Coexistence of <i>Bos taurus</i> and <i>B. indicus</i> Mitochondrial DNAs in Nuclear Transfer-Derived Somatic Cattle Clones

Steinborn, Ralf; Schinogl, Pamela; Wells, David N.; Bergthaler, Andréas; Müller, Mathias; Brem, Gottfried

doi:10.1093/genetics/162.2.823

Cited by 71 publications

(1 citation statement)

References 35 publications

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“…Our results suggest that nuclei from mouse donor cells can completely support the biogenesis of mitochondria derived from porcine oocytes, and that mitochondria derived from mouse cells may not be seriously needed in late-stage reconstructed embryos and for implantation. These findings are consistent with the programming of mitochondria described for panda-rabbit cloned embryos [ 24 , 27 , 28 ].…”

Section: Discussionsupporting

confidence: 91%

Quantitative analysis of mitochondrial DNA in porcine-mouse cloned embryos

Shin¹,

Kim²,

Kim³

et al. 2023

J Anim Sci Technol

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The aim of the research is to identify that porcine oocytes can function as recipients for interspecies cloning and have the ability to develop to blastocysts. Furthermore each mitochondrial DNA (mtDNA) in interspecises cloned embryos was analyzed. For the study, mouse-porcine and porcine-porcine cloned embryos were produced with mouse fetal fibroblasts (MFF) and porcine fetal fibroblasts (PFF), respectively, introduced as donor cells into enucleated porcine oocytes. The developmental rate and cell numbers of blastocysts between intraspecies porcine-porcine and interspecies mouse-porcine cloned embryos were compared and real-time PCR was performed for the estimate of mouse and porcine mtDNA copy number in mouse-porcine cloned embryos at different stages.There was no significant difference in the developmental rate or total blastocyst number between mouse-porcine cloned embryos and porcine-porcine cloned embryos (11.1±0.9%, 25±3.5 vs. 10.1±1.2%, 24±6.3). In mouse-porcine reconstructed embryos, the copy numbers of mouse somatic cell-derived mtDNA decreased between the 1-cell and blastocyst stages, whereas the copy number of porcine oocyte-derived mtDNA significantly increased during this period, as assessed by realtime PCR analysis. In our real-time PCR analysis, we improved the standard curve construction-based method to analyze the level of mtDNA between mouse donor cells and porcine oocytes using the copy number of mouse beta-actin DNA as a standard. Our findings suggest that porcine-mouse cloned embryos have the ability to develop to blastocysts in vitro and exhibit mitochondrial heteroplasmy from the 1-cell to blastocyst stages and the mousederived mitochondria can be gradually replaced with those of the porcine oocyte in the early developmental stages of mouse-porcine cloned embryos.

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Section: Discussionsupporting

confidence: 91%

Quantitative analysis of mitochondrial DNA in porcine-mouse cloned embryos

Shin¹,

Kim²,

Kim³

et al. 2023

J Anim Sci Technol

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Genetic evidence of an Asian background in heteroplasmic Iberian cattle (Bos taurus): Effect on food authentication studies based on polymerase chain reaction-restriction fragment length polymorphism analysis

et al. 2005

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This work was aimed at identifying nucleotide polymorphic sites in a 359 bp region of the cytochrome b (cytb) mitochondrial gene of Iberian cattle (Bos taurus). This region is widely used as target in polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) species identification studies in foodstuffs destined for human and animal consumption. Two different coexisting restriction patterns were observed in four of the six animals studied when the 359 bp DNA fragment was cleaved with PalI, HinfI, MvaI, RsaI, or MboI. The amplification of both genotypes with the mitochondrial-specific primers L14735 and H15149 revealed the absence of nuclear pseudo-cytb genes, confirming the existence of mitochondrial heteroplasmy. The two coexisting mtDNA fragments were selectively sequenced in PCR extracts in which one genotype predominated over the other, both exhibiting a sequence variation of 10.4%. From the 37 nucleotide mismatches observed between genotypes, 32 were transitions and five were transversions. While 31 of the nucleotide mismatches between genotypes resulted to be conservative at the amino acid level, six changes implied amino acid substitutions, five of them being located in the variable transmembrane region. Genetic analysis suggests the presence of an Asian background in the mitochondria of Iberian cattle: while one of the genotypes matched the published sequence for Bos taurus, the other genotype clustered with a B. primigenius indicus animal and close to an Asian Bos taurus animal. These results also suggest that a number of current PCR-RFLP species identification methods based on cytb sequences may not be reliable for the accurate detection and identification of bovine material: an alternative battery of enzymes consisting of MmeI, NlaIV, and AluI is proposed.

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Tissue‐specific distribution of donor mitochondrial DNA in cloned mice produced by somatic cell nuclear transfer

Inoue

Ogonuki

Yamamoto

et al. 2004

Genesis

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Highly diverse results have been reported for mitochondrial DNA (mtDNA) hetero-plasmy in nuclear-transferred farm animals. In this study, we cloned genetically defined mice and investigated donor mtDNA inheritance following somatic cell cloning. Polymerase chain reaction (PCR) analysis with primers that were specific for either the recipient oocytes or donor cells revealed that the donor mtDNA coexisted with the recipient mtDNA in the brain, liver, kidney, and tail tissues of 96% (24/25) of the adult clones. When the proportion of donor mtDNA in each tissue was measured by allele-specific quantitative PCR and subjected to ANOVA analysis, a tissue-specific mtDNA segregation pattern (P < 0.05) was observed, with the liver containing the highest proportion of donor mtDNA. Therefore, the donor mtDNA was inherited consistently by the cloned offspring, whereas donor mtDNA segregation was not neutral, which is in accordance with previous notions about tissue-specific nuclear control of mtDNA segregation.

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Coexistence of Bos taurus and B. indicus Mitochondrial DNAs in Nuclear Transfer-Derived Somatic Cattle Clones

Cited by 71 publications

References 35 publications

Quantitative analysis of mitochondrial DNA in porcine-mouse cloned embryos

Quantitative analysis of mitochondrial DNA in porcine-mouse cloned embryos

Genetic evidence of an Asian background in heteroplasmic Iberian cattle (Bos taurus): Effect on food authentication studies based on polymerase chain reaction-restriction fragment length polymorphism analysis

Tissue‐specific distribution of donor mitochondrial DNA in cloned mice produced by somatic cell nuclear transfer

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