Cocaine alters catalase activity in prefrontal cortex and striatum of mice

Macêdo, Danielle Silveira; Vasconcelos, Silvânia Maria Mendes; Santos, Ronaldo Pereira; Aguiar, Lissiana Magna Vasconcelos; Lima, Vera Targino Moreira; Viana, Glauce Socorro Barros; Sousa, Francisca Cléa Florenço de

doi:10.1016/j.neulet.2005.07.024

Cited by 41 publications

(20 citation statements)

References 24 publications

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“…Figure 5a is representative of protein identification in this study. The GFAP identification was validated by sequencing two peptides, ALAAELNQLR [94][95][96][97][98][99][100][101][102][103] and LADVYQAELR, [110][111][112][113][114][115][116][117][118][119] having precursor ion m/z of 1098.6 and 1177.6, respectively (Figure 5b and c). Some of the protein spots that yielded significant DeCyder ratios were not identified with MALDI-ToF-MS, which can be attributed to insufficient amounts of protein.…”

Section: Methodological Considerationsmentioning

confidence: 99%

Cytosolic proteomic alterations in the nucleus accumbens of cocaine overdose victims

2006

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Chronic cocaine use in humans and animal models is known to lead to pronounced alterations in neuronal function in the nucleus accumbens (NAc), a brain region associated with drug reinforcement. Two-dimensional gel electrophoresis was used to compare protein alterations in the NAc between cocaine overdose (COD) victims (n = 10) and controls (n = 10). Following image normalization, spots with significantly differential image intensities (P < 0.05) were identified, excised, trypsin digested and analyzed by matrix-assisted laser desorption ionization-time of flight-time of flight. A total of 1407 spots were found to be present in a minimum of five subjects per group and the intensity of 18 spots was found to be differentially abundant between the groups, leading to positive identification of 15 proteins by peptide mass fingerprinting (PMF). Of an additional 37 protein spots that were constitutively expressed, 32 proteins were positively identified by PMF. Increased proteins in COD included b-tubulin, liprin-a3 and neuronal enolase, whereas decreased proteins included parvalbumin, ATP synthase b-chain and peroxiredoxin 2. The present data provide a preliminary protein profile of COD, suggesting the involvement of novel proteins and pathways in the expression of this complex disease. Additional studies are warranted to further characterize alterations in the differentially regulated proteins. Understanding the coordinated involvement of multiple proteins in cocaine abuse provides insight into the molecular basis of the disease and offers new targets for pharmacotherapeutic intervention for drug abuse-related disorders.

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Section: Methodological Considerationsmentioning

confidence: 99%

Cytosolic proteomic alterations in the nucleus accumbens of cocaine overdose victims

2006

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“…Cocaine exposure has been reported to increase H 2 O 2 in the prefrontal cortex and in the striatum of rats (Dietrich et al, 2005). Cocaine exposure was also shown to induce a decrease in catalase activity (Macedo et al, 2005), as well as an increase in SOD and GPx activities (Dietrich et al, 2005) in the cortex and in the striatum of animals. The levels of antioxidants such as GSH or reduced vitamin E have been also shown to be decreased upon cocaine exposure (Poon et al, 2007;Lipton et al, 2003).…”

Section: 4mentioning

confidence: 98%

Cellular and molecular mechanisms involved in the neurotoxicity of opioid and psychostimulant drugs

Cunha‐Oliveira

Rego

Oliveira

2008

Brain Research Reviews

198

136

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“…Curiously, rats exposed to cocaine selfadministration exhibited decreased MDA brain levels, but withdrawal from the drug increased MDA in the hippocampus (Pomierny-Chamiolo, Moniczewski, Wydra, Suder, & Filip, 2013). Cocaine exposure also induced a lower catalase activity in the PFC and striatum in mice (Macedo et al, 2005), but higher superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities in the same brain areas in rats (Dietrich et al, 2005). Nonenzymatic antioxidant levels such as reduced glutathione (GSH) or vitamin (vit) E, were also decreased upon cocaine exposure (Lipton et al, 2003;Poon et al, 2007).…”

Section: Cocaine and Oxidative Stressmentioning

confidence: 99%