Coculturing cumulus oocyte complexes with denuded oocytes alters zona pellucida ultrastructure in in vitro matured bovine oocytes

Effect of peroxiredoxin II on the quality and mitochondrial activity of pre-implantation bovine embryos

“…This result is in agreement with our earlier studies performed in our laboratory that highlighted the crucial role of IVM step in in vitro embryo production Choi et al, 2013). Improving the microenvironment of oocytes during IVM either by oocyte-secreted factors derived from denuded oocytes Choi et al, 2013) cultures or addition of 9-cis retinoic acid (Deb et al, 2011 has enhanced developmental rate and embryo quality.…”

“…This result is in agreement with our earlier studies performed in our laboratory that highlighted the crucial role of IVM step in in vitro embryo production Choi et al, 2013). Improving the microenvironment of oocytes during IVM either by oocyte-secreted factors derived from denuded oocytes Choi et al, 2013) cultures or addition of 9-cis retinoic acid (Deb et al, 2011 has enhanced developmental rate and embryo quality. Interestingly, induction of intracellular GSH synthesis via supplementation of IVM medium with antioxidants (␤-mercaptoethanol, cystine and cysteine) has improved in vitro embryo development (de Matos and Furnus, 2000) and quality (Furnus et al, 2008).…”

“…In the present study, although mechanism by which this occurred was not fully investigated, it may be that PRDX II treatment as antioxidant enzyme maintains the mitochondrial function by removing ROS, and produces "healthy" matured oocytes, result in improved blastocyst quality. Therefore, it is speculated from our results and those reported previously (Albuz et al, 2010;Deb et al, 2012;Dey et al, 2012;Choi et al, 2013) that improving IVM condition could enable the embryo to bypass the post-fertilization in vitro conditions. Although our data partially support this idea, further investigations using global profiling tool like microarray could help more to understand the molecular mechanisms involved in pronounced improving embryo quality.…”

Oocyte maturation with royal jelly increases embryo development and reduces apoptosis in goats

“…The synergistic effect of royal jelly on embryo development could be attributed to improving the IVM microenvironment (Choi et al, 2013), in addition to increasing nuclear and cytoplasmic maturation of oocytes (Dey et al, 2012). Interestingly, it seems that the royal jelly antioxidative potentiality could also be sustained throughout embryo cleavage until blastocyst formation, as observed in the current study and previous reports when various antioxidant supplements were used during IVM (Ali et al, 2003;Rodríguez-González et al, 2003;Kwak et al, 2012;Do et al, 2015;Fakruzzaman et al, 2015;Mishra et al, 2016).…”

“…There are different ways to improve the oocyte microenvironment during the course of the maturation process. Co-culture of intact COCs with a specific ratio of denuded oocytes has improved nuclear, cytoplasmic maturation (Dey et al, 2012;Souza-Fabjan et al, 2016) and the rate of blastocyst formation, in addition to quality (Choi et al, 2013). Antioxidants such as peroxiredoxin II (Fakruzzaman et al, 2015), melatonin (Do et al, 2015), resveratrol (Kwak et al, 2012), l-carnitine 131 (Mishra et al, 2016), cysteine (Ali et al, 2003), and cysteamine (Rodríguez-González et al, 2003) have been supplemented to IVM media to improve developmental competence of pre-implantation blastocysts of different mammalian species.…”