Codelivery to Mammalian Cells of a Transcriptional Factor with cis-Acting Element Using Cationic Liposomes

Farhood, Hassan; Gao, Xiang; Barsoum, James; Huang, Leaf

doi:10.1006/abio.1995.1112

Cited by 12 publications

(4 citation statements)

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“…Cationic liposomes have been shown to co-deliver transcriptional factor with plasmid DNA containing the transcription factor response element (28,30). The plasmid DNA-protein complexes were transfected into cells using cationic lipid-mediated transfection protocols.…”

Section: Discussionmentioning

confidence: 99%

“…The plasmid DNA-protein complexes were transfected into cells using cationic lipid-mediated transfection protocols. However, the delivery efficiency was very low, required chloroquine treatment (28) and the benefit of using the cationic lipid was unclear since the protein alone can spontaneously enter the cell without the aid of any delivery system (30,32). Cationic lipids have also been used to deliver protein into the intracellular processing pathway leading to antigen presentation, but the discrimination between inside and outside antigen presentation was difficult and the efficiency of functional intracellular protein delivery remains unclear (29).…”

Section: Discussionmentioning

confidence: 99%

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Intracellular Delivery of Proteins with a New Lipid-mediated Delivery System

Zelphati¹,

Wang²,

Kitada³

et al. 2001

Journal of Biological Chemistry

209

180

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There are many very effective methods to introduce transcriptionally active DNA into viable cells but approaches to deliver functional proteins are limited. We have developed a lipid-mediated delivery system that can deliver functional proteins or other bioactive molecules into living cells. This delivery system is composed of a new trifluoroacetylated lipopolyamine (TFA-DODAPL) and dioleoyl phosphatidylethanolamine (DOPE). This cationic formulation successfully delivered antibodies, dextran sulfates, phycobiliproteins, albumin, and enzymes (␤-galactosidase and proteases) into the cytoplasm of numerous adherent and suspension cells. Two systems were used to demonstrate that the proteins were delivered in a functionally active form. First, intracellular ␤-galactosidase activity was clearly demonstrated within X-gal-stained cells after TFA-DODAPL:DOPE-mediated delivery of the enzyme. Second, the delivery system mediated delivery of several caspases (caspase 3, caspase 8, and granzyme B) into cultured cell lines and primary cells triggering apoptosis. Mechanistic studies showed that up to 100% of the protein mixed with the lipid formulation was captured into a lipid-protein complex, and up to 50% of the input protein associated with cells. This lipid-mediated transport system makes protein delivery into cultured cells as convenient, effective, and reliable as DNA transfection.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Intracellular Delivery of Proteins with a New Lipid-mediated Delivery System

Zelphati¹,

Wang²,

Kitada³

et al. 2001

Journal of Biological Chemistry

209

180

View full text Add to dashboard Cite

show abstract

“…The cholesterol-based cationic lipid has been used as the major hydrophobic domain of liposomes for gene delivery due to being less toxic than other cationic lipids [ 16 ]. Many cholesterol-based cationic lipids have been synthesized, among which 3β-[ N -( N ', N '-dimethylaminoethyl)-carbamoyl] cholesterol (DC-Chol, Figure 1 ) showed efficient transfection in gene delivery and was used as the commercially available liposome reagent [ 17 , 18 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Novel Cholesterol-Based Cationic Lipids as Transfecting Agents of DNA for Efficient Gene Delivery

Huan

Wan

et al. 2015

IJMS

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The design, synthesis and biological evaluation of the cationic lipid gene delivery vectors based on cholesterol and natural amino acids lysine or histidine are described. Cationic liposomes composed of the newly synthesized cationic lipids 1a or 1b and neutral lipid DOPE (1,2-dioleoyl-l-α-glycero-3-phosphatidyl-ethanolamine) exhibited good transfection efficiency. pEGFP-N1 plasmid DNA was transferred into 293T cells by cationic liposomes formed from cationic lipids 1a and 1b, and the transfection activity of the cationic lipids was superior (1a) or parallel (1b) to that of the commercially available 3β-[N-(N',N'-dimethylaminoethyl)-carbamoyl] cholesterol (DC-Chol) derived from the same cholesterol backbone with different head groups. Combined with the results of agarose gel electrophoresis, transfection experiments with various molar ratios of the cationic lipids and DOPE and N/P (+/−) molar charge ratios, a more effective formulation was formed, which could lead to relatively high transfection efficiency. Cationic lipid 1a represents a potential agent for the liposome used in gene delivery due to low cytotoxicity and impressive gene transfection activity.

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“…In suicide gene therapy, transgene expression is selectively confined to malignant cells by targeting at the transcriptional level. Therefore, cis-acting sequences are designed to limit transgene expression to target cells using transcriptional control elements that drive transgene expression in malignant cells (4,49,50) (Table 7). With regard to exogenous control of gene expression subsequent to transduction, an inducer or repressor acts on it synthetic transcription factor that recognizes mol.ifs unique to the promoter of the transgene.…”

Section: Transcriptionally Targeted and Regulatable Vectoismentioning

confidence: 99%

Strategy of liver‐directed gene therapy: present status and future prospects

Shiratori

Kanai

Ohashi

et al. 1999

Liver

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: The liver is particularly amenable to gene therapy as it is the site of many metabolic diseases and malignancies. Thus, liver‐directed gene therapy is being actively pursued and developed as a method of treatment for various liver diseases. Strategies of liver‐directed gene therapy include drug delivery to the liver, compensation of the defective gene(s), anti‐tumor activity, anti‐viral therapy, and immunomodulation. The strategy chosen for liver‐directed gene therapy depends on the genetic basis of the disease. Many aspects are key factors to the success of the chosen strategy: intervention of genes, efficient gene delivery system, stable transgene expression, transgene regulation, target cell transfection, and timing of transgene expression. Several tactics can be used to overcome problems in the above, and these include the use of a gene switch to exogenously regulate transgene expression, targeting at the transcriptional level, circumvention of the immune response (as in the use of adenovirus vector to achieve long‐term correction of genetic diseases), and genetically engineered antibodies in gene transfer. At the present rate of research activity and development, gene therapies may soon be more efficient than current standard treatments for some liver diseases.

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Codelivery to Mammalian Cells of a Transcriptional Factor with cis-Acting Element Using Cationic Liposomes

Cited by 12 publications

References 0 publications

Intracellular Delivery of Proteins with a New Lipid-mediated Delivery System

Intracellular Delivery of Proteins with a New Lipid-mediated Delivery System

Novel Cholesterol-Based Cationic Lipids as Transfecting Agents of DNA for Efficient Gene Delivery

Strategy of liver‐directed gene therapy: present status and future prospects

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