Coenzyme Q10 Prevents the Interleukin‐1 Beta Induced Inflammatory Response via Inhibition of MAPK Signaling Pathways in Rat Articular Chondrocytes

Abstract

Preclinical Research Osteoarthritis (OA) is a widely prevalent degenerative joint disease that severely impairs the health of the elderly population resulting in a heavy economic burden worldwide. Coenzyme Q10 (CoQ10) has shown anti-inflammatory effects in some diseases. The present study aimed to investigate if CoQ10 would suppress catabolic responses of interleukin (IL)-1β-induced chondrocytes. Rat chondrocytes were cultured and pretreated with CoQ10, and then stimulated with or without IL-1β (10 ng/ml). The expression and production of matrix metalloproteinase (MMP)-3, MMP-9, and MMP13 were determined using real-time PCR and Western blotting. CoQ10 suppressed MMP-3, MMP-9, and MMP13 production induced by IL-1β, and markedly inhibited IL-1β-induced MAPK pathways in rat chondrocytes. The present study provides insight into potential mechanisms by which CoQ10 protects against degeneration of cartilage in patients with OA, which may lead to new approaches for the treatment of OA.Drug Dev Res 78 : 403-410, 2017. © 2017 Wiley Periodicals, Inc.

Coenzyme Q10 Regulation of Apoptosis and Oxidative Stress in H2O2 Induced BMSC Death by Modulating the Nrf-2/NQO-1 Signaling Pathway and Its Application in a Model of Spinal Cord Injury

“…Cell Viability Assay. Cell viability was evaluated using a WST-1 assay [27]. In brief, BMSCs were seeded into a 96-well plate at a density of 1 × 10 4 /well.…”

“…Western Blotting Analysis. Western blotting experiments were performed following standard methods [27]. In brief, the proteins were loaded onto 10% SDS-PAGE and electrotransferred onto polyvinylidene difluoride membranes (Millipore, USA).…”

Berberine inhibits the interleukin‐1 beta‐induced inflammatory response via MAPK downregulation in rat articular chondrocytes

“…Previous studies showed that inhibition of the production of iNOS, COX‐2, and MMPs acted through the MAPK signaling pathway (Chen et al, ; Ma et al, ). Moreover, activating MAPKs has been associated with the progress of OA (Feng et al, ; Li et al, ). IL‐1β‐induced chondrocytes can increase the phosphorylation levels of p38, ERK, and JNK.…”

“…Cell viability was determined by WST‐1 assay according to the manufacturer's instructions (Li et al, ). Briefly, chondrocytes were cultured in a 96‐well plates (1 × 10 4 /well) for 24 hr.…”

“…The cDNA was synthesized using 1 μg of total RNA and reversed with a primescript‐RT reagent kit (TaKaRa Biotechnology Co. Ltd., Japan) according to the manufacturer's instructions; RT‐PCR was performed using the SYBR green system (Toyobo, Japan). The GAPDH gene was used as an internal control to evaluate relative gene expression (Li et al, ; Mao et al, ). Primer sequences are shown in Table .…”