Cocoa tea (Camellia ptilophylla) water extract inhibits adipocyte differentiation in mouse 3T3-L1 preadipocytes

Li, Kai Kai; Liu, Cheuk‐Lun; Shiu, Hoi Ting; Wong, Hai Ming; Siu, Wing Sum; Zhang, Cheng; Han, Xiao; Ye, Chuang Xing; Leung, Ping Chung; Ko, Chun‐Hay

doi:10.1038/srep20172

Cited by 61 publications

(55 citation statements)

References 69 publications

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“…Obesity has an adverse effect on health, leading to increased comorbid metabolic and chronic diseases including type-2 diabetes mellitus, hypertension, coronary heart diseases, and cancer [1,2,3,4,5]. The ideal antiobesity drug should sustain weight loss through the reduction of energy intake or the increase of energy expenditure without adverse effects.…”

Section: Introductionmentioning

confidence: 99%

Tenebrio molitor Larvae Inhibit Adipogenesis through AMPK and MAPKs Signaling in 3T3-L1 Adipocytes and Obesity in High-Fat Diet-Induced Obese Mice

Seo

Goo

Chung

et al. 2017

IJMS

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Despite the increasing interest in insect-based bioactive products, the biological activities of these products are rarely studied adequately. Larvae of Tenebrio molitor, the yellow mealworm, have been eaten as a traditional food and provide many health benefits. Therefore, we hypothesized that T. molitor larvae might influence adipogenesis and obesity-related disorders. In the present study, we investigated the anti-adipogenic and antiobesity effects of T. molitor larvae in vitro and in vivo. The lipid accumulation and triglyceride content in mature adipocytes was reduced significantly (up to 90%) upon exposure to an ethanol extract of T. molitor larvae, without a reduction in cell viability. Exposure also resulted in key adipogenic and lipogenic transcription factors. Additionally, in adipogenic differentiation medium the extract induced phosphorylation of adenosine monophosphate (AMP)-activated protein kinase and mitogen-activated protein kinases. Daily oral administration of T. molitor larvae powder to obese mice fed high-fat diet attenuated body weight gain. We also found that the powder efficiently reduced hepatic steatosis as well as aspartate and alanine transaminase enzyme levels in mice fed a high-fat diet. Our results suggest that T. molitor larvae extract has an antiobesity effect when administered as a food supplement and has potential as a therapeutic agent for obesity.

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Section: Introductionmentioning

confidence: 99%

Tenebrio molitor Larvae Inhibit Adipogenesis through AMPK and MAPKs Signaling in 3T3-L1 Adipocytes and Obesity in High-Fat Diet-Induced Obese Mice

Seo

Goo

Chung

et al. 2017

IJMS

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“…However, the effects of these test samples on lipolysis and adipogenesis as well as the mechanism underlying these effects, such as the expression of key adipogenic transcription factors including peroxisome proliferator-activated receptor gamma (PPAR γ) and CCAAT/enhancer binding protein (C/EBP α) [18], need to be explored.…”

Section: Lipid Accumulation Assaymentioning

confidence: 99%

Inhibition of Lipid Accumulation in 3t3-L1 Adipocytes by Morinda Citrifolia Linn. Leaf Extracts and Commercial Herbal Formulas for Weight Control

Saraphanchotiwitthaya

Sripalakit

2016

Int J Pharm Pharm Sci

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Objective: To determine the in vitro anti-obesity effects of Morinda citrifolia leaf extract and herbal formulas used for weight loss in Thailand on lipid accumulation in 3T3-L1 adipocytes.Methods: Differentiated 3T3-L1 adipocytes were treated with 0.1, 0.5 and 1 mg/ml M. citrifolia leaf extract, three herbal formulas (JL-RU, JL-TH, CD-H) and 1, 5 and 10 µg/ml rutin, gallic acid and caffeine. Lipid accumulation determined by measuring Oil Red O staining and triglyceride content measured by a colorimetric method in adipocytes were assayed compared to the control. The effect of test samples on the viability of preadipocytes and differentiated adipocytes were investigated.Results: Differentiated adipocytes treated with 1 mg/ml M. citrifolia extract moderately inhibited fat accumulation (45.12%) and highly reduced triglyceride content (85.09%). Among the three herbal formulas, JL-TH considerably inhibited fat accumulation (109.17%, 1 mg/ml) and decreased triglyceride content (95.00%, 1 mg/ml) in adipocytes; this was higher than that for CD-H and JL-RU, respectively. The viability of preadipocytes treated with CD-H at 1 mg/ml was slightly decreased while those treated with JL-TH at 0.05-1 mg/ml showed moderately decreased viability in a dose-response manner. For differentiated adipocytes, CD-H at 0.5-1 mg/ml moderately decreased cell viability while JL-TH at 0.05-1 mg/ml caused moderate to high reduction of cell viability in a dose-response relationship. Conclusion:M. citrifolia extract and three herbal formulas had anti-obesity effects in 3T3-L1 adipocytes, as indicated by a significant reduction in lipid accumulation, triglyceride content, and cell viability. These findings suggest a potential therapeutic approach for the prevention and treatment of obesity.

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“…Herbal extracts enriched with polyphenols, alkaloids etc., possess potent anti -adipogenic and hypoglycemic activities. 32 Our study results exhibited decreased lipid content in 70% HA and alkaloidal extracts treated group, indicative of adipogenesis inhibition in 3T3L1 adipocytes, which signifies the anti -obesity effect of MK. Metabolic imbalance in T2D results in generation of reactive oxygen species (ROS) which decreases the antioxidant levels.…”

Section: Figurementioning

confidence: 81%

Hydroalcoholic and Alkaloidal Extracts of Murraya koenigii(L.) Spreng Augments Glucose Uptake Potential against Insulin Resistance Condition in L6 Myotubes and Inhibits Adipogenesis in 3T3L1 Adipocytes

Parthasarathy¹,

Murthy²,

Girija³

et al. 2018

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Background:Murraya koenigii, commonly known as "curry leaves" is native to India. The highly valued part of the plant is the leaves which possess various biological activities. Objective: The present study aimed to investigate the antidiabetic effect of Murraya koenigii (MK) leaf extracts, of two different solvent ratios. Materials and methods: 70% hydroalcoholic and alkaloidal extracts of MK leaves were prepared by cold maceration method. Preliminary phytochemical analysis was carried out for both the extracts. In vitro anti diabetic activity was screened by inhibitory action on α -amylase, α -glucosidase enzymes. Further, the 70% hydroalcoholic and alkaloidal extracts were assessed for glucose uptake potential, anti -adipogenic property, as well as inhibitory activity on diabetes associated complications. HPTLC quantification of major phytoconstituent was carried out. Results: The study showed presence of various phytoconstituents such as, polyphenols, alkaloids, tannins, reducing sugars etc. The 70% hydroalcoholic and alkaloidal extracts of MK leaves exhibited >90% inhibition against carbohydrate metabolising enzymes compared to aqueous and absolute alcohol extracts. Both the extracts showed enhanced glucose uptake in L6 myotubes attenuating the effect of Palmitate induced insulin resistance. Significant inhibition on adipogenesis was exerted by both 70% hydroalcoholic and alkaloidal extracts of MK leaves. Besides, marked inhibition of advanced glycation end products was exhibited by the extracts. HPTLC quantification analysis of the aforementioned extracts showed the presence of major phytoconstituent, Mahanine, in it. Conclusion: The results of the present study showed that MK possesses significant antidiabetic property and also exhibited considerable effect in preventing diabetes associated complications. The potent antidiabetic activity of MK could be attributed to the presence of Mahanine, the major active constituent, which is a carbazole alkaloid.

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Cocoa tea (Camellia ptilophylla) water extract inhibits adipocyte differentiation in mouse 3T3-L1 preadipocytes

Cited by 61 publications

References 69 publications

Tenebrio molitor Larvae Inhibit Adipogenesis through AMPK and MAPKs Signaling in 3T3-L1 Adipocytes and Obesity in High-Fat Diet-Induced Obese Mice

Tenebrio molitor Larvae Inhibit Adipogenesis through AMPK and MAPKs Signaling in 3T3-L1 Adipocytes and Obesity in High-Fat Diet-Induced Obese Mice

Inhibition of Lipid Accumulation in 3t3-L1 Adipocytes by Morinda Citrifolia Linn. Leaf Extracts and Commercial Herbal Formulas for Weight Control

Hydroalcoholic and Alkaloidal Extracts of Murraya koenigii(L.) Spreng Augments Glucose Uptake Potential against Insulin Resistance Condition in L6 Myotubes and Inhibits Adipogenesis in 3T3L1 Adipocytes

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