Coexpression of CAV-1, AT1-R and FOXM1 in prostate and breast cancer and normal cell lines and their influence on metastatic properties

Abstract

The aim of this study was to evaluate the coexpression of caveolin-1 (CAV-1), angiotensin II type 1 receptor (AT1-R) and forkhead box Ml (FOXM1) in prostate and breast cancer cell lines, in comparison with normal cell lines. CAV-1, AT1-R and FOXM1 expression was determined by reverse transcription-quantitative polymerase chain reaction and western blot analysis in the prostate cancer cell lines PC3, DU145 and LNCaP; prostate normal cell line PNT1A; breast cancer cell lines MCF-7 and MDA-MB-231; and the normal breast cell line 184A1. A correlation between the expression levels of the investigated genes and their metastatic properties was determined by the Spearman's rank test (P<0.05) and Aspin-Welsch t-test, respectively. In prostate cell lines, a significant correlation was noted between CAV-1 and AT1-R expression and between FOXM1 and CAV-1 expression. A correlation between the expression levels of the investigated genes and their metastatic potential was also observed, with relatively high expression of all the investigated genes in the normal prostate cell line PNT1A. In comparison to prostate cancer cell lines, an adverse dependency between CAV-1, AT1-R, FOXM1 expression and metastatic potential was observed in the breast cancer cell lines. Relatively high expression of all tested genes was observed in the normal breast cell line 184A1, which was decreasing respectively with increasing metastatic potential of breast cancer cell lines. The results obtained here indicate that CAV-1, FOXM1 and AT1-R may be potential markers of tumorigenesis in certain types of cancer in vitro.

Elevation of androgen receptor promotes prostate cancer metastasis by induction of epithelial‐mesenchymal transition and reduction of KAT5

“…As several AR coregulators are also involved in regulation of PCa progression of cancer metastasis, we examined whether alteration of AR have previously been reported to be involved in the regulation of PCa metastasis, 32,33 we compared the abundance of these proteins in control and AR knockdown C4-2B cells. Knockdown of AR decreased protein level of CAV1 ( Figure S1) but increased abundance of KAT5 protein ( Figure 6B).…”

A Practical Guide to High‐resolution X‐ray Spectroscopic Measurements and their Applications in Bioinorganic Chemistry

“…An XAS spectrum results from the measurement of thea bsorption coefficient of an elementa s af unction of incidente nergy.T his is realized either directly,b ym easuringt he number of photonst ransmitted through the sample,o ri ndirectly,b ym easuring the number of fluorescent photons after ac ore level electron is excited by incidenth igh energy X-ray photons.I ft he electron originates from the 1s level it is referred to as Kedge XAS; similarly,e xcitationf rom 2s and 2p levels correspond to L-edges,a nd core excitation from 3s,3 p, and 3d levels are referred to as M-edges ( Figure 1). [13,14,17] An XAS spectrum is generally divided into two regions:t he X-raya bsorption near edge structure (XANES), or edge region,a nd the extended X-raya bsorptionf ine structure region ( Figure 2, left).…”

“…Ty pically,ashift of 1-2 eV corresponds to ac hange in 1u nit oxidation state. [13,14] Thep re-edge intensity additionally provides information about the symmetry of the system. In non-centrosymmetric point groups,3 da nd 4p orbitals can mix, providing dipole-allowedc ontributions, hence the pre-edge gains intensity,a sc ompared with centrosymmetric systems.…”

“…In this regard, X-ray spectroscopy has played ap articularly pivotalrole,inlarge part due to its element selectivity and the ability to obtain both oxidation state and metrical parameters,f rom the X-raya bsorption( XAS)e dge and extended X-ray absorption fine structure (EXAFS) regions,r espectively. [13,14] Since the advent of synchrotron facilities, XAS has provided key insights into the structure of metalloprotein active sites. Examples of particular note include the earliest proposalsf or the active site structures of the Mn 4 CaO 5 oxygen-evolving complex of photosystem II [8] and of the iron-molybdenum cofactor of nitrogenase.…”

Lower levels of Caveolin-1 and higher levels of endothelial nitric oxide synthase are observed in abdominal aortic aneurysm patients treated with simvastatin

“…Caveolin-1 (Cav-1) is a structural protein of the caveolae known to possess a functional role in cell signalling (Kowalska et al, 2016). In endothelial cells, Cav-1 participates in the regulation of eNOS signalling via modulation of eNOS activation.…”