Cobalt Protoporphyrin Blocks EqHV-8 Infection via IFN-α/β Production

Li, Liangliang; Hu, Xinyao; Li, Shuwen; Li, Ying; Zhao, Shengmiao; Shen, Fengzhen; Wang, Changfa; Li, Yubao; Wang, Tongtong

doi:10.3390/ani13172690

Cited by 3 publications

(6 citation statements)

References 38 publications

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“…The EqHV-8 isolates were used for this study as follows: SDLC66 (GenBank: MW816102.1), SD2020113 (GenBank: MW822570.1), donkey/Shandong/10/2021 (GenBank: OL856098.1). These EqHV-8 strains were proliferated in MDBK cells, and titrated by a plaque formation assay as previous reported ( Li et al., 2023 ). The anti-EqHV-8-positive serum and mouse anti-gD polyclonal antibody were prepared in our lab.…”

Section: Methodsmentioning

confidence: 99%

“…All primers are listed in Table 1 . The absolute quantification (qPCR) was performed to detect the copy number of EqHV-8 genome DNA as described previously ( Li et al., 2023 ). The recombinant plasmid of pMD18-T- ORF72 served as the template to calculate the EqHV-8 genome DNA copies.…”

Section: Methodsmentioning

confidence: 99%

“…The viral DNA of serum was extracted by viral DNA extraction kit (Omega Bio-Tek, USA) according to the manufacturer’s protocol. The EqHV-8 genome copies in the serum were calculated by absolute qPCR based on part ORF72 gene as previously described ( Li et al., 2023 ).…”

Section: Methodsmentioning

confidence: 99%

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Rutin prevents EqHV-8 induced infection and oxidative stress via Nrf2/HO-1 signaling pathway

Chen,

Li,

et al. 2024

Front. Cell. Infect. Microbiol.

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IntroductionThe Nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) signaling pathway has been extensively studied for its role in regulating antioxidant and antiviral responses. The Equid herpesvirus type 8 (EqHV-8) poses a significant threat to the equine industry, primarily manifesting as respiratory disease, abortions, and neurological disorders in horses and donkeys. Oxidative stress is considered a key factor associated with pathogenesis of EqHV-8 infection. Unfortunately, there is currently a dearth of therapeutic interventions available for the effective control of EqHV-8. Rutin has been well documented for its antioxidant and antiviral potential. In current study we focused on the evaluation of Rutin as a potential therapeutic agent against EqHV-8 infection.MethodsFor this purpose, we encompassed both in-vitro and in-vivo investigations to assess the effectiveness of Rutin in combatting EqHV-8 infection.Results and DiscussionThe results obtained from in vitro experiments demonstrated that Rutin exerted a pronounced inhibitory effect on EqHV-8 at multiple stages of the viral life cycle. Through meticulous experimentation, we elucidated that Rutin’s antiviral action against EqHV-8 is intricately linked to the Nrf2/HO-1 signaling pathway-mediated antioxidant response. Activation of this pathway by Rutin was found to significantly impede EqHV-8 replication, thereby diminishing the viral load. This mechanistic insight not only enhances our understanding of the antiviral potential of Rutin but also highlights the significance of antioxidant stress responses in combating EqHV-8 infection. To complement our in vitro findings, we conducted in vivo studies employing a mouse model. These experiments revealed that Rutin administration resulted in a substantial reduction in EqHV-8 infection within the lungs of the mice, underscoring the compound’s therapeutic promise in vivo.ConclusionIn summation, our finding showed that Rutin holds promise as a novel and effective therapeutic agent for the prevention and control of EqHV-8 infections.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Rutin prevents EqHV-8 induced infection and oxidative stress via Nrf2/HO-1 signaling pathway

Chen,

Li,

et al. 2024

Front. Cell. Infect. Microbiol.

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“…The DNA supernatant virus from different samples was extracted using the DNA Viral Genome Extraction Kit (Solarbio, Beijing, China). Absolute quantification PCR (qPCR) assay was performed to detect the supernatant DNA copies of EqHV-8 with recombinant plasmids pMD18-T-gD and ORF72 -F and ORF72 -R primers, as previously established method ( 10 ), and calculated by normalization to the standard curve.…”

Section: Methodsmentioning

confidence: 99%

“…Additionally, cases of abortion and neurological diseases in donkeys induced by EqHV-8 infections have been documented ( 5 , 6 ), highlighting the potential threat to the donkey industry. While the molecular mechanisms underlying EqHV-8 infections have been well documented in previous studies ( 7 – 10 ), it is crucial to note the limited availability of effective drugs for combating EqHV-8 infections ( 9 , 10 ).…”

Section: Introductionmentioning

confidence: 99%

Blebbistatin as a novel antiviral agent targeting equid herpesvirus type 8

Li,

Cui,

et al. 2024

Front. Vet. Sci.

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IntroductionEquid herpesvirus type 8 (EqHV-8) poses a significant threat to equine health, leading to miscarriages and respiratory diseases in horses and donkeys, and results in substantial economic losses in the donkey industry. Currently, there are no effective drugs or vaccines available for EqHV-8 infection control.MethodsIn this study, we investigated the in vitro and in vivo antiviral efficacy of Blebbistatin, a myosin II ATPase inhibitor, against EqHV-8.ResultsOur results demonstrated that Blebbistatin significantly inhibited EqHV-8 infection in Rabbit kidney (RK-13) and Madin-Darby Bovine Kidney (MDBK) cells in a concentration-dependent manner. Notably, Blebbistatin was found to disrupt EqHV-8 infection at the entry stage by modulating myosin II ATPase activity. Moreover, in vivo experiments revealed that Blebbistatin effectively reduced EqHV-8 replication and mitigated lung pathology in a mouse model.ConclusionCollectively, these findings suggest that Blebbistatin holds considerable potential as an antiviral agent for the control of EqHV-8 infection, presenting a novel approach to addressing this veterinary challenge.

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Protective Role of Cepharanthine Against Equid Herpesvirus Type 8 Through AMPK and Nrf2/HO-1 Pathway Activation

Li,

Sun

et al. 2024

Viruses

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Equid herpesvirus type 8 (EqHV-8) is known to cause respiratory disease and miscarriage in horses and donkeys, which is a major problem for the equine farming industry. However, there are currently limited vaccines or drugs available to effectively treat EqHV-8 infection. Therefore, it is crucial to develop new antiviral approaches to prevent potential pandemics caused by EqHV-8. This study evaluates the antiviral and antioxidant effects of cepharanthine against EqHV-8 by employing both in vitro assays and in vivo mouse models to assess its therapeutic efficacy. To assess the effectiveness of cepharanthine against EqHV-8, we conducted experiments using NBL-6 and RK-13 cells. Additionally, we developed a mouse model to validate cepharanthine’s effectiveness against EqHV-8. In our in vitro experiments, we assessed the cepharanthine’s ability to inhibit infection caused by EqHV-8 in NBL-6 and RK-13 cells. Our results demonstrated that cepharanthine has a dose-dependent inhibitory effect, indicating that it possesses anti-EqHV-8 properties at the cellular level. Moreover, we investigated the mechanism through which cepharanthine exerts its protective effects. It was observed that cepharanthine effectively reduces the oxidative stress induced by EqHV-8 by activating the AMPK and Nrf2/HO-1 signaling pathways. Furthermore, when administered to EqHV-8 infected mice, cepharanthine significantly improved lung tissue pathology and reduced oxidative stress. The findings presented herein collectively highlight cepharanthine as a promising candidate for combating EqHV-8 infections.

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Cobalt Protoporphyrin Blocks EqHV-8 Infection via IFN-α/β Production

Cited by 3 publications

References 38 publications

Rutin prevents EqHV-8 induced infection and oxidative stress via Nrf2/HO-1 signaling pathway

Rutin prevents EqHV-8 induced infection and oxidative stress via Nrf2/HO-1 signaling pathway

Blebbistatin as a novel antiviral agent targeting equid herpesvirus type 8

Protective Role of Cepharanthine Against Equid Herpesvirus Type 8 Through AMPK and Nrf2/HO-1 Pathway Activation

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