Cocoa pod husk valorization: alkaline-enzymatic pre-treatment for propionic acid production

Vásquez, Zulma Sarmiento; Vandenberghe, Luciana Porto de Souza; Rodrigues, Cristine; Tanobe, Valcineide Oliveira de Andrade; Marín, Oranys; Pereira, Gilberto Vinícius de Melo; Rogez, Hervé; Goés-Neto, Aristóteles; Soccol, Carlos Ricardo

doi:10.1007/s10570-021-03770-5

Cited by 19 publications

(10 citation statements)

References 65 publications

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“…In turn, the Propionibacterium acidipropionici ATCC 4875 strain utilized 92% of sugars (glucose, fructose) in the medium containing the hydrolysate of Jerusalem artichoke (Liang et al 2012 ). Sarmiento-Vásquez et al ( 2021 ) cultivated Propionibacterium jensenii DSM 20274 in a medium containing used cocoa pod husk hydrolysate and pure glycerol. During 156 h of fermentation, the tested strain consumed 89% of glucose and 39% of glycerol.…”

Section: Discussionmentioning

confidence: 99%

Use of apple pomace, glycerine, and potato wastewater for the production of propionic acid and vitamin B12

Piwowarek

Lipińska

Hać‐Szymańczuk

et al. 2022

Appl Microbiol Biotechnol

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Propionic acid bacteria (PAB) are a source of valuable metabolites, including propionic acid and vitamin B12. Propionic acid, a food preservative, is synthesized from petroleum refining by-products, giving rise to ecological concerns. Due to changing food trends, the demand for vitamin B12 has been expected to increase in the future. Therefore, it is necessary to look for new, alternative methods of obtaining these compounds. This study was conducted with an aim of optimizing the production of PAB metabolites using only residues (apple pomace, waste glycerine, and potato wastewater), without any enzymatic or chemical pretreatment and enrichment. Media consisting of one, two, or three industrial side-streams were used for the production of PAB metabolites. The highest production of propionic acid was observed in the medium containing all three residues (8.15 g/L, yield: 0.48 g/g). In the same medium, the highest production of acetic acid was found — 2.31 g/L (0.13 g/g). The presence of waste glycerine in the media had a positive effect on the efficiency of propionic acid production and P/A ratio. The concentration of vitamin B12 obtained in the wet biomass of Propionibacterium freudenreichii DSM 20271 ranged from 90 to 290 µg/100 g. The highest production of cobalamin was achieved in potato wastewater and apple pomace, which may be a source of the precursors of vitamin B12 — cobalt and riboflavin. The results obtained show both propionic acid and vitamin B12 can be produced in a more sustainable manner through the fermentation of residues which are often not properly managed. Key points • The tested strain has been showed metabolic activity in the analyzed industrial side-streams (apple pomace, waste glycerine, potato wastewater). • All the side-streams were relevant for the production of propinic acid. • The addition of waste glycerine increases the propionic acid production efficiency and P/A ratio. • B12 was produced the most in the media containing potato wastewater and apple pomace as dominant ingredients.

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Section: Discussionmentioning

confidence: 99%

Use of apple pomace, glycerine, and potato wastewater for the production of propionic acid and vitamin B12

Piwowarek

Lipińska

Hać‐Szymańczuk

et al. 2022

Appl Microbiol Biotechnol

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“…Composites of 7.5%, 15%, and 25% v/v, CPH were prepared by mixing filtered solutions of the raw natural rubber and filler (CPH) in appropriate amounts then casting and allowing the solvent to evaporate under a hood. Information regarding physicochemical characterization of collected cocoa pod husk can be found in the literature 21–23 . The casted rubber composites were then dried at 100°C in a vacuo to constant weight before eventual characterization.…”

Section: Methodsmentioning

confidence: 99%

Preparation and characterization of non‐vulcanized natural rubber‐based cocoa pod husk composites

Edjenguele

Alegría²,

Arrese-Igor³

et al. 2021

J of Applied Polymer Sci

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Several ingredients are often introduced into rubber matrices during the production of natural rubber (NR) based articles, essentially to modify and improve upon the properties of the final products as well as to reduce their costs. In this study, composites of natural rubber and cocoa pod husk were prepared by solution casting and tested for their rheological and thermal properties in order to ascertain the suitability of the latter as a substitute for standard fillers in the NR industry. An increase in the level of substitution significantly reduced the rheological properties of the composites in the linear viscoelastic region but had no significant effects on their thermal stability, glass transition temperature and on the α‐relaxation of the NR composites except at the highest level of substitution (25%). However, α‐relaxation and rheological shift factors were always temperature‐dependent and had a direct influence on their electrical conductivity with no permanent chemical bonds formed or broken during preparation of the composites. Hence, untreated cocoa pod husk could serve as non‐reinforcing filler for raw natural rubber.

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“…In the context of PA synthesis, bacteria of the species A. acidipropionici and P. fruedenreichii are most often used, although other PAB species have also been used, for example, A. jensenii or A. thoenii (Sarmiento-Vásquez et al al. 2021 ). The best producers of PA are bacteria of the species A. acidipropionici , which results from their greater tolerance to stress related to the acidic environment, due to the higher activity of H + -ATPase compared to other PAB.…”

Section: Pa Synthesismentioning

confidence: 99%

“…An interesting lignocellulose material that is gaining more and more popularity in the world of science is cocoa pod husk (CPH) (Sarmiento-Vásquez et al 2021 ). It is estimated that around 19 million tons of cocoa biomass residue is produced annually worldwide, which is considered worthless waste, consisting mainly of cellulose, hemicellulose and lignin (Sarmiento-Vásquez et al 2021 ). Sarmiento-Vásquez et al ( 2021 ) used CPH to synthesise PA by A. jensenii DSM 20274 bacteria.…”

Section: Pa Production From Side-streamsmentioning

confidence: 99%

“…It is estimated that around 19 million tons of cocoa biomass residue is produced annually worldwide, which is considered worthless waste, consisting mainly of cellulose, hemicellulose and lignin (Sarmiento-Vásquez et al 2021 ). Sarmiento-Vásquez et al ( 2021 ) used CPH to synthesise PA by A. jensenii DSM 20274 bacteria. CPH was subjected to chemical and enzymatic hydrolysis, which produced a material containing glucose at a level of 60.5 g/L.…”

Section: Pa Production From Side-streamsmentioning

confidence: 99%

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Reprocessing of side-streams towards obtaining valuable bacterial metabolites

Piwowarek

Lipińska

Kieliszek

2023

Appl Microbiol Biotechnol

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Every year, all over the world, the industry generates huge amounts of residues. Side-streams are most often used as feed, landfilled, incinerated, or discharged into sewage. These disposal methods are far from perfect. Taking into account the composition of the side-streams, it seems that they should be used as raw materials for further processing, in accordance with the zero-waste policy and sustainable development. The article describes the latest achievements in biotechnology in the context of bacterial reprocessing of residues with the simultaneous acquisition of their metabolites. The article focuses on four metabolites — bacterial cellulose, propionic acid, vitamin B12 and PHAs. Taking into account global trends (e.g. food, packaging, medicine), it seems that in the near future there will be a sharp increase in demand for this type of compounds. In order for their production to be profitable and commercialised, cheap methods of its obtaining must be developed. The article, in addition to obtaining these bacterial metabolites from side-streams, also discusses e.g. factors affecting their production, metabolic pathways and potential and current applications. The presented chapters provide a complete overview of the current knowledge on above metabolites, which can be helpful for the academic and scientific communities and the several industries. Key points • The industry generates millions of tons of organic side-streams each year. • Generated residues burden the natural environment. • A good and cost-effective method of side-streams management seems to be biotechnology – reprocessing with the use of bacteria. • Biotechnological disposal of side-streams gives the opportunity to obtain valuable compounds in cheaper ways: BC, PA, vitmain B12, PHAs.

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Cocoa pod husk valorization: alkaline-enzymatic pre-treatment for propionic acid production

Cited by 19 publications

References 65 publications

Use of apple pomace, glycerine, and potato wastewater for the production of propionic acid and vitamin B12

Use of apple pomace, glycerine, and potato wastewater for the production of propionic acid and vitamin B12

Preparation and characterization of non‐vulcanized natural rubber‐based cocoa pod husk composites

Reprocessing of side-streams towards obtaining valuable bacterial metabolites

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