Cochlospermum regium (Mart. ex Schrank) Pilg.: Evaluation of chemical profile, gastroprotective activity and mechanism of action of hydroethanolic extract of its xylopodium in acute and chronic experimental models

Arunachalam, Karuppusamy; Damazo, Amílcar Sabino; Pavan, Eduarda; Oliveira, Darley Maria; Figueiredo, Fabiana de Freitas; Machado, Marco Tulio Marra; Balogun, Sikiru Olaitan; Soares, Ilsamar Mendes; Barbosa, Robson dos Santos; Alvim, Tarso da Costa; Ascêncio, Sérgio Donizeti; Martins, Domingos Tabajara de Oliveira

doi:10.1016/j.jep.2019.01.002

Cited by 37 publications

(31 citation statements)

References 42 publications

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“…It is familiar that in addition to inflammation, the excessive generation of ROS also affects oxidative stress, which leads to altering the endogenous antioxidant defense system, including SOD and GSH (Arunachalam et al, 2019). The main source of ROS in ethanol-damaged gastric tissue is infiltration of activated neutrophils, which MPO is a crucial indicator of neutrophil infiltration in ulcer-induced injuries (Arab et al, 2015;Paulrayer et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Rutaecarpine Ameliorates Ethanol-Induced Gastric Mucosal Injury in Mice by Modulating Genes Related to Inflammation, Oxidative Stress and Apoptosis

Ren

Wei

et al. 2020

Front. Pharmacol.

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Background: Rutaecarpine (RUT), a major quinazolino carboline alkaloid compound from the dry unripe fruit Tetradium ruticarpum (A. Juss.) T. G. Hartley, has various pharmacological effects. The aim of this present study was to investigate the potential gastroprotective effect of rutaecarpine on ethanol-induced acute gastric mucosal injury in mice and associated molecular mechanisms, such as activating Nrf2 and Bcl-2 via PI3K/AKT signaling pathway and inhibiting NF-κB.Methods: Gastric ulcer index and histopathology was carried out to determine the efficacy of RUT in gastric ulceration, and the content of SOD, GSH in serum and CAT, MDA, MPO, TNF-α, IL-6, IL-1β in tissue were measured by kits. Besides, in order to illustrate the potential inflammatory, oxidative, and apoptotic perturbations, the mRNA levels of NF-κB p65, PI3K, AKT, Nrf2, Nqo1, HO-1, Bcl-2 and Bax were analyzed. In addition, the protein expression of NF-κB p65 and Nrf2 in cytoplasm and nucleus, AKT, p-AKT, Bcl-2 Bax and Caspase 3 were analyzed for further verification. Finally, immunofluorescence analysis was performed to further verify nuclear translocation of NF-κB p65.Results: Current data strongly demonstrated that RUT alleviated the gross gastric damage, ulcer index and the histopathology damage caused by ethanol. RUT inhibited the expression and nuclear translocation of NF-κB p65 and the expression of its downstream signals, such as TNF-α, IL-6, IL-1β and MPO. Immunofluorescence analysis also verifies the result. In the context of oxidative stress, RUT improved the antioxidant milieu by remarkably upregulating the expression Nqo1 and HO-1 with activating Nrf2, and could remarkably upregulate antioxidant SOD, GSH, CAT and downregulate levels of MDA. Additionally, RUT activate the expression of Bcl-2 and inhibited the expression of downstream signals Bax and Caspase 3 to promote gastric cellular survival. These were confirmed by RUT activation of the PI3K/AKT pathway manifested by enhanced expression of PI3K and promotion of AKT phosphorylation.Conclusion: Taken together, these results strongly demonstrated that RUT exerted a gastroprotective effect against gastric mucosal injury induced by ethanol. The underlying mechanism might be associated with the improvement of anti-inflammatory, anti-oxidation and anti-apoptosis system.

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Section: Discussionmentioning

confidence: 99%

Rutaecarpine Ameliorates Ethanol-Induced Gastric Mucosal Injury in Mice by Modulating Genes Related to Inflammation, Oxidative Stress and Apoptosis

Ren

Wei

et al. 2020

Front. Pharmacol.

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“…It has been studied that NSAIDs exert anti-inflammatory and calmative effects via COX-2 suppression, while inhibition of COX-1 by NSAIDs caused gastrointestinal toxicity [ 3 ]. Based on the fact that present treatments for indomethacin-induced gastric mucosal lesions are accompanied by adverse effects, including nephrotoxicity, hepatotoxicity, gynecomastia, and impotence [ 25 ], which limit their clinical application, new therapeutic strategies to prevent gastric lesions are urgently needed. Recently, there has been renewed interest in natural compounds because of fewer side effects and their broad spectrum of biological activity [ 26 – 29 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of Cuttlebone on Healing of Indomethacin‐Induced Acute Gastric Mucosal Lesions in Rats

Qiu

Yao

Fang

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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The continuing use of nonsteroidal anti-inflammatory drugs (NSAIDs) usually increases the side effects such as peptic ulcer and acute gastric lesions in the gastrointestinal tract. Cuttlebone (CB), isolated from Sepiella maindroni de Rochebrune, was reported to have antioxidant activities, but its role in the treatment of indomethacin-induced gastric lesions has not yet been confirmed. In this research, we investigate the protective effect of cuttlebone on indomethacin-related ulcers in rats and possible mechanisms. Here, gastric ulcers were induced by oral administration of indomethacin, and then the rats were treated with omeprazole (4 mg/kg) or different doses (750, 1500, and 3000 mg/kg of body weight) of cuttlebone. We evaluated lesion index, inflammation score, and a series of oxidant/antioxidant parameters. The data demonstrated that cuttlebone could protect against gastric ulcers induced by indomethacin in a dose-dependent manner (positive correlation). Also, these effects were associated with attenuating the expression of malonaldehyde (MDA) and increasing the levels of some protective ingredients like epidermal growth factor (EGF), prostaglandin E2 (PGE2), and superoxide dismutase (SOD). Thus, considering its ability to protect indomethacin-induced acute gastric mucosal lesions and the underlying mechanisms, CB might be a potential candidate for treating gastric damage caused by NSAIDs.

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“…Importantes classes farmacologimanete ativas são observadas em todos os principais órgãos de C. regium, tornando este, um importante vegetal altamente valorizado para a pesquisa e produção de biofármacos. Arunachalam et al (2019), identificaram a presença de rutina com média de 16,31 µg mg -1 , miricetina 13,44 µg mg -1 e morin com 0,62 µg mg -1 no extrato etanólico do xilopódio (raiz) de C. regium. Nos estudos de Pedroso et al (2019), os pesquisadores obtiveram importantes resultados de compostos químicos no extrato hidrometanólico das raizes de C. regium.…”

Section: Química E Classes De Compostos Farmacologicamente Ativosunclassified

“…Nos estudos de Arunachalam et al (2019), os pesquisadores avaliaram a ação de gastroproteção do extrato etanólico do xilopódio de C. regium, onde observaram excepcional ação contra a bactéria Helicobacter pylori. Os pesquisadores ainda avaliaram em ratos tratados com concentrações do extrato hidroetanólico, onde apresentaram redução glicêmica em jejum.…”

Section: Atividades Biológicasunclassified

“…É uma espécie terrícola, arbustiva, que apresenta ocorrência frequente em áreas de Cerrado da região Centro-Oeste do Brasil (Camillo et al, 2009). O C. regium apresenta grande e profundo rizoma axial carnoso, numerosas folhas por seguimento do tipo simples e palmatilobadas, apresentando aroma característico quando esmagadas; são plantas lenhosas, com floração anual apresentando flores vistosas de fácil identificação, de coloração amarela, levemente aromáticas do tipo terminal, paniculada e hermafroditas, florescendo entre os meses de maio-agosto; os frutos são do tipo secos, capsulares deiscentes, loculicida, oblongos, também com aroma característico, e numeroras sementes reniformes, de testa lisa, crustácea, de coloração castanho-escuras, densamente tomentosas, pilosas, sedosas por fruto Castro, 2019;Arunachalam et al, 2019;Joly, 2002;Kirisawa, 1981).…”

Section: Introductionunclassified

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Cochlospermum regium: conservação e atividade química e biológica

Filho

2020

J. Biotechnol. Biodivers.

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O Cochlospermum regium é uma importante espécie vegetal tanto para o meio florístico, quanto para a fitoterapia. A espécie apresenta desenvolvimento por sementes sendo necessário protocolos para quebra de dormência e altas taxas de porcentagem de germinação, visto que, é uma planta explorada diariamente no campo, onde a população e raizeiros buscam o órgão terrestre (rizoma ou xilopódio) para o preparo de garrafadas e pós para consumo, utilizado para aliviar e tratar numerosas formas infecciosas. Desta forma, o trabalho visou levantar dados bibliográficos de estudos dentre eles, artigos, livros, dissertações de mestrados sobre a conservação da espécie observando dados sobre a quebra de dormência e germinação, e sobre as atividades química e biológica exercida pelas inúmeras formas em que os órgãos vegetais de C. regium são utilizadas. Através das revisões foi possível verificar inúmeros protocolos de germinação e vários trabalhos onde visaram identificar as classes de compostos e suas atividades biológicas. Nesta revisão é possível observar que o C. regium é exaustivamente estudado possibilitando que novos fármacos e meios de conservação de alimentos através das ações antioxidantes e microbianas possibilitem o uso desta espécie vegetal. Além disso, os protocolos apresentados neste estudo, apresentam altas taxas de germinação, podendo auxiliar futuros projetos de reflorestamento e inclusão de C. regium em outros ambientes ou mesmo em outros biomas perpetuando a espécie.

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Cochlospermum regium (Mart. ex Schrank) Pilg.: Evaluation of chemical profile, gastroprotective activity and mechanism of action of hydroethanolic extract of its xylopodium in acute and chronic experimental models

Cited by 37 publications

References 42 publications

Rutaecarpine Ameliorates Ethanol-Induced Gastric Mucosal Injury in Mice by Modulating Genes Related to Inflammation, Oxidative Stress and Apoptosis

Rutaecarpine Ameliorates Ethanol-Induced Gastric Mucosal Injury in Mice by Modulating Genes Related to Inflammation, Oxidative Stress and Apoptosis

Effect of Cuttlebone on Healing of Indomethacin‐Induced Acute Gastric Mucosal Lesions in Rats

Cochlospermum regium: conservação e atividade química e biológica

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