Cobalt oxide nanoparticles can enter inside the cells by crossing plasma membranes

Bossi, Elena; Zanella, Daniele; Gornati, Rosalba; Bernardini, Giovanni

doi:10.1038/srep22254

Cited by 40 publications

(25 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our investigations, conducted by TEM, confirm that zero-valent metal NPs are mainly internalized by endocytosis and persist in the cytoplasm inside vesicles [ 34 , 35 ]. These results are also supported by our previous paper in which we have proved that cobalt oxide NPs, but not zero-valent cobalt NPs, can cross the plasma membrane [ 36 ]. Conversely, SEM analysis proved that MPs are not internalized; consequently, any effects on hASCs have to be ascribed to the release of ions in the culture medium, or to the reduced oxygen and nutrients exchange efficiency, due to the presence of MP agglomerate around the cells.…”

Section: Discussionsupporting

confidence: 88%

Effects of Metal Micro and Nano-Particles on hASCs: An In Vitro Model

et al. 2017

Self Cite

View full text Add to dashboard Cite

As the knowledge about the interferences of nanomaterials on human staminal cells are scarce and contradictory, we undertook a comparative multidisciplinary study based on the size effect of zero-valent iron, cobalt, and nickel microparticles (MPs) and nanoparticles (NPs) using human adipose stem cells (hASCs) as a model, and evaluating cytotoxicity, morphology, cellular uptake, and gene expression. Our results suggested that the medium did not influence the cell sensitivity but, surprisingly, the iron microparticles (FeMPs) resulted in being toxic. These data were supported by modifications in mRNA expression of some genes implicated in the inflammatory response. Microscopic analysis confirmed that NPs, mainly internalized by endocytosis, persist in the vesicles without any apparent cell damage. Conversely, MPs are not internalized, and the effects on hASCs have to be ascribed to the release of ions in the culture medium, or to the reduced oxygen and nutrient exchange efficiency due to the presence of MP agglomerating around the cells. Notwithstanding the results depicting a heterogeneous scene that does not allow drawing a general conclusion, this work reiterates the importance of comparative investigations on MPs, NPs, and corresponding ions, and the need to continue the thorough verification of NP and MP innocuousness to ensure unaffected stem cell physiology and differentiation.

show abstract

Section: Discussionsupporting

confidence: 88%

Effects of Metal Micro and Nano-Particles on hASCs: An In Vitro Model

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…We found that nanoparticles invaded and embedded into the cell membrane. The process of iron oxide nanoparticles crossing these two lipid bilayers may be similar to that of nanoparticles crossing plasma membranes, as reported by some researchers [ 42 , 43 ]. Furthermore, cytoplasmic nanoparticles induced vacuole formation around the nanoparticles, leading to damage of the cell membrane structure and cytoplasm.…”

Section: Discussionsupporting

confidence: 66%

The Detailed Bactericidal Process of Ferric Oxide Nanoparticles on E. coli

Li¹,

Yang²,

Wang³

et al. 2018

Molecules

View full text Add to dashboard Cite

While nanoparticles exert bactericidal effects through the generation of reactive oxygen species (ROS), the processes of the internalization of and the direct physical damage caused by iron oxide nanoparticles are not completely clear. We hypothesize that direct physical or mechanical damage of the cell membrane and cytoplasmic integrity by nanoparticles is another major cause of bacterial death besides ROS. The aim of this study is to investigate the process of the internalization of iron oxide nanoparticles, and to evaluate the effect of direct physical or mechanical damage on bacterial cell growth and death. The results demonstrate that iron oxide nanoparticles not only inhibited E. coli cell growth, but also caused bacterial cell death. Iron oxide nanoparticles produced significantly elevated ROS levels in bacteria. Transmission electronic microscopy demonstrated that iron oxide nanoparticles were internalized into and condensed the cytoplasm. Strikingly, we observed that the internalized nanoparticles caused intracellular vacuole formation, instead of simply adsorbing thereon; and formed clusters on the bacterial surface and tore up the outer cell membrane to release cytoplasm. This is the first time that the exact process of the internalization of iron oxide nanoparticles has been observed. We speculate that the intracellular vacuole formation and direct physical or mechanical damage caused by the iron oxide nanoparticles caused the bactericidal effect, along with the effects of ROS.

show abstract

“…The LDH-lactate-NS electrostatically binding negative charged fluorescent dyes or ssDNA to form neutral nano-aggregates were internalized into cytosol via non-endocytic pathway without uptake inhibition of low temperature and inhibitor treatment. Bossi et al found that cobalt oxide NPs, but neither cobalt nor cobalt oxide NPs surrounded by protein corona, can enter inside the cells by penetrating plasma membranes (Bossi et al, 2016). Mature Xenopus oocytes were filled with Calcein, whose fluorescence was strongly quenched by divalent metal ions, and exposed to different NPs to quantify quenching, indicating the increase of Co 2þ concentration released from cobalt NPs located in the cytosol.…”

Section: Nanoparticle Mediated Non-endocytic Uptakementioning

confidence: 99%

Transporting carriers for intracellular targeting delivery via non-endocytic uptake pathways

Zhang

Zhu

et al. 2017

Drug Delivery

View full text Add to dashboard Cite

To develop novel therapies for clinical treatments, it increasingly depends on sophisticated delivery systems that facilitate the drugs entry into targeting cells. Profound understanding of cellular uptake routes for transporting carriers promotes the optimization of performance in drug delivery systems. Although endocytic pathway is the most important part of cellular uptake routes for many delivery systems, it suffers the trouble of enzymatic degradation of transporting carriers trapped in endosomes/lysosomes. Therefore, it is desirable to develop alternative transporting methods for delivery systems via non-endocytic pathways to achieve more effective intracellular delivery. In this review, we summarize the literature exploring transporting carriers that mediate intracellular delivery via non-endocytic pathways to present the current research status in this field. Cell-penetrating peptides, pH (low) insertion peptides, and nanoparticles are categorized to exhibit their ability to directly transport various cargos into cytoplasm via non-endocytic uptake in different cell lines. It is hoped that this review can spur the interesting on development of drug delivery systems via non-endocytic uptake pathway.

show abstract

Cobalt oxide nanoparticles can enter inside the cells by crossing plasma membranes

Cited by 40 publications

References 50 publications

Effects of Metal Micro and Nano-Particles on hASCs: An In Vitro Model

Effects of Metal Micro and Nano-Particles on hASCs: An In Vitro Model

The Detailed Bactericidal Process of Ferric Oxide Nanoparticles on E. coli

Transporting carriers for intracellular targeting delivery via non-endocytic uptake pathways

Contact Info

Product

Resources

About