Coencapsulation of Carbon Dots and Gold Nanoparticles over <i>Escherichia coli</i> for Bacterium Assay by Surface-Enhanced Raman Scattering

Anchustegui, Victoria Arantza León; Zhu, Junhao; He, Luanying; Bi, Ying; Dong, Yiyang; Liu, Jiahui; Wang, Shihui

doi:10.1021/acsabm.0c01154

Cited by 7 publications

(2 citation statements)

References 32 publications

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“…Results supported aptness of CDs as bioimaging nanomaterial for bacterial systems. Imparting fluorescence to microorganisms by CDs would facilitate their detection, analysis, quantitation and tracking for different identification, and theranostic purposes ( León Anchustegui et al, 2021 ; Hallaji et al, 2022 ).…”

Section: Resultsmentioning

confidence: 99%

Carbon dots labeled Lactiplantibacillus plantarum: a fluorescent multifunctional biocarrier for anticancer drug delivery

Abdullah

Mahmoud

El-Nikhely

et al. 2023

Front. Bioeng. Biotechnol.

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A carbon dots (CDs)-biolabeled heat-inactivated Lactiplantibacillus plantarum (HILP) hybrid was investigated as a multifunctional probiotic drug carrier with bioimaging properties using prodigiosin (PG) as anticancer agent. HILP, CDs and PG were prepared and characterized using standard methods. CDs-labeled HILP (CDs/HILP) and PG loaded CDs/HILP were characterized by transmission electron microscopy (TEM), laser scanning confocal microscopy (LSCM) and for entrapment efficiency (EE%) of CDs and PG, respectively. PG-CDs/HILP was examined for stability and PG release. the anticancer activity of PG-CDs/HILP was assessed using different methods. CDs imparted green fluorescence to HILP cells and induced their aggregation. HILP internalized CDs via membrane proteins, forming a biostructure with retained fluorescence in PBS for 3 months at 4°C. Loading PG into CDs/HILP generated a stable green/red bicolor fluorescent combination permitting tracking of both drug carrier and cargo. Cytotoxicity assay using Caco-2 and A549 cells revealed enhanced PG activity by CDs/HILP. LCSM imaging of PG-CDs/HILP-treated Caco-2 cells demonstrated improved cytoplasmic and nuclear distribution of PG and nuclear delivery of CDs. CDs/HILP promoted PG-induced late apoptosis of Caco-2 cells and reduced their migratory ability as affirmed by flow cytometry and scratch assay, respectively. Molecular docking indicated PG interaction with mitogenic molecules involved in cell proliferation and growth regulation. Thus, CDs/HILP offers great promise as an innovative multifunctional nanobiotechnological biocarrier for anticancer drug delivery. This hybrid delivery vehicle merges the physiological activity, cytocompatibility, biotargetability and sustainability of probiotics and the bioimaging and therapeutic potential of CDs.

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Section: Resultsmentioning

confidence: 99%

Carbon dots labeled Lactiplantibacillus plantarum: a fluorescent multifunctional biocarrier for anticancer drug delivery

Abdullah

Mahmoud

El-Nikhely

et al. 2023

Front. Bioeng. Biotechnol.

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“…coli, which often causes food safety issues and sepsis. Thus, it is essential to rapidly detect these two bacteria in the environment using SERS technology. − In this research, S. H. and E. coli were cultivated separately at an OD of 0.6 and then were rinsed three times using DI water.…”

Section: Resultsmentioning

confidence: 99%

Hydrophilic–Hydrophobic Nanohybrids of AuNP-Immobilized Two-Dimensional Nanomica Platelets as Flexible Substrates for High-Efficiency and High-Selectivity Surface-Enhanced Raman Scattering Microbe Detection

Chen

Wang

Chang

et al. 2022

ACS Appl. Bio Mater.

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A flexible hybrid substrate was developed by affixing gold nanoparticles (AuNPs) onto the surface of two-dimensional nanomica platelets (NMPs). The substrate was successfully used in biosensors with high efficiency and high selectivity through surface-enhanced Raman scattering (SERS). By controlling the amphiphilicity of the hybrid substrate, the flexible substrate was made highly selective toward biomolecules. Four different SERS substrate systems were constructed, including intercalated mica, exfoliated NMPs, hydrophilic exfoliated NMPs, and hydrophobic exfoliated NMPs. NMPs were only 1 nm thick. AuNPs adsorbed on both sides of NMPs and thus created excellent threedimensional hot junction effects in the z-axis direction. For the detection of adenine in DNA, a satisfactory Raman enhancement factor (EF) of up to 8.9 × 10 6 was achieved with the detection limit as low as 10 −8 M. Subsequently, the AuNP/NMP hybrids were adopted to rapidly detect hydrophilic Staphylococcus hominis and hydrophobic Escherichia coli. The AuNP/PIB−POE−PIB/NMP nanohybrid was concurrently hydrophilic and hydrophobic. This amphiphilic property greatly enhanced the detection selectivity and signal intensity for hydrophilic or hydrophobic bacteria. Overall, AuNPs/PIB−POE−PIB/NMPs developed as SERS substrates enable rapid, sensitive biodetection.

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Green synthesis of carbon dots-functionalized silver nanoparticles for SERS-based detection of thiram fungicide

Gokulakrishnan,

Koppole,

Rugmini

et al. 2023

Appl. Phys. A

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Coencapsulation of Carbon Dots and Gold Nanoparticles over Escherichia coli for Bacterium Assay by Surface-Enhanced Raman Scattering

Cited by 7 publications

References 32 publications

Carbon dots labeled Lactiplantibacillus plantarum: a fluorescent multifunctional biocarrier for anticancer drug delivery

Carbon dots labeled Lactiplantibacillus plantarum: a fluorescent multifunctional biocarrier for anticancer drug delivery

Hydrophilic–Hydrophobic Nanohybrids of AuNP-Immobilized Two-Dimensional Nanomica Platelets as Flexible Substrates for High-Efficiency and High-Selectivity Surface-Enhanced Raman Scattering Microbe Detection

Green synthesis of carbon dots-functionalized silver nanoparticles for SERS-based detection of thiram fungicide

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