Cocaine- and amphetamine-regulated transcript peptide- and dopamine-containing systems interact in the ventral tegmental area of the zebra finch, Taeniopygia guttata, during dynamic changes in energy status

Mitra, Saptarsi; Basu, Sumela; Singh, O. P.; Lechan, Ronald M.; Singru, Praful S.

doi:10.1007/s00429-021-02348-y

Cited by 5 publications

(18 citation statements)

References 81 publications

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Section: Subtypes Of Th-i Neurons In the Vta And Sn Of T Guttatasupporting

confidence: 81%

“…As described previously (Bottjer, 1993;Mitra et al, 2021;Nordeen et al, 2009;Person et al, 2008), TH-i neurons and fibers were observed in the VTA and SN of T. guttata (Figure 7a-c). The TH-i neurons in the VTA were smaller than those in the SN (Mitra et al, 2021) (Figure 7b,c, Table 3). The cellular (CD) and nuclear (ND) diameters of the TH-i neurons in the VTAa (CD: 10.53 ± 0.94 µm and ND: 6.52 ± 0.75 µm), VTAm (CD: 10.35 ± 1.13 µm and ND: 6.74 ± 0.94 µm), and VTAc (CD: 10.41 ± 0.34 µm and ND: 6.66 ± 1.07 µm) were comparable (p > .05), whereas those in SN (CD: 19.78 ± 1.63 µm and ND: 9.01 ± 0.69 µm; p < .001) were larger than the TH neurons in VTA.…”

Section: Subtypes Of Th-i Neurons In the Vta And Sn Of T Guttatasupporting

confidence: 81%

“…Equipped with a well-defined VTA and SN in the midbrain, their role in regulation of energy balance (Mitra et al, 2021), behavior (Alger et al, 2011;Barr & Woolley, 2018;Goodson et al, 2009), projection are comparable with the information available in the midbrain of mice where higher expressions of Calb1 and Calb2 and undetectable Pvalb mRNA were observed in the VTA and SN of mice (Chung et al, 2005;Poulin et al, 2014). The levels of Calb2, Pvalb, and Scgn mRNA were correlated with the CR-, PV-, and SCGN-ir in the midbrain of T. guttata.…”

Section: Discussionmentioning

confidence: 99%

“…The organization of VTA and SN in the midbrain of T. guttata is shown in Figures 3a-i and 4-6. As described previously (Bottjer, 1993;Mitra et al, 2021;Nordeen et al, 2009), the VTA and SN resides in the ventral midbrain of T. guttata. Further, the medially located VTA comprises small sized neurons distributed in VTAa, VTAm, and VTAc, whereas SN extends mediolaterally and contains larger neurons.…”

Section: Organization Of Vta and Sn In The Midbrain Of T Guttatamentioning

confidence: 99%

“…courtship (Goodson et al, 2009;Huang & Hessler, 2008;Sasaki et al, 2006), and regulation of energy balance (Mitra et al, 2021).…”

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confidence: 99%

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Calcium‐binding proteins typify the dopaminergic neuronal subtypes in the ventral tegmental area of zebra finch,Taeniopygia guttata

Mitra

Basu

Singh

et al. 2022

J of Comparative Neurology

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Calcium-binding proteins (CBPs) regulate neuronal function in midbrain dopamine (DA)-ergic neurons in mammals by buffering and sensing the intracellular Ca 2+ , and vesicular release. In birds, the equivalent set of neurons are important in song learning, directed singing, courtship, and energy balance, yet the status of CBPs in these neurons is unknown. Herein, for the first time, we probe the nature of CBPs, namely, Calbindin-, Calretinin-, Parvalbumin-, and Secretagogin-expressing DA neurons in the ventral tegmental area (VTA) and substantia nigra (SN) in the midbrain of zebra finch, Taeniopygia guttata. qRT-PCR analysis of ventral midbrain tissue fragment revealed higher Calbindin-and Calretinin-mRNA levels compared to Parvalbumin and Secretagogin. Application of immunofluorescence showed CBP-immunoreactive (-i) neurons in VTA (anterior [VTAa], mid [VTAm], caudal [VTAc]), SN (compacta [SNc], and reticulata [SNr]). Compared to VTAa, higher Calbindin-and Parvalbumin-immunoreactivity (-ir), and lower Calretinin-ir were observed in VTAm and VTAc. Secretagogin-ir was highly localized to VTAa. In SN, Calbindin-and Calretinin-ir were higher in SNc, SNr was Parvalbumin enriched, and Secretagogin-ir was not detected. Weak, moderate, and intense tyrosine hydroxylase (TH)-i VTA neurons were demarcated as subtypes 1, 2, and 3, respectively. While subtype 1 TH-i neurons were neither Calbindin-nor Calretinin-i, ∼80 and ∼65% subtype 2 and ∼30 and ∼45% subtype 3 TH-i neurons co-expressed Calbindin and Calretinin, respectively. All TH-i neuronal subtypes coexpressed Parvalbumin with reciprocal relationship with TH-ir. We suggest that the CBPs may determine VTA DA neuronal heterogeneity and differentially regulate their activity in T. guttata.

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Section: Subtypes Of Th-i Neurons In the Vta And Sn Of T Guttatasupporting

confidence: 81%

Section: Subtypes Of Th-i Neurons In the Vta And Sn Of T Guttatasupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Section: Organization Of Vta and Sn In The Midbrain Of T Guttatamentioning

confidence: 99%

“…courtship (Goodson et al, 2009;Huang & Hessler, 2008;Sasaki et al, 2006), and regulation of energy balance (Mitra et al, 2021).…”

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confidence: 99%

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Calcium‐binding proteins typify the dopaminergic neuronal subtypes in the ventral tegmental area of zebra finch,Taeniopygia guttata

Mitra

Basu

Singh

et al. 2022

J of Comparative Neurology

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Cocaine‐ and Amphetamine‐Regulated Transcript Peptide in the Central Nervous System of the Gecko, Hemidactylus leschenaultii: Molecular Characterization, Neuroanatomical Organization, and Regulation by Neuropeptide Y

Singh,

Basu,

Srivastava

et al. 2024

J of Comparative Neurology

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Neuropeptide cocaine‐ and amphetamine‐regulated transcript (CART) is widely expressed in the brains of teleosts, amphibians, birds, and mammals and has emerged as a conserved regulator of energy balance across these vertebrate phyla. However, as yet, there is no information on CART in the reptilian brain. We characterized the cDNA encoding CART and mapped CART‐containing elements in the brain of gecko, Hemidactylus leschenaultii (hl) using a specific anti‐CART antiserum. We report a 683‐bp hlcart transcript containing a 336‐bp open reading frame, which encodes a putative 111‐amino acid hl‐preproCART. The 89‐amino acid hl‐proCART generated from hl‐preproCART produced two putative bioactive hl‐CART‐peptides. These bioactive CART‐peptides were > 93% similar with those in rats/humans. Although reverse transcription‐polymerase chain reaction (RT‐PCR) detected hlcart‐transcript in the brain, CART‐containing neurons/fibers were widely distributed in the telencephalon, diencephalon, mesencephalon, rhombencephalon, spinal cord, and retina. The mitral cells in olfactory bulb, neurons in the paraventricular, periventricular, arcuate (Arc), Edinger–Westphal, and brainstem nuclei were intensely CART‐positive. In view of antagonistic roles of neuropeptide Y (NPY) and CART in energy balance in the framework of mammalian hypothalamus, we probed CART–NPY interaction in the hypothalamus of H. leschenaultii. Double immunofluorescence showed a dense NPY‐innervation of Arc CART neurons. Ex vivo hypothalamic slices treated with NPY/NPY‐Y1‐receptor agonist significantly reduced hlcart‐mRNA levels in the Arc‐containing tissues and CART‐ir in the dorsal‐Arc. However, CART‐ir in ventral‐Arc was unaffected. NPY via Y1‐receptors may regulate energy balance by inhibiting dArc CART neurons. This study on CART in a reptilian brain fills the current void in literature and underscores the conserved feature of the neuropeptide across the entire vertebrate phyla.

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Mate calling alters expression of neuropeptide, cocaine- and amphetamine- regulated transcript (CART) in the brain of male frog Microhyla nilphamariensis

Bhargava,

Shetye,

Shewale

et al. 2023

Neuropeptides

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Cocaine- and amphetamine-regulated transcript peptide- and dopamine-containing systems interact in the ventral tegmental area of the zebra finch, Taeniopygia guttata, during dynamic changes in energy status

Cited by 5 publications

References 81 publications

Calcium‐binding proteins typify the dopaminergic neuronal subtypes in the ventral tegmental area of zebra finch,Taeniopygia guttata

Calcium‐binding proteins typify the dopaminergic neuronal subtypes in the ventral tegmental area of zebra finch,Taeniopygia guttata

Cocaine‐ and Amphetamine‐Regulated Transcript Peptide in the Central Nervous System of the Gecko, Hemidactylus leschenaultii: Molecular Characterization, Neuroanatomical Organization, and Regulation by Neuropeptide Y

Mate calling alters expression of neuropeptide, cocaine- and amphetamine- regulated transcript (CART) in the brain of male frog Microhyla nilphamariensis

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