Coexpression of an intronic microRNA and its host gene reveals a potential role for miR-483-5p as an IGF2 partner

Ma, Ning; Wang, Xidi; Qiao, Yu; Li, Fuyuan; Yang, Hui; Zou, Chaoxia; Jin, Jianfeng; Lv, Guixiang; Peng, Yahui; Wang, Lujing; Huang, Hui; Zhou, Lingyun; Zheng, Xiaofei; Gao, Xu

doi:10.1016/j.mce.2010.11.027

Cited by 77 publications

(68 citation statements)

References 21 publications

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“…miR-483-5p is located in the second intron of IGF2, and the level of miR-483-5p correlates with IGF2 expression (Ma et al 2011). IGF2 is an imprinted gene that is expressed almost exclusively from the paternal allele in many tissues.…”

Section: Resultsmentioning

confidence: 99%

Human-specific regulation of MeCP2 levels in fetal brains by microRNA miR-483-5p

et al. 2013

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Proper neurological function in humans requires precise control of levels of the epigenetic regulator methyl CpG-binding protein 2 (MeCP2). MeCP2 protein levels are low in fetal brains, where the predominant MECP2 transcripts have an unusually long 3′ untranslated region (UTR). Here, we show that miR-483-5p, an intragenic microRNA of the imprinted IGF2, regulates MeCP2 levels through a human-specific binding site in the MECP2 long 3′ UTR. We demonstrate the inverse correlation of miR-483-5p and MeCP2 levels in developing human brains and fibroblasts from Beckwith-Wiedemann syndrome patients. Importantly, expression of miR-483-5p rescues abnormal dendritic spine phenotype of neurons overexpressing human MeCP2. In addition, miR-483-5p modulates the levels of proteins of the MeCP2-interacting corepressor complexes, including HDAC4 and TBL1X. These data provide insight into the role of miR-483-5p in regulating the levels of MeCP2 and interacting proteins during human fetal development.

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Section: Resultsmentioning

confidence: 99%

Human-specific regulation of MeCP2 levels in fetal brains by microRNA miR-483-5p

et al. 2013

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“…miR-483 has been reported to be expressed in mouse liver and adipocyte tissues and regulates fat metabolism by targeting SOCS3 (42). SOCS3 belongs to the SOCS family of inhibitory proteins, which can interfere with insulin signaling in muscle, liver, and adipose tissue by inhibition of tyrosine phosphorylation of IRS2 (56).…”

Section: Discussionmentioning

confidence: 99%

“…miR-483 is an intronic miRNA and is located within the intron of the Igf2 gene (42). IGF-2 was detected at a relatively high level in islets, and reduced IGF-2 expression was observed in the impaired islets of the adult Goto-Kakizaki rat model of type 2 diabetes (58).…”

Section: Discussionmentioning

confidence: 99%

Differentially Expressed MicroRNA-483 Confers Distinct Functions in Pancreatic β- and α-Cells

Mohan

Mao

Zhang

et al. 2015

Journal of Biological Chemistry

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Background: MicroRNAs are noncoding RNAs and have emerged as important regulators in ␤-cell function. Results: miR-483 is highly expressed in ␤-cells but expressed at much lower levels in ␣-cells, and increased miR-483 induces insulin production in ␤-cells while repressing glucagon production in ␣-cells. Conclusion: miR-483 has opposite effects in ␣-and ␤-cells by targeting SOCS3. Significance: miR-483 is a potential therapeutic target for diabetes.

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“…Its mRNA contains binding sites for 26 miRNAs, so its expression depends on several host genes that are coexpressed with their miRNA (Hoeppner et al, 2009;Ma et al, 2011;He et al, 2012;Li et al, 2015), or are dependent on the expression of miRNA genes that are located between protein-coding genes.…”

Section: Molecular and Cell Biology Vavilov Journal Of Genetics And Bmentioning

confidence: 99%

The Characteristics of Mirna Binding Sites in Mrna of Zfhx3 Gene and Its Orthologs

Kondybaeva¹,

Акимниязова²,

Kamenova³

et al. 2018

Vestn. VOGiS

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Transcription factor gene ZFHX3 is one of the candidate genes involved in stroke development. The ZFHX3 protein contains oligopeptides encoded by trinucleotide repeats (TNRs). TNR variability is considered to be one of the causes of the disease, but their biological function has not yet been established. We assume that TNRs are the binding sites of miRNA to mRNA and are involved in regulation of ZFHX3 gene expression. The characteristics of miRNA–mRNA interaction were determined using MirTarget software. It has been shown that the first TNR in mRNA of the human ZFHX3 gene consists of the seven consecutive miR-12-32603-3p binding encoding polyGlu. The ZFHX3 protein of human polyGlu contains 30 Glu. In the orthologous proteins of 36 animal species the length of polyGlu varied from 27 Glu to 33 Glu. Negatively charged polyGlu of the ZFHX3 transcription factor probably interacted with positive DNA-binding proteins. The following mRNA region of the ZFHX3 gene contained the binding sites for miR-17-39416-3p, miR-5-15733-3p, miR-9-20317-3 encoding polyAla by 15 Ala lengths. In the 33 ZFHX3 orthologous proteins polyAla had the same length. The mRNA region of the human ZFHX3 gene with binding polysite of miR-1322-3p encoded polyGln consisting of 19 Gln. In the 41 orthologs of the ZFHX3 protein the length of polyGln varied from seven Gln to 23 Gln. The binding sites of miR-2-6184-3p, miR-5-14114-5p and miR-19-43437-5p were located with overlapping nucleotides sequences, and encode polyPro. In ZFHX3 human polyPro consisted of 12 Pro. In the orthologs, polyPro contained from 10 Pro to 14 Pro. The binding sites of miR-17-39416-3p, miR-9-20317-3p, miR-1-1819-3p, miR-5-15733-3p, miR-6-17815-3p, miR-18-39953-5p, miR-26862-5p, miR-1260b and miR-X-48174-3p in human ZFHX3 encoded polyGly by 22 Gly length. In the 28 orthologs of ZFHX3 the length of polyGly decreased to 11 Gly. The TNR regions could simultaneously bind several miRNAs, which increased the dependence of gene expression on miRNA. The oligopeptides encoded by the binding polysites of miRNA in mRNA in the orthologous ZFHX3 proteins were flanked by conserved oligopeptides.

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Coexpression of an intronic microRNA and its host gene reveals a potential role for miR-483-5p as an IGF2 partner

Cited by 77 publications

References 21 publications

Human-specific regulation of MeCP2 levels in fetal brains by microRNA miR-483-5p

Human-specific regulation of MeCP2 levels in fetal brains by microRNA miR-483-5p

Differentially Expressed MicroRNA-483 Confers Distinct Functions in Pancreatic β- and α-Cells

The Characteristics of Mirna Binding Sites in Mrna of Zfhx3 Gene and Its Orthologs

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