Coexistence of substance P- and methionine5-enkephalin-like immunoreactivity in nerve cells of the myenteric ganglia in the cat ileum

Domoto, Tokio; Gonda, Tatsuo; Oki, Makoto; Yanaihara, Noboru

doi:10.1016/0304-3940(84)90378-1

Cited by 28 publications

(26 citation statements)

References 17 publications

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“…It has been established that a significant proportion of myenteric plexus neurons contain an opioid peptide-like immunoreactivity (ALUMETS et al, 1978;LARSSON et al, 1979;BU' LocK et al, 1983;FURNESS et al, 1983b;DOMOTo et al, 1984;KOBAYASHI et al, 1984KOBAYASHI et al, , 1985NIHEI and IWANAGA,1985). These neurons are referred to as enkephalin neurons, because both methionineenkephalin and leucine-enkephalin, which were found by HUGHES et al (1975), are the best known opioid peptides.…”

Section: Discussionmentioning

confidence: 99%

Myenteric enkephalin neurons around the laser-photocoagulation necrosis: An immunocytochemical investigation in the guinea pig jejunum and proximal colon.

Kobayashi¹,

Nishisaka

1985

Arch. Histol. Jap., Arch. Histol. Jpn

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Section: Discussionmentioning

confidence: 99%

Myenteric enkephalin neurons around the laser-photocoagulation necrosis: An immunocytochemical investigation in the guinea pig jejunum and proximal colon.

Kobayashi¹,

Nishisaka

1985

Arch. Histol. Jap., Arch. Histol. Jpn

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“…Among them _Domoto er al. reported that about 10% of substance P (SP) immunopositive neurons in the cat ileum contained immunoreactive Metenkephalin (6). Costa er a1.…”

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confidence: 99%

“…They were present in the circular muscle layer, deep muscular plexus and submucous plexus. Difference in the projections of the three subclasses of the Met-Enk-Arg-Gly-Leu neurons may indicate that each of them performs different functions.Co-existence of two or more kinds of neuropeptides and amines in the enteric neurons have been demonstrated using sections (6,7, 12, 17) and whole mount preparations (4, 8) with light microscopic immunohistochemistry. Among them _Domoto er al.…”

mentioning

confidence: 99%

“…Co-existence of two or more kinds of neuropeptides and amines in the enteric neurons have been demonstrated using sections (6,7,12,17) and whole mount preparations (4,8) with light microscopic immunohistochemistry. Among them _Domoto er al.…”

mentioning

confidence: 99%

“…In the present study we examined the occurrence ofimmunoreactive SP and/or peptide-histidine-isoleucine (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15) (PHI (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)) in the Met-Enk-Arg-Gly-Leu neurons using the double staining protein A-colloidal gold method (1). We attempted to clarify the co-storage of Met-Enk-Arg-Gly-Leu with SP and/or PHI (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15) in the large cored vesicles with particular reference to those in the neuronal processes including terminal varicosities.…”

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confidence: 99%

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OCCURRENCE AND PROJECTIONS OF THREE SUBCLASSES OF MET-ENKEPHALIN-ARG6-GLY7-LEU8 NEURONS IN THE GUINEA-PIG DUODENUM: IMMUNOELECTRON MICROSCOPIC STUDY ON THE CO-STORAGE OF MET-ENKEPHALIN-ARG6- GLY7-LEU8 WITH SUBSTANCE P OR PHI (1-15)

Uchida¹,

Kobayashi²,

Yanaihara

1985

Biomed. Res.

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An immunoelectron microscopic study by the double staining protein A-colloidal gold method was performed on the neurons in the guinea-pig duodenum using specific antisera to neuropeptides, Le. Met-enkephalin-Arg°-Gly7-Leus (Met-Enk-Arg-Gly-Leu), substance P (SP) and peptide-histidine-isoleucine (1-15) (PHI (1-15)). Immunoreactive neuropeptides existed in the cored vesicles of 80-110 nm in diameter. The immunopositive cored vesicles were present in both somata and processes of some neurons. Occurrence and projections of three subclasses of Met-Enk-Arg-Gly-Leu immunopositive neurons (Met-Enk-Arg-Gly-Leu neurons) were described. Somata of the MetEnk-Arg-Gly-Leu neurons occurred in the myenteric ganglion. Processes of the Met-Enk-Arg-Gly-Leu neurons with immunoreactive SP projected to all the layers of the duodenal wall except the mucosa. Neuronal processes containing cored vesicles with both immunoreactive Met-Enk-Arg-Gly-Leu and PHI (1-15) were mainly distributed in the submucous plexus. None of them occurred around the duodenal gland. Neuronal processes containing cored vesicles with only immunoreactive MetEnk-Arg-Gly-Leu were small in number. They were present in the circular muscle layer, deep muscular plexus and submucous plexus. Difference in the projections of the three subclasses of the Met-Enk-Arg-Gly-Leu neurons may indicate that each of them performs different functions.Co-existence of two or more kinds of neuropeptides and amines in the enteric neurons have been demonstrated using sections (6,7, 12, 17) and whole mount preparations (4, 8) with light microscopic immunohistochemistry. Among them _Domoto er al. reported that about 10% of substance P (SP) immunopositive neurons in the cat ileum contained immunoreactive Metenkephalin (6). Costa er a1. investigated the coexistence of enkephalin, dynorphin and vasoactive intestinal polypeptide (VIP) in the neurons of the guinea-pig small intestine (4). However, because of the poor resolution of the light microscope, the objects of the previous studies were limited to the neuronal somata.We have recently shown the occurrence, projections, ultrastructure and synaptic connections of Met-enkephalin-Argf'-Gly7-Leu3 (Met-EnkArg-Gly-Leu) immunopositive neurons (MetEnk-Arg-Gly-Leu neurons) in the guinea-pig duodenum by the light and electron microscopic immunocytochemistry (11). In the present study we examined the occurrence ofimmunoreactive SP and/or peptide-histidine-isoleucine (1-15)

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Immunohistochemical identification of neurons in ganglia of the guinea pig sphincter of oddi

Wells¹,

Talmage²,

Mawe³

1995

J of Comparative Neurology

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The sphincter of Oddi is a smooth muscle sphincter that regulates the flow of bile into the duodenum. To identify potential chemical coding in sphincter of Oddi neurons, immunohistochemistry and histochemistry were employed to assay for putative neurotransmitters and related synthetic enzymes in wholemount preparations, with and without colchicine treatment. Immunoreactivities for enkephalin-endorphin (ENK-END), substance P (SP), nitric oxide synthase, vasoactive intestinal peptide (VIP), neuropeptide Y (NPY), and calcitonin gene-related peptide (CGRP) were demonstrated within the ganglionated plexus. Roughly half of the neurons in the sphincter of Oddi expressed immunoreactivity for both SP and ENK-END, but not for nitric oxide synthase. About 25% of the neurons expressed nitric oxide synthase immunoreactivity as well as NADPH-diaphorase activity. This contingent of neurons was made up of two subgroups: one that expressed immunoreactivity for VIP, the other for NPY. Neurons that expressed CGRP immunoreactivity were sparse in sphincter of Oddi ganglia; however, many axons immunoreactive for both CGRP and SP were present in the ganglionated plexus. The CGRP/SP fibers are probably visceral afferents that may influence ganglionic output through axon reflex circuits. These results, along with studies of the actions of these neuroactive compounds on sphincter tone, support the view that ganglia of the sphincter of Oddi are largely comprised of excitatory (SP/ENK-END-immunoreactive) and inhibitory (nitric oxide synthase/VIP- or NPY-immunoreactive) neurons, and that sphincter of Oddi tone is controlled by the regulation of the outputs of these two groups of cells.

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Coexistence of substance P- and methionine5-enkephalin-like immunoreactivity in nerve cells of the myenteric ganglia in the cat ileum

Cited by 28 publications

References 17 publications

Myenteric enkephalin neurons around the laser-photocoagulation necrosis: An immunocytochemical investigation in the guinea pig jejunum and proximal colon.

Myenteric enkephalin neurons around the laser-photocoagulation necrosis: An immunocytochemical investigation in the guinea pig jejunum and proximal colon.

Immunohistochemical identification of neurons in ganglia of the guinea pig sphincter of oddi

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