkworm excrement and prepared Ce6 through reverse Claisen condensation, hydrolysis, and acidi cation [20].…”

Section: Introductionmentioning

confidence: 99%

Efficient Synthesis of Chlorin e6 and its Potential Photodynamic Immunotherapy in Mouse Melanoma by the Abscopal Effect

Mallik

Shrestha

Lim

et al. 2023

Preprint

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Background Photodynamic therapy (PDT) can eradicate not only cancer cells but also stimulate an anti-tumor immune response. Herein, we describe two efficient synthetic methodologies for the preparation of the second generation of photosensitizer Chlorin e6 (Ce6) from Spirulina platensis in higher yield and purity, and we address the phototoxic effect of Ce6 in vitro along with anti-tumor activity due to photodynamic therapy in vivo. Methods The use of different solvents, the duration of extraction/reaction, and the yield were analyzed and compared between the two methods during the synthesis of Ce6. The synthesized Ce6 was evaluated by TLC, HPLC, LC/MS, NMR, and studied for the anti-tumor activity of melanoma in vitro and in vivo. Melanoma B16F10 cells were seeded and phototoxicity was monitored by the MTT assay. C57BL/6 mice were transplanted with B16F10 cells for the tumor allograft model. The mice were subcutaneously inoculated on the left and right flank with 0.1 mL of B16F10 cells (1×106 cells/mL). The treated mice were intravenously injected with Ce6 of 2.5 mg/kg and then exposed to red light (660 nm) on the left flank tumors at 3 h after the injection. Results Our results revealed that the tumor was suppressed not only in the left flank but also in the right flank, where no PDT was given. The immune response was also studied by analyzing Interferon-gamma (IFN-γ), Tumor necrosis factor-alpha (TNF-α), and Interleukin-2 (IL-2) of the right flank tumors through qPCR. The upregulated expression of IFN-γ, TNF-α, and IL-2 revealed the local anti-tumor immunity due to Ce6-PDT. Conclusion The finding of this study suggest an efficient methodology of Ce6 preparation and the efficacy of Ce6-PDT as a promising anti-tumor immune response.

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Cobalt induced augmentation of in vitro morphogenic potential in Erythrina variegata L.: a multipurpose tree legume

Cited by 15 publications

References 51 publications

Cadmium chloride (CdCl2) elicitation improves reserpine and ajmalicine yield in Rauvolfia serpentina as revealed by high-performance thin-layer chromatography (HPTLC)

Cadmium chloride (CdCl2) elicitation improves reserpine and ajmalicine yield in Rauvolfia serpentina as revealed by high-performance thin-layer chromatography (HPTLC)

Effect of cobalt nanoparticles and cobalt ions on alkaloids production and expression of CrMPK3 gene in Catharanthus roseus suspension cultures

Efficient Synthesis of Chlorin e6 and its Potential Photodynamic Immunotherapy in Mouse Melanoma by the Abscopal Effect

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