Coexistence of Different Genotypes in the Same Bat and Serological Characterization of
            <i>Rousettus</i>
            Bat Coronavirus HKU9 Belonging to a Novel
            <i>Betacoronavirus</i>
            Subgroup

Lau, Susanna K. P.; Poon, Rosana W.S.; Wong, Beatrice H.L.; Wang, Ming; Huang, Yi; Xu, Huifang; Guo, Rong; Li, Kenneth S. M.; Gao, Kai; Chan, Kwok Hung; Zheng, Bo; Woo, Patrick C. Y.; Yuen, Kwok‐Yung

doi:10.1128/jvi.01121-10

Cited by 105 publications

(142 citation statements)

References 58 publications

Supporting

Mentioning

131

Contrasting

Unclassified

Order By: Relevance

“…Consistent with the Solexa data, a variety of HKU9 viruses were detected, but only in Hipposideros bat samples collected from Yunnan Province. Our results are consistent with a previous investigation in Hong Kong, China, conducted by Lau et al (22).…”

Section: Discussionsupporting

confidence: 83%

See 1 more Smart Citation

Metagenomic Analysis of Viruses from Bat Fecal Samples Reveals Many Novel Viruses in Insectivorous Bats in China

Yang

et al. 2012

J Virol

202

183

View full text Add to dashboard Cite

bIncreasing data indicate that bats harbor diverse viruses, some of which cause severe human diseases. In this study, sequenceindependent amplification and high-throughput sequencing (Solexa) were applied to the metagenomic analysis of viruses in bat fecal samples collected from 6 locations in China. A total of 8,746,417 reads with a length of 306,124,595 bp were obtained. Among these reads, 13,541 (0.15%) had similarity to phage sequences and 9,170 (0.1%) had similarity to eukaryotic virus sequences. A total of 129 assembled contigs (>100 nucleotides) were constructed and compared with GenBank: 32 contigs were related to phages, and 97 were related to eukaryotic viruses. The most frequent reads and contigs related to eukaryotic viruses were homologous to densoviruses, dicistroviruses, coronaviruses, parvoviruses, and tobamoviruses, a range that includes viruses from invertebrates, vertebrates, and plants. Most of the contigs had low identities to known viral genomic or protein sequences, suggesting that a large number of novel and genetically diverse insect viruses as well as putative mammalian viruses are transmitted by bats in China. This study provides the first preliminary understanding of the virome of some bat populations in China, which may guide the discovery and isolation of novel viruses in the future. Bats, the only flying mammal and the second most diverse group of mammals, are natural reservoirs of many emerging viruses (7, 38). Some bat viruses cause severe human diseases, such as lyssavirus, Hendra virus, Nipah virus, Ebola virus, and Marburg virus (16,23,29,41,47). Most of the known bat viruses have been discovered in apparently healthy bats (10,14,19,20,26,34,49). When bats are experimentally infected with henipavirus or the rabies virus, the bats shed the virus but do not produce any clinical syndrome like those observed in other animals and humans (1,17,30,40). This phenomenon may be due to the adaption of viruses to their host species, preinfection with related nonpathogenic viruses, or some unique characteristics of the bat immune system to viral infection. Considering the species richness and wide geographic distribution of bats, bats may be a source of many additional unknown viruses.The techniques for isolating viruses are straightforward but are not always successful because the majority of viruses are unable to grow in cell culture due to a lack of susceptible cell lines, a low titer of virus, or toxicity of environmental samples. PCR assays are sequence-dependent and extremely sensitive and rapid but have been complicated by the lack of a universally conserved gene or gene marker for all types of viruses. Recently, the sequence-independent amplification of viral nucleic acids followed by shotgun sequencing or pyrosequencing has been used to discover an enormous diversity of virus species and genotypes in marine and fresh water (2,6,12,37,39,45), human feces (3, 5, 43), animal tissues, and bat feces (11,13,24,32,42).In this study, six bat fecal samples were collected from six locations...

show abstract

Section: Discussionsupporting

confidence: 83%

“…7) (22). These results further demonstrate the genetic diversity of BtCoV HKU9 in bat populations (22).…”

Section: Solexa Sequencing Readsmentioning

confidence: 48%

Metagenomic Analysis of Viruses from Bat Fecal Samples Reveals Many Novel Viruses in Insectivorous Bats in China

Yang

et al. 2012

J Virol

202

183

View full text Add to dashboard Cite

show abstract

“…P. discolour is mainly nectarivorous and visits some of the same flowers as G. soricina (Kwiecinski, 2006), which may facilitate hypothetical exchange of viruses between the two bat genera. Sporadic observations of closely related CoVs in different bat species and even families were shown previously by us (Drexler et al, 2010) and other groups (Anthony et al, 2013;Lau et al, 2010). It remains unclear whether ecological factors such as population density and feeding habits influence the exchange of viruses between different bat species and the co-segregation of hosts and viruses.…”

Section: Dcrc5costamentioning

confidence: 85%

Highly diversified coronaviruses in neotropical bats

Corman

Rasche

Diallo

et al. 2013

Journal of General Virology

View full text Add to dashboard Cite

Bats host a broad diversity of coronaviruses (CoVs), including close relatives of human pathogens. There is only limited data on neotropical bat CoVs. We analysed faecal, blood and intestine specimens from 1562 bats sampled in Costa Rica, Panama, Ecuador and Brazil for CoVs by broad-range PCR. CoV RNA was detected in 50 bats representing nine different species, both frugivorous and insectivorous. These bat CoVs were unrelated to known human or animal pathogens, indicating an absence of recent zoonotic spill-over events. Based on RNAdependent RNA polymerase (RdRp)-based grouping units (RGUs) as a surrogate for CoV species identification, the 50 viruses represented five different alphacoronavirus RGUs and two betacoronavirus RGUs. Closely related alphacoronaviruses were detected in Carollia perspicillata and C. brevicauda across a geographical distance exceeding 5600 km. Our study expands the knowledge on CoV diversity in neotropical bats and emphasizes the association of distinct CoVs and bat host genera.

show abstract

“…As VP1 is the most surface-exposed virion protein and contains most of the motifs important for interaction with neutralizing antibodies and cellular receptors in picornaviruses (Racaniello, 2007), the sequence encoding the VP1 polypeptide of DcEV was cloned and the expressed recombinant protein was purified according to previously described strategies (Francis et al, 1987;Lau et al, 2005Lau et al, , 2010. To produce plasmid for expression of fusion protein containing affinity tags needed for its purification, primers 59-CTAGCTAGCGCACCAAAAGATGCTGTTCAG-39 and 59-GCCCT-TCTATAACAACAACCTGAGCTAGCTAG-39 were used to amplify the VP1 gene of DcEV by RT-PCR.…”

Section: Methodsmentioning

confidence: 99%

A novel dromedary camel enterovirus in the family Picornaviridae from dromedaries in the Middle East

Woo

Lau

et al. 2015

Journal of General Virology

Self Cite

View full text Add to dashboard Cite

The recent emergence of Middle East respiratory syndrome coronavirus from the Middle East and the discovery of the virus from dromedary camels have boosted interest in the search for novel viruses in dromedaries. Whilst picornaviruses are known to infect various animals, their existence in dromedaries was unknown. We describe the discovery of a novel picornavirus, dromedary camel enterovirus (DcEV), from dromedaries in Dubai. Among 215 dromedaries, DcEV was detected in faecal samples of four (1.9 %) dromedaries [one (0.5 %) adult dromedary and three (25 %) dromedary calves] by reverse transcription PCR. Analysis of two DcEV genomes showed that DcEV was clustered with other species of the genus Enterovirus and was most closely related to and possessed highest amino acid identities to the species Enterovirus E and Enterovirus F found in cattle. The G+C content of DcEV was 45 mol%, which differed from that of Enterovirus E and Enterovirus F (49-50 mol%) by 4-5 %. Similar to other members of the genus Enterovirus, the 59 UTR of DcEV possessed a putative type I internal ribosome entry site. The low ratios of the number of nonsynonymous substitutions per non-synonymous site to the number of synonymous substitutions per synonymous site (K a /K s ) of various coding regions suggested that dromedaries are the natural reservoir in which DcEV has been stably evolving. These results suggest that DcEV is a novel species of the genus Enterovirus in the family Picornaviridae. Western blot analysis using recombinant DcEV VP1 polypeptide showed a high seroprevalence of 52 % among serum samples from 172 dromedaries for IgG, concurring with its much higher infection rates in dromedary calves than in adults. Further studies are important to understand the pathogenicity, epidemiology and genetic evolution of DcEV in this unique group of animals.

show abstract

Coexistence of Different Genotypes in the Same Bat and Serological Characterization of Rousettus Bat Coronavirus HKU9 Belonging to a Novel Betacoronavirus Subgroup

Cited by 105 publications

References 58 publications

Metagenomic Analysis of Viruses from Bat Fecal Samples Reveals Many Novel Viruses in Insectivorous Bats in China

Metagenomic Analysis of Viruses from Bat Fecal Samples Reveals Many Novel Viruses in Insectivorous Bats in China

Highly diversified coronaviruses in neotropical bats

A novel dromedary camel enterovirus in the family Picornaviridae from dromedaries in the Middle East

Contact Info

Product

Resources

About