Cocoa presents cytotoxicity against melanoma cancer cell lines (A‐375 e B16‐F10) and improves chemotherapy activity by increasing oxidative stress

Abstract

Melanoma frequently presents a poor chemotherapy response. In this scenario, investigations for new therapies are essential. Thus, cocoa is highlighted in this area since it presents many biological properties. This study investigated the anticarcinogenic activity of cocoa in melanoma cell lines (A‐375 and B16‐F10). Melanoma and fibroblast (HFF‐1) cell lines were exposed to different concentrations of cocoa seeds (30 to 2000 ug/ml) at 24 and 72 h. Cocoa was also associated with paclitaxel IC50. We conducted viability, proliferation, and oxidative stress analyses. Our findings suggested that cocoa isolated, at almost all concentrations tested, was able to reduce viability and proliferation of B16‐F10 cells and proliferation of A‐375 cells via oxidative stress increasing. Also, cocoa caused no damage in fibroblast cells. Moreover, cocoa increased paclitaxel activity on A‐375 by reducing cell proliferation and increasing oxidative stress. Therefore, the results highlight cocoa as a potent selective adjuvant anticancer agent against melanoma. Practical applications In conclusion, more studies should be performed to deeply explore this remarkable action of cocoa as a an promising adjuvant to enhance chemotherapy.

Design, Synthesis, and Anti-Cancer Evaluation of Novel Water-Soluble Copper(I) Complexes Bearing Terpyridine and PTA Ligands

“…In in vitro studies, the fibroblast or lymphoblast cell line are most often used as a reference line for normal body cells, which is why we decided to use the NHDF line, like some other authors [ 46 , 47 , 48 , 49 , 50 , 51 ], in combination with the mentioned cancer lines. Other fibroblast lines can also be used as normal cell lines [ 52 , 53 , 54 ]. In addition, the reference compounds free terpyridine and PTA, as well as copper(I) precursor [Cu(MeCN) 4 ][PF 6 ], were also tested.…”

A Comprehensive Review of Various Therapeutic Strategies for the Management of Skin Cancer

“…133 The research found that cocoa can decrease the cell viability and cell growth of melanoma cell lines, i.e., B16-F10 and A-375, by inducing oxidative stress in cells. 134 7.5.7. Myricetin.…”

“…Cocoa is a traditional medicine that can be used in the treatment of malaria, worm expellers, and wound healing . The research found that cocoa can decrease the cell viability and cell growth of melanoma cell lines, i.e., B16-F10 and A-375, by inducing oxidative stress in cells …”

Mechanism of microRNA-152-3p-mediated regulation of autophagy and sensitivity in paclitaxel-resistant ovarian cancer cells

“…Cell viability was determined by MTT assay (Sigma-Aldrich; Merck KGaA). The cells were seeded into 96well plates (1x10 4 cells/ml) for 24 h. After a preliminary literature review (Loo et al 2005; (Mello et al 2022) and pre-experiment results, A2780/A2780T cells were treated with paclitaxel at a concentration gradient of 20,15,10, 5, 2.5 µg/ml. After 48 h, 50 µL MTT (Sigma-Aldrich; Merck KGaA) was added to each well and incubated for additional 4 h. Finally, dimethyl-sulfoxide (150 µl) was added to each well, and the absorbance of the cells at 570 nm was measured using a microplate reader (SpectraMax, Molecular Devices).…”