Coculture of endothelial and smooth muscle cells on a collagen membrane in the development of a small-diameter vascular graft

Wu, Hsi Chin; Wang, Tzu‐Wei; Kang, Pei; Tsuang, Yang Hwei; Sun, Jui Sheng; Lin, Feng Huei

doi:10.1016/j.biomaterials.2006.11.012

Cited by 84 publications

(58 citation statements)

References 21 publications

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“…Due to the sensitivity of PLGA to temperature, PDMS is cured in an oven at 45 °C when using it with PLGA. Compared with previous methods for the fabrication of vessel-like structures, [9,32] the advantages of our method are unique: 1) each kind of cell is precisely distributed at a microscale resolution in 3D space, and cell orientation can also be controlled inside the tubes. Doing so makes full use of the versatility and high level of control of these techniques for patterning cells in 2D, which is intrinsically more straightforward than doing so in 3D space.…”

Section: Doi: 101002/adma201104589mentioning

confidence: 97%

A Strategy for Depositing Different Types of Cells in Three Dimensions to Mimic Tubular Structures in Tissues

Yuan¹,

Jin²,

Sun³

et al. 2012

Advanced Materials

240

195

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The fabrication of tubular structures, with multiple cell types forming different layers of the tube walls, is described using a stress-induced rolling membrane (SIRM). Cell orientation inside the tubes can also be controlled by topographical contact guidance. These layered tubes precisely mimic blood vessels and many other tubular structures, suggesting that they may be of great use in tissue engineering.

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Section: Doi: 101002/adma201104589mentioning

confidence: 97%

A Strategy for Depositing Different Types of Cells in Three Dimensions to Mimic Tubular Structures in Tissues

Yuan¹,

Jin²,

Sun³

et al. 2012

Advanced Materials

240

195

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“…Replacement vessels may be comprised of autologous tissue or synthetic biomaterials [12]. Autologous tissue refers to tissue harvested from and implanted into the same patient.…”

Section: Current Medical Treatmentsmentioning

confidence: 99%

“…Autologous tissue refers to tissue harvested from and implanted into the same patient. The saphenous vein in the leg and the internal mammary artery in the chest are most often used as autologous vessel sources for transplantation [12]. Typical synthetic graft materials may include expanded poly[tetrafloroethlyene] (ePTFE) or poly [ethyleneterephthalate] (PET, commercial name:…”

Section: Current Medical Treatmentsmentioning

confidence: 99%

“…To address the biocompatibility and bioactive limitations of synthetic scaffolds, other groups have attempted to use biological scaffolds, including extracted collagen and decellularized porcine carotids [12,30,31]. While these scaffolds do offer improved compatibility and biological compliance, they tend to be weak, difficult to handle, and inconsistent.…”

Section: Synthetic Graftsmentioning

confidence: 99%

“…To address the biocompatibility and bioactive limitations of synthetic scaffolds, many groups have attempted to use biological scaffolds, including extracted collagen and decellularized porcine carotids [12,30]. Typically, biological scaffolds are chosen as tissue engineering scaffolds for improved compatibility and biological compliance, however, these scaffolds tend to be weak or difficult to handle, and therefore, many groups rely on the customizability and repeatability of synthetic materials.…”

Section: Appendix K Alternative Biological Scaffolds Introductionmentioning

confidence: 99%

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Development of an In-Vitro Tissue Engineered Blood Vessel Mimic Using Human Large Vessel Cell Sources

Delagrammaticas¹

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DEVELOPMENT OF AN IN-VITRO TISSUE ENGINEERED BLOODHUVECs were incubated with Cell Tracker™ prior to dual sodding and both cell types with bisbenzimide after graft harvest to attempt to distinguish between cell types. Results from the thesis illustrate that large vessel smooth muscle and endothelial cells can be sodded onto ePTFE scaffolds and sustained within the in-vitro BVM system for up to 7 days. Furthermore, cost analysis demonstrates that the addition of a smooth muscle cell layer adds minimal costs to the BVM system. In conclusion, the studies contained within this thesis culminate in a protocol for the dual sodding of smooth muscle and endothelial cells with the aim of creating a physiologically representative co-culture blood vessel mimic.vi ACKNOWLEDGMENTS

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3D Human iPSC Blood Vessel Organoids as a Source of Flow‐Adaptive Vascular Cells for Creating a Human‐Relevant 3D‐Scaffold Based Macrovessel Model

et al. 2022

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Abstract3D‐scaffold based in vitro human tissue models accelerate disease studies and screening of pharmaceutics while improving the clinical translation of findings. Here is reported the use of human induced pluripotent stem cell (hiPSC)‐derived vascular organoid cells as a new cell source for the creation of an electrospun polycaprolactone‐bisurea (PCL‐BU) 3D‐scaffold‐based, perfused human macrovessel model. A separation protocol is developed to obtain monocultures of organoid‐derived endothelial cells (ODECs) and mural cells (ODMCs) from hiPSC vascular organoids. Shear stress responses of ODECs versus HUVECs and barrier function (by trans endothelial electrical resistance) are measured. PCL‐BU scaffolds are seeded with ODECs and ODMCs, and tissue organization and flow adaptation are evaluated in a perfused bioreactor system. ODECs and ODMCs harvested from vascular organoids can be cryopreserved and expanded without loss of cell purity and proliferative capacity. ODECs are shear stress responsive and establish a functional barrier that self‐restores after the thrombin challenge. Static bioreactor culture of ODECs/ODMCs seeded scaffolds results in a biomimetic vascular bi‐layer hierarchy, which is preserved under laminar flow similar to scaffolds seeded with primary vascular cells. HiPSC‐derived vascular organoids can be used as a source of functional, flow‐adaptive vascular cells for the creation of 3D‐scaffold based human macrovascular models.

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Coculture of endothelial and smooth muscle cells on a collagen membrane in the development of a small-diameter vascular graft

Cited by 84 publications

References 21 publications

A Strategy for Depositing Different Types of Cells in Three Dimensions to Mimic Tubular Structures in Tissues

A Strategy for Depositing Different Types of Cells in Three Dimensions to Mimic Tubular Structures in Tissues

Development of an In-Vitro Tissue Engineered Blood Vessel Mimic Using Human Large Vessel Cell Sources

3D Human iPSC Blood Vessel Organoids as a Source of Flow‐Adaptive Vascular Cells for Creating a Human‐Relevant 3D‐Scaffold Based Macrovessel Model

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