Coenzyme Q10 concentrations and antioxidant status in tissues of breast cancer patients

Portakal, Oytun; Özkaya, Özay; İnal, Mine; Bozan, Berrin; Koşan, MüBerra; Sayek, İskender

doi:10.1016/s0009-9120(00)00067-9

Cited by 165 publications

(128 citation statements)

References 30 publications

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“…1,2 Recent clinical and experimental findings suggest that oxidative stress is associated with the progression of breast cancer. [3][4][5][6] Oxidative damage of DNA has been implicated in the initiation of mammary tumors. [7][8][9] Aside from the role of oxidants in the induction of mutation, oxidative stress also mediates cell signaling pathways involved with increasing mutation rate, activating growth-promoting signaling pathways, stimulating tumor angiogenesis, and accelerating tumor progression.…”

Section: Introductionmentioning

confidence: 99%

Oral administration of Pyrrolidine Dithiocarbamate (PDTC) inhibits VEGF expression, tumor angiogenesis, and growth of breast cancer in female mice

Young²,

Busby³

et al. 2009

Cancer Biology & Therapy

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The progression of breast cancer is associated with oxidative stress. However, the effects of pyrrolidine dithiocarbamate (PDTC), a known antioxidant, on the development of breast cancer are poorly understood. The present study evaluates the effects of PDTC on tumor growth, the expression of vascular endothelial growth factor (VEGF) and angiogenesis of breast cancer in female mice. Eight week old female mice (C57BL/6J) were given PDTC at 100 to 200 mg/kg/day for three weeks (n = 10). The control mice received regular drinking water only. In the second week, 5 x 10 5 E0771 (mouse breast cancer) cells were injected in the pad of the fourth mammary gland of the mice. Tumor size was monitored using dial calipers. At the end of the experiment, the tumors were isolated and measured for tumor size, intratumoral microvessel (IM) density using CD31 immunohistochemistry staining, NFκB activation using EMSA, and VEGF protein levels using ELISA. PDTC treatment caused a significant decrease in tumor weight compared to the control (0.64 ± 0.22 vs. 1.43 ± 0.31 g; n = 10; p < 0.01) and a significant decrease in IM density (66.1 ± 5.3 vs. 84.2 ± 9.4 IM# /mm 2 ; p < 0.01). There was a significant decrease in tissue protein levels of VEGF (22.6 ± 2.1 vs. 32.4 ± 2.6 pg/mg) and a 43% reduction of NFκB activation in the breast tumors of mice treated with PDTC compared to the control group (p < 0.01). Western blot indicated that estrogen receptor-a (ERa), VEGF receptor-1 (Flt-1) and VEGF receptor-2 (Flk-1) were expressed in E0771 cells. VEGF receptor inhibitor SU5416 and PDTC synergistically suppressed the proliferation of E0771 cells. PDTC also significantly inhibited the migration of cultured E0771 cells. These results support the hypothesis that PDTC suppresses tumor angiogenesis, growth and migration of breast cancer via inhibiting autocrine and paracrine effects of VEGF through the reduction of NFκB activation and VEGF expression.

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Section: Introductionmentioning

confidence: 99%

Oral administration of Pyrrolidine Dithiocarbamate (PDTC) inhibits VEGF expression, tumor angiogenesis, and growth of breast cancer in female mice

Young²,

Busby³

et al. 2009

Cancer Biology & Therapy

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“…An increase in free radical generation or a decrease in antioxidant levels in living organisms, or both, suggest that these factors play a critical role in the etiology of carcinogenesis [27]. Genetic variation was also considered a biomarker of susceptibility.…”

Section: Discussionmentioning

confidence: 99%

Role of CYP2E1 and NQO1 polymorphisms in oxidative stress derived cancer in Thais with and without dyslipidemia

et al. 2015

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Background: Hyperlipidemia can induce the endogenous production of reactive oxygen species (ROS), which may cause carcinogenesis. Cytochrome P450 (CYP)2E1 activity, induced by various factors including polyunsaturated fatty acids, effects the incidence of cancers, whereas NQO1, a flavoprotein, may protect against ROS. Objectives: To investigate the effect of CYP2E1 and NQO1 polymorphism on oxidative stress status in Thais with and without dyslipidemia. Methods: We included 1380 apparently healthy employees of the Electricity Generating Authority of Thailand in this study. We determined their CYP2E1 and NQO1 genotypes and related these to blood lipid profiles, and circulating levels of antioxidant enzymes, malondialdehyde (MDA), and reduced glutathione (GSH). Lifestylerelated factors were determined from questionnaires. Results: All tested genotype frequencies were in Hardy-Weinberg equilibrium. The heterozygous and variant genotype distribution and allele frequency of CYP2E1*5B were less common than CYP2E1*6. Heterozygous NQO1 was the most prevalent form. The frequency of the mutated allele CYP2E1*5B was 0.16, CYP2E1*6 was 0.22, and NQO1*2 was 0.43. Significant differences were observed for blood cholesterol, triglyceride, low-density lipoprotein-cholesterol, and high-density lipoprotein-cholesterol between normolipidemic participants, and those with hypercholesterolemia, hypertriglyceridemia, and combined hyperlipidemia. Participants in the hyperlipidemic subgroup who bore any variant alleles of genes had higher plasma MDA and GSH levels, and superoxide dismutase and glutathione peroxidase activity, but lower catalase activity when compared with normolipidemic participants bearing wild-type alleles. Conclusions: Variations in genetic disposition and dyslipidemia can modify oxidative stress status. Relatively more free radicals may be generated in individuals in subgroups with hyperlipidemia bearing any variant alleles.

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“…First, aggregating results of different models is a reasonable first step, but it is unclear how sensitive the results are to the assumptions of individual models and whether it is reasonable to aggregate possibly heterogeneous findings-with different populations and modeling approaches-into an overall cost-effectiveness ratio. Many previous studies (e.g., Vegter et al 4 ) have noted the heterogeneity found across cost-effectiveness analyses of genetic testing. In future research, it would be helpful to develop a transparent means of aggregating results so that they can be readily replicated.…”

Section: Key Emerging Themes For Assessing the Cost-effectiveness Of mentioning

confidence: 99%

“…Furthermore, abnormally low plasma concentrations of CoQ10 have been found in a number of cancer types, including cervical cancer, breast cancer, and melanoma. 4 Although intensive research is needed to further explore the underlying mechanisms, increasing data have strongly indicated an undetermined implication of CoQ10 biosynthesis gene mutations in carcinogenesis.…”

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confidence: 99%

Response to Trevisson et al.

Zhang

Lin

Chiu

et al. 2015

Genetics in Medicine

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Coenzyme Q10 concentrations and antioxidant status in tissues of breast cancer patients

Cited by 165 publications

References 30 publications

Oral administration of Pyrrolidine Dithiocarbamate (PDTC) inhibits VEGF expression, tumor angiogenesis, and growth of breast cancer in female mice

Oral administration of Pyrrolidine Dithiocarbamate (PDTC) inhibits VEGF expression, tumor angiogenesis, and growth of breast cancer in female mice

Role of CYP2E1 and NQO1 polymorphisms in oxidative stress derived cancer in Thais with and without dyslipidemia

Response to Trevisson et al.

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