Coenzyme Q₁₀ protects against oxidative stress‐induced cell death and enhances the synthesis of basement membrane components in dermal and epidermal cells

Abstract

Coenzyme Q10 (CoQ10), which has both energizing and anti-oxidative effects, is also reported to have antiaging action, e.g., reducing the area of facial wrinkles. However, the mechanism of its anti-aging activity is not fully established. Here, we examined the effect of CoQ10 on human dermal and epidermal cells. CoQ10 promoted proliferation of fibroblasts but not keratinocytes. It also accelerated production of basement membrane components, i.e., laminin 332 and type IV and VII collagens, in keratinocytes and fibroblasts, respectively; however, it had no effect on type I collagen production in fibroblasts. CoQ10 also showed protective effects against cell death induced by several reactive oxygen species in keratinocytes, but only when its cellular absorption was enhanced by pretreatment of the cells with highly CoQ10-loaded serum. These results suggest that protection of epidermis against oxidative stress and enhancement of production of epidermal basement membrane components may be involved in the antiaging properties of CoQ10 in skin.

Hexane Fraction of Melandrium firmum Extract Induces Laminin-332 Expression in Human Keratinocyte

“…그 이외에 표피와 진피에 존재하는 서로 다른 세포 사 이나 세포와 세포외기질(extracellular matrix, ECM) 사 이의 물리적인 장벽 역할, 표피와 진피 두 층 간의 선 택적인 투과 기능 및 커뮤니케이션의 조절, 그리고 세 포의 기능을 조절하는 등 다양한 역할을 수행한다 [4][5][6][7][8][9][10]. 피부 기저막을 구성하고 있는 주요성분으로는 콜라겐 타입 IV, laminin-332 (LN-332), 콜라겐 타입 VII, 퍼레칸(perlecan), 니도겐(nidogen) 등이며, 이런 성 분들이 서로 그물 형태의 구조를 형성하고 있다 [11,12].…”

“…피부 주름의 증가와 탄력 감 소는 진피 내에 존재하는 세포외기질의 변형 및 감소 로 인해 발생된다는 사실은 잘 알려져 있으나 표피와 진피 사이에 존재하는 기저막의 구조적, 기능적 변화 또한 피부 노화에 관여한다고 알려져 있다 [5,6,11].…”

“…이러한 피부 기저막 구성성분의 분해 및 생성 감소에 따른 피 부 기저막의 손상은 피부에서의 주름 형성이나 탄력 저하 등과 같은 피부 노화의 직접적인 원인 중 하나라 고 여겨지고 있다. 따라서 피부 노화를 예방하는 방법 의 하나로 피부 기저막을 구성하고 있는 성분들의 발 현을 증가시키는 물질들을 발굴하고자 하는 노력들이 다수 보고되고 있다 [5,6,11,12,18,19].…”

A comparative evaluation of coenzyme Q10-loaded liposomes and solid lipid nanoparticles as dermal antioxidant carriers

“…It has an important role in generating adenosine triphosphate during aerobic cellular respiration and inhibits cell membrane peroxidation in the reduced form. 4,5 However, to deliver Q10 to deeper layers of skin is challenging due to the low aqueous solubility of Q10 and due to the barrier function of stratum corneum. 6 As lipidic nanocarriers, liposomes and solid lipid nanoparticles (SLN) have taken the interest of scientists for their potential to overcome the topical drug delivery challenges.…”

Development and evaluation of coenzyme Q10 loaded solid lipid nanoparticle hydrogel for enhanced dermal delivery

“…Coenzyme Q10 (Q10) is the only lipid soluble endogenous antioxidant. It supports ATP generation during aerobic cellular respiration in the electron transport chain in the mitochondria and inhibits cell membrane peroxidation in reduced form (6,7). However, it is very difficult to prepare a formulation that helps Q10 delivery to deeper layers of the skin due to the low aqueous solubility of Q10 (log P >10) and due to the barrier function of stratum corneum (8).…”