Cocoa bean (Theobroma cacao L.) phenolic extracts as PTP1B inhibitors, hepatic HepG2 and pancreatic β-TC3 cell cytoprotective agents and their influence on oxidative stress in rats

Żyżelewicz, Dorota; Zakłos‐Szyda, Małgorzata; Juśkiewicz, Jerzy; Bojczuk, Małgorzata; Oracz, Joanna; Budryn, Grażyna; Miśkiewicz, Karolina; Krysiak, Wiesława; Zduńczyk, Zenon; Jurgoński, Adam

doi:10.1016/j.foodres.2016.01.009

Cited by 30 publications

(30 citation statements)

References 60 publications

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“…Each experiment was performed at least in triplicate. Statistical values comparing sample groups were determined using the Student's t test and one-way analysis of variance ANNOVA using Statistica 10.0 software at the significance level of p ≤ 0.05 [22].…”

Section: Discussionmentioning

confidence: 99%

“…Intracellular ROS generation was evaluated and expressed as fluorescence % of control (cells without coffee extracts). As a positive oxidative stress inducer, 500 μM t-BOOH was used [22]. The assay was performed in the presence of serum-free medium with phenol red.…”

Section: Detection Of Intracellular Reactive Oxygen Species (Ros)mentioning

confidence: 99%

“…After treatment, the cells were washed with cold PBS, fixed in 5% paraformaldehyde for 30 min and stained with Nile red solution (1 μg/mL) for 40 min at 37 °C and 5% CO 2 ; the lipid-bound Nile red fluorescent signal F485/530 was measured. Lipid accumulation was evaluated and expressed as fluorescence % of control (cells without coffee extract treatment) [22].…”

Section: Lipid Accumulation Assaymentioning

confidence: 99%

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Green and roasted coffee extracts as antioxidants in βTC3 cells with induced oxidative stress and lipid accumulation inhibitors in 3T3L1 cells, and their bioactivity in rats fed high fat diet

Budryn

Zakłos‐Szyda

Zaczyńska

et al. 2017

Eur Food Res Technol

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Section: Discussionmentioning

confidence: 99%

Section: Detection Of Intracellular Reactive Oxygen Species (Ros)mentioning

confidence: 99%

Section: Lipid Accumulation Assaymentioning

confidence: 99%

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Green and roasted coffee extracts as antioxidants in βTC3 cells with induced oxidative stress and lipid accumulation inhibitors in 3T3L1 cells, and their bioactivity in rats fed high fat diet

Budryn

Zakłos‐Szyda

Zaczyńska

et al. 2017

Eur Food Res Technol

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“…The antioxidant activity (AA) of the tested samples was calculated according to Zyzelewicz et al . () as AA (%) = (Control absorbance − Sample absorbance)/Control absorbance × 100.…”

Section: Methodsmentioning

confidence: 99%

“…Different tea extracts were added to ABTS solution, and after 30 min of incubation, absorbance of all samples was taken at 745 nm. The antioxidant activity (AA) of the tested samples was calculated according to Zyzelewicz et al (2016) as AA (%) = (Control absorbance À Sample absorbance)/Control absorbance 9 100.…”

Section: Abts Scavenging Activitymentioning

confidence: 99%

Effect of consecutive steeping on antioxidant potential of green, oolong and black tea

Islam

Farooq

Sehgal

2017

Int J of Food Sci Tech

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SummaryAfter water, tea is considered to be one of the most consumed drinks worldwide. To get the maximum benefit from the whole-leaf teas, the manufacturers provide instruction that these teas can be steeped 2-3 times over 6-8 h. In this study, the effect of consecutive steeping on radical-scavenging ability of whole leaf green tea (unfermented), oolong tea (partially fermented) and black tea (fully fermented) was determined. Green tea showed the highest level of antioxidant activity, total phenolic and flavonoid content followed by oolong tea and black tea irrespective of the number of steeping done. The result also demonstrated that re-steeping reduced the antioxidant potential of teas. Moreover, it was revealed that green tea is the best candidate for re-steeping followed by oolong and then black tea.

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Centrifugal partition chromatography - A review of recent applications and some classic references

Bojczuk

Żyżelewicz

Hodurek³

2017

J. Sep. Sci.

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Countercurrent chromatography, based on liquid-liquid partitioning, has many technological variants. One of them is centrifugal partition chromatography, introduced by Wataru Murayama and Kanichi Nunogaki in 1982. This technique, like other countercurrent chromatography techniques, is based on the phenomenon of liquid-liquid partitioning between two immiscible liquid phases that stay at equilibrium. But the significant difference between this technique and others is the retention mechanism of stationary phase. In the case of centrifugal partition chromatography, this mechanism is based on hydrostatic force, formed by the centrifugal field in the rotor in one-axis centrifuge. Sometimes that allows more control of stationary phase, for example, when aqueous two-phase and other difficult solvent systems are used. However, the efficiency of the separation in centrifugal partition chromatography is also affected by a variety of parameters dependent on the sample properties in the solvent system, physical properties of the solvent system, parameters of the instrument, and the method. This article includes also recent ideas for improvements to the technique and broadening its application (e.g., (multiple) dual-mode or elution-extrusion procedure, pH-zone-refining centrifugal partition chromatography, ion-exchange centrifugal partition chromatography, online and offline coupling of centrifugal partition chromatography).

show abstract

Cocoa bean (Theobroma cacao L.) phenolic extracts as PTP1B inhibitors, hepatic HepG2 and pancreatic β-TC3 cell cytoprotective agents and their influence on oxidative stress in rats

Cited by 30 publications

References 60 publications

Green and roasted coffee extracts as antioxidants in βTC3 cells with induced oxidative stress and lipid accumulation inhibitors in 3T3L1 cells, and their bioactivity in rats fed high fat diet

Green and roasted coffee extracts as antioxidants in βTC3 cells with induced oxidative stress and lipid accumulation inhibitors in 3T3L1 cells, and their bioactivity in rats fed high fat diet

Effect of consecutive steeping on antioxidant potential of green, oolong and black tea

Centrifugal partition chromatography - A review of recent applications and some classic references

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