Cobalt loaded electrospun poly(ε-caprolactone) grafts promote antibacterial activity and vascular regeneration in a diabetic rat model

Huang, Ziqi; Zhang, Kun; Liu, Ruihua; Li, Yang; Rafique, Muhammad; Midgley, Adam C.; Wan, Ye; Yan, Hongyuan; Si, Jianghua; Wang, T.; Chen, Cuihong; Wang, Ping; Shafiq, Muhammad; Li, Jia; Zhao, Lili; Wang, Kai

doi:10.1016/j.biomaterials.2022.121901

Cited by 45 publications

(19 citation statements)

References 82 publications

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“…Vessel volume was calculated from the acquired images. Additionally, samples were stained with immunofluorescence CD31 to assess the pro‐angiogenic ability of SHA@CM hydrogels, [ 21,22 ] and the obtained images were subjected to statistical analyses using ImageJ software.…”

Section: Methodsmentioning

confidence: 99%

A Dual Reversible Cross‐Linked Hydrogel with Enhanced Mechanical Property and Capable of Proangiogenic and Osteogenic Activities for Bone Defect Repair

Chen,

He,

Gao

et al. 2023

Macromolecular Bioscience

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The clinical treatment of bone defects presents ongoing challenges. One promising approach is bone tissue engineering (BTE), wherein hydrogels have garnered significant attention. However, the application of hydrogels in BTE is severely limited due to their poor mechanical properties, as well as their inferior proangiogenic and osteogenic activities. To address these limitations, our study aimed to develop a novel hydrogel, termed dual cross‐linked alendronate (ALN)‐Ca2+/Mg2+‐doped sulfated hyaluronic acid (SHA@CM) hydrogel, using a one‐step mixing injection molding in situ method known as the “three‐in‐one” approach. This approach enabled the simultaneous formation of Schiff‐Base crosslinking and electric attraction‐based crosslinking within the hydrogel. The Schiff‐Base crosslinking contributed to the majority of the hydrogel's mechanical strength, while the electric attraction‐based crosslinking served as a release reservoir for Ca2+/Mg2+ and ALN, promoting enhanced osteogenic activities and providing additional mechanical reinforcement to the hydrogel. Our experimental data demonstrated several favorable properties of the SHA@CM hydrogel, including satisfactory injectability, rapid gelation, self‐healing capacity, and excellent cytocompatibility. Moreover, the presence of sulfated groups and Mg2+ within the SHA@CM hydrogel exhibited pro‐angiogenic effects, while the controlled release of nanoparticles formed by Ca2+/Mg2+ and ALN further enhanced the osteogenesis of the hydrogel. These findings were supported by an in vivo study using a rat cranial defect model, wherein the SHA@CM hydrogel demonstrated enhanced therapeutic efficacy in bone defect repair. Overall, our results indicate that the SHA@CM hydrogel holds significant potential as a platform for the clinical translation of BTE.This article is protected by copyright. All rights reserved

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Section: Methodsmentioning

confidence: 99%

A Dual Reversible Cross‐Linked Hydrogel with Enhanced Mechanical Property and Capable of Proangiogenic and Osteogenic Activities for Bone Defect Repair

Chen,

He,

Gao

et al. 2023

Macromolecular Bioscience

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“…Similarly, release of therapeutic ions from inorganic NMs has been shown to promote tissue repair. Specifically, mesoporous SiO 2 , Mg(OH) 2 , cobalt (Co), copper (Cu) and BGs based scaffolds have been put forwarded and shown to stimulate tissue repair process as well as afford microbial protection and anti-inflammatory properties through release of different types of ions [ 70 , 120 , 122 , 123 , 124 ]. Consequently, release kinetics of therapeutic ions should be carefully considered to possibly predict the safe window for their therapeutic effects while safeguarding cells and tissues in vivo from toxicity risks; the overproduction of therapeutic ions may adversely affect cell viability and tissue repair process, which warrants further detailed studies.…”

Section: Conclusion and Future Outlookmentioning

confidence: 99%

Electrospinning Inorganic Nanomaterials to Fabricate Bionanocomposites for Soft and Hard Tissue Repair

Cui

Shen

et al. 2023

Nanomaterials

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Tissue engineering (TE) has attracted the widespread attention of the research community as a method of producing patient-specific tissue constructs for the repair and replacement of injured tissues. To date, different types of scaffold materials have been developed for various tissues and organs. The choice of scaffold material should take into consideration whether the mechanical properties, biodegradability, biocompatibility, and bioresorbability meet the physiological properties of the tissues. Owing to their broad range of physico-chemical properties, inorganic materials can induce a series of biological responses as scaffold fillers, which render them a good alternative to scaffold materials for tissue engineering (TE). While it is of worth to further explore mechanistic insight into the use of inorganic nanomaterials for tissue repair, in this review, we mainly focused on the utilization forms and strategies for fabricating electrospun membranes containing inorganic components based on electrospinning technology. A particular emphasis has been placed on the biological advantages of incorporating inorganic materials along with organic materials as scaffold constituents for tissue repair. As well as widely exploited natural and synthetic polymers, inorganic nanomaterials offer an enticing platform to further modulate the properties of composite scaffolds, which may help further broaden the application prospect of scaffolds for TE.

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“…PCL is a medical-grade material extensively employed in the field of medicine and biomedical engineering. , Its excellent biocompatibility, commendable mechanical properties, controllable biodegradability, and suturability render it a highly promising material for crafting artificial vascular grafts. − The degradation performance of polymers is one of the key factors determining their applicability in the field of biomedical applications. , The lifetime of the implantable devices was determined by the degradation behavior of the biomaterials. During the in vivo degradation of polymers, processes such as water uptake, thermolysis, oxidation, hydrolysis, and dissolution occur, resulting in alterations in material properties and prompting specific interactions with the host.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Vascular Grafts Using Poly(ε-Caprolactone) and Collagen-Encapsuled ADSCs for Interposition Implantation of Abdominal Aorta in Rhesus Monkeys

Jiang,

Zuo,

Wang

et al. 2024

ACS Biomater. Sci. Eng.

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The production of small-diameter artificial vascular grafts continues to encounter numerous challenges, with concerns regarding the degradation rate and endothelialization being particularly critical. In this study, porous PCL scaffolds were prepared, and PCL vascular grafts were fabricated by 3D bioprinting of collagen materials containing adipose-derived mesenchymal stem cells (ADSCs) on the internal wall of the porous PCL scaffold. The PCL vascular grafts were then implanted in the abdominal aorta of Rhesus monkeys for up to 640 days to analyze the degradation of the scaffolds and regeneration of the aorta. Changes in surface morphology, mechanical properties, crystallization property, and molecular weight of porous PCL revealed a similar degradation process of PCL in PBS at pH 7.4 containing Thermomyces lanuginosus lipase and in situ in the abdominal aorta of rhesus monkeys. The contrast of in vitro and in vivo degradation provided valuable reference data for predicting in vivo degradation based on in vitro enzymatic degradation of PCL for further optimization of PCL vascular graft fabrication. Histological analysis through hematoxylin and eosin (HE) staining and fluorescence immunostaining demonstrated that the PCL vascular grafts successfully induced vascular regeneration in the abdominal aorta over the 640-day period. These findings provided valuable insights into the regeneration processes of the implanted vascular grafts. Overall, this study highlights the significant potential of PCL vascular grafts for the regeneration of small-diameter blood vessels.

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Cobalt loaded electrospun poly(ε-caprolactone) grafts promote antibacterial activity and vascular regeneration in a diabetic rat model

Cited by 45 publications

References 82 publications

A Dual Reversible Cross‐Linked Hydrogel with Enhanced Mechanical Property and Capable of Proangiogenic and Osteogenic Activities for Bone Defect Repair

A Dual Reversible Cross‐Linked Hydrogel with Enhanced Mechanical Property and Capable of Proangiogenic and Osteogenic Activities for Bone Defect Repair

Electrospinning Inorganic Nanomaterials to Fabricate Bionanocomposites for Soft and Hard Tissue Repair

Fabrication of Vascular Grafts Using Poly(ε-Caprolactone) and Collagen-Encapsuled ADSCs for Interposition Implantation of Abdominal Aorta in Rhesus Monkeys

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