2022
DOI: 10.1038/s41598-022-17442-2
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Coculturing of Mucor plumbeus and Bacillus subtilis bacterium as an efficient fermentation strategy to enhance fungal lipid and gamma-linolenic acid (GLA) production

Abstract: This study aimed to improve lipid and gamma-linolenic acid (GLA) production of an oleaginous fungus, Mucor plumbeus, through coculturing with Bacillus subtilis bacteria, optimising the environmental and nutritional culture conditions, and scaling them for batch fermentation. The maximum levels of biomass, lipid, fatty acid, and GLA in a 5 L bioreactor containing cellobiose and ammonium sulfate as the optimal carbon and nitrogen sources, respectively, achieved during the coculturing processes were 14.5 ± 0.4 g/… Show more

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Cited by 8 publications
(3 citation statements)
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“…It is worth mentioning that the optimal concentrations of corn steep powder and yeast extract were determined to be 4-6 times greater than their initial levels. These nitrogen source concentrations were notably higher than those commonly employed in the medium for the production of other chemicals by B. subtilis, as documented in previous studies [31][32][33][34][35]. This finding implies that a higher nitrogen input during the fermentation process is necessary to attain enhanced riboflavin production by the strain.…”
Section: Discussionsupporting
confidence: 73%
“…It is worth mentioning that the optimal concentrations of corn steep powder and yeast extract were determined to be 4-6 times greater than their initial levels. These nitrogen source concentrations were notably higher than those commonly employed in the medium for the production of other chemicals by B. subtilis, as documented in previous studies [31][32][33][34][35]. This finding implies that a higher nitrogen input during the fermentation process is necessary to attain enhanced riboflavin production by the strain.…”
Section: Discussionsupporting
confidence: 73%
“…The most effective fungal isolates were subjected to phylogenetic analysis by ITS region sequencing, which molecularly identified them as A. nidulans AUMC 15444 and A. terreus AUMC 15447. The ITS regions represent important efficient markers for confirming fungal strain identification at the species level [102]. Molecular identification of Aspergillus strains at the species level using ITS region sequencing was presented as an efficient substitute approach for their accurate identification [103].…”
Section: Discussionmentioning
confidence: 99%
“…The composition of primrose oil, characterized by its richness in GLA, signifies its potential role as a natural source for fostering healthy skin and contributing to the intricate balance of hormones within the body. 23…”
Section: Essential Fatty Acidsmentioning
confidence: 99%