Cocirculation of Rio Negro Virus (RNV) and Pixuna Virus (PIXV) in Tucumán province, Argentina

Pisano, María Belén; Dantur, M. J.; Ré, Viviana Elizabeth; Díaz, Luis Adrián; Farías, Adrián; Seco, María Paz Sánchez; Tenorio, Antonio; Almirón, Walter Ricardo; Contigiani, Marta Silvia

doi:10.1111/j.1365-3156.2010.02541.x

Cited by 18 publications

(13 citation statements)

References 11 publications

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“…Strains of Río Negro virus of the VEEV complex were sequenced from a pool of Cx. mollis from S.M de Tucumán (Pisano et al 2010), and Cx. interfor is considered to be a secondary vector of the Saint Louis Equine Encephalitis virus (Spinsanti et al 2009) in Córdoba.…”

Section: Introductionmentioning

confidence: 99%

Discrimination of four Culex (Culex) species from the Neotropics based on geometric morphometrics

2015

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Achieving correct identification of Culex species is difficult because many anatomical characters of larvae and females are polymorphic or overlap among distinct species. The overlapping is known to occur between Culex bidens, Cx. interfor, Cx. mollis and Cx. tatoi, from the subgenus Culex. The first three were incriminated in viruses' transmission in Argentina. The purpose was to distinguish between specimens of four species using geometric morphometric procedures. From field and entomological collections, 10 type I and 10 type II landmarks on the wings of females and the dorsomentum of larvae, respectively, were defined. The free morphometric software modules by J.P. Dujardin were used. Landmark coordinates were submitted to Procrustes and TPS analyses to generate size (centroid size, CS) and shape (partial warps, PW) variables. Size analysis was performed by nonparametric comparisons of CS measurements based on permutations, and shape, by submission of PW to discriminant analysis. Re-identification and identification of external specimens were also realized. Wing and Dm shapes gave similar results. The individuals arranged into three groups coinciding with Cx. bidens ? Cx. interfor, Cx. mollis and Cx. tatoi. The latter two had a very different wing and Dm shapes. The accurate re-identification was greater than or equal to 77 % in all cases, and the identification of external specimens was achieved for all the species and both structures. Larval characters are more informative than female features. Geometric morphometrics, as a complementary tool, will facilitate identification in junction with others, such as DNA sequence analysis.

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Section: Introductionmentioning

confidence: 99%

Discrimination of four Culex (Culex) species from the Neotropics based on geometric morphometrics

2015

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“…Mosquitoes with PIXV bite and infect birds, rodents, domestic animals and humans. Although PIXV was first isolated from Anopheles ( Stethomyia ) nimbus mosquitoes in Brazil, in 1961 4 and has been detected later in Argentina 5 , little is known about the mechanisms concerning the cell biology of viral infection.…”

Section: Introductionmentioning

confidence: 99%

Pixuna virus modifies host cell cytoskeleton to secure infection

Gil

Albrieu-Llinás

Mlewski

et al. 2017

Sci Rep

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Pixuna virus (PIXV) is an enzootic member of the Venezuelan Equine Encephalitis Virus complex and belongs to the New World cluster of alphaviruses. Herein we explore the role of the cellular cytoskeleton during PIXV replication. We first identified that PIXV undergoes an eclipse phase consisting of 4 h followed by 20 h of an exponential phase in Vero cells. The infected cells showed morphological changes due to structural modifications in actin microfilaments (MFs) and microtubules (MTs). Cytoskeleton-binding agents, that alter the architecture and dynamics of MFs and MTs, were used to study the role of cytoskeleton on PIXV replication. The virus production was significantly affected (p < 0.05) after treatment with paclitaxel or nocodazole due to changes in the MTs network. Interestingly, disassembly of MFs with cytochalasin D, at early stage of PIXV replication cycle, significantly increased the virus yields in the extracellular medium (p < 0.005). Furthermore, the stabilization of actin network with jasplakinolide had no effect on virus yields. Our results demonstrate that PIXV relies not only on intact MTs for the efficient production of virus, but also on a dynamic actin network during the early steps of viral replication.

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“…Additionally, serological studies have demonstrated the presence of antibodies against subtypes VI and I (strain TC83) in inhabitants of General Belgrano Island (Cámara et al 2003). Recent investigations have detected RNV in mosquitoes from Chaco (Pisano et al 2010b) and, for the first time, in mosquitoes from the province of Tucumán, demonstrating a wider distribution in the northern areas of Argentina (Pisano et al 2010a) than previously suspected. In these provinces, Pixuna virus (PIXV), another enzootic member of the VEE complex (subtype IV), has also been detected, sometimes circulating within the same region and at the same time as RNV (Pisano et al 2010a).…”

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confidence: 72%

First detection of Rio Negro virus (Venezuelan equine encephalitis complex subtype VI) in Córdoba, Argentina

Pisano

Spinsanti

Díaz

et al. 2012

Mem. Inst. Oswaldo Cruz

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Cocirculation of Rio Negro Virus (RNV) and Pixuna Virus (PIXV) in Tucumán province, Argentina

Cited by 18 publications

References 11 publications

Discrimination of four Culex (Culex) species from the Neotropics based on geometric morphometrics

Discrimination of four Culex (Culex) species from the Neotropics based on geometric morphometrics

Pixuna virus modifies host cell cytoskeleton to secure infection

First detection of Rio Negro virus (Venezuelan equine encephalitis complex subtype VI) in Córdoba, Argentina

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