Coculture of ovine zygotes fertilized in vivo or in vitro and positive effect of CZB medium on the development of in vitro fertilized zygotes

Bernardi, Mari Lourdes; Delouis, Claude

doi:10.1051/rnd:19950410

Cited by 3 publications

(3 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For example, maternal administration of progesterone in the very early stages of pregnancy [2], nuclear cloning of embryos [3], and in vitro culture [4,5] and coculture of embryos on a granulosa cell layer [6][7][8] all seem to affect fetal growth. In addition, the transfer of embryos to a temporary advanced uterine environment (asynchronous transfer) has been shown to increase crown-to-rump length by approximately 40% at 37 days of gestation [9] or to increase weight by 42% at 21 days of gestation [10].…”

Section: Introductionmentioning

confidence: 99%

Temporary Exposure of Ovine Embryos to an Advanced Uterine Environment Does Not Affect Fetal Weight but Alters Fetal Muscle Development1

Maxfield¹,

Sinclair²,

Dunne³

et al. 1998

Biology of Reproduction

View full text Add to dashboard Cite

Embryo transfer techniques may result in fetuses that are heavier at birth and that have been described as highly muscled. The aim of this study was to investigate myogenesis in lambs derived from embryo transfer. Embryos were transferred at Day 3 (estrus = Day 0) to a 3 days-advanced uterine environment, maintained there for 3 days, recovered, and then returned to a synchronous (Day 6) uterus; these fetuses comprised the asynchronous group. Control animals were created by synchronous embryo recovery and single transfer at Day 3. Asynchronous transfer did not affect fetal weight or curved crown-rump length between 46 and 135 days of gestation. No differences were detected between groups at Days 110-135 with respect to muscle mass or protein, RNA, and DNA content. However, total muscle fiber number was significantly increased in plantaris muscles from the asynchronous groups at Day 110 and Day 125, suggestive of prolonged hyperplasia. In addition, the levels of Myf 5 protein and the secondary-to-primary fiber ratio were altered in plantaris muscle from the asynchronous group. The growth data are in contrast to previously reported findings. The results show that fetal myogenesis can be altered by very early events in embryogenesis and suggest that any inferences made solely on the basis of fetal or muscle weight may be fallacious.

show abstract

Section: Introductionmentioning

confidence: 99%

Temporary Exposure of Ovine Embryos to an Advanced Uterine Environment Does Not Affect Fetal Weight but Alters Fetal Muscle Development1

Maxfield¹,

Sinclair²,

Dunne³

et al. 1998

Biology of Reproduction

View full text Add to dashboard Cite

show abstract

“…Pelo fato desse bloqueio não ocorrer se os zigotos fossem incubados no oviduto de ovelhas [16,17], esse sistema foi preferencialmente utilizado, nos primeiros trabalhos de FIV em ovinos. Entretanto, um efeito benéfico do cocultivo sobre monocamadas de células epiteliais do oviduto foi observado com zigotos ovinos oriundos de maturação e fecundação in vivo [24], o que foi confirmado, posteriormente, para embriões ovinos produzidos in vitro [9,80].…”

Section: Cultivo Dos Embriõesunclassified

“…A presença de glicose no meio de cultivo é considerada um dos fatores que aumentam a fragmentação de zigotos ovinos quando cultivados in vitro [51]. Além de ter sido favorável para o cultivo de zigotos ovinos fecundados in vivo [44], redução da fragmentação e maior proporção de blastocistos foi obtida quando zigotos ovinos produzidos in vitro foram cocultivados durante as primeiras 48h no meio CZB, o qual não contém glicose [9].…”

Section: Efeito Da Glicoseunclassified

Produção in vitro de embriões ovinos

Bernardi

2018

Acta Scientiae. Vet.

View full text Add to dashboard Cite

A produção in vitro (PIV) de embriões compreende as etapas de maturação dos oócitos, capacitação dos espermatozóides, fecundação dos oócitos e cultivo dos zigotos até o estádio de mórula ou blastocisto. Embora a execução destas etapas não seja complexa, vários fatores podem interferir no seu sucesso. Se uma das etapas não é realizada corretamente, uma redução do índice de sucesso na etapa seguinte pode ocorrer. Nesta revisão são detalhadas as etapas referentes à PIV de embriões ovinos, sendo enfocados a eficiência de cada uma e os avanços obtidos. Também são enfatizados os aspectos que devem ser melhorados para que as tecnologias associadas à PIV de embriões ovinos tenha uma aplicação mais difundida.

show abstract

Sex-related differences in the developmental rate of in-vitro matured/in-vitro fertilized ovine embryos

Bernardi¹,

Delouis²

1996

Human Reproduction

View full text Add to dashboard Cite

Sex determination of in-vitro matured/in-vitro fertilized ovine embryos cultured in synthetic oviduct fluid (SOF) medium was performed by the polymerase chain reaction amplification of specific Y DNA sequences so as to test the influence of sex on developmental growth during the preimplantation period. At 144 h post-insemination, embryos with a blastocoel were classified as the fast-developing group, whereas those showing a blastocoel only after this length of time were classified as the slow-developing group. At 144 h post-insemination, fast-developing embryos were cultured separately and some were classified according to the size of their blastocoel. At the end of culture (207 h post-insemination), all embryos were classified according to both their developmental stage and their morphological quality. The male:female sex ratio of fast-developing embryos was significantly higher than the expected ratio of 50%. More males were observed at the most advanced developmental stage at both 144 and 207 h post-insemination. The proportion of males did not differ between the good- and poor-quality groups, although a skewed sex ratio was observed with embryos of better quality at the most developed stage. In conclusion, embryos at the most developed stage were predominantly male and were derived mainly from the fast-developing group, raising the possibility of a deviation in the sex ratio after the transfer of in-vitro matured/in-vitro fertilized ovine embryos.

show abstract

Coculture of ovine zygotes fertilized in vivo or in vitro and positive effect of CZB medium on the development of in vitro fertilized zygotes

Cited by 3 publications

References 35 publications

Temporary Exposure of Ovine Embryos to an Advanced Uterine Environment Does Not Affect Fetal Weight but Alters Fetal Muscle Development1

Temporary Exposure of Ovine Embryos to an Advanced Uterine Environment Does Not Affect Fetal Weight but Alters Fetal Muscle Development1

Produção in vitro de embriões ovinos

Sex-related differences in the developmental rate of in-vitro matured/in-vitro fertilized ovine embryos

Contact Info

Product

Resources

About